A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus
Hepatitis A virus (HAV), a major cause of acute liver infections, is transmitted through the fecal–oral route and close contact with infected individuals. Current HAV standardized methods rely on the detection of virus antigen or RNA, which do not differentiate between infectious and non-infectious...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/14/12/576 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850049994441097216 |
|---|---|
| author | Dilmeet Kaur Malak A. Esseili Ramaraja P. Ramasamy |
| author_facet | Dilmeet Kaur Malak A. Esseili Ramaraja P. Ramasamy |
| author_sort | Dilmeet Kaur |
| collection | DOAJ |
| description | Hepatitis A virus (HAV), a major cause of acute liver infections, is transmitted through the fecal–oral route and close contact with infected individuals. Current HAV standardized methods rely on the detection of virus antigen or RNA, which do not differentiate between infectious and non-infectious HAV. The objective of this study was to develop a prototype cell-based electrochemical biosensor for detection of infectious HAV. A cell culture-adapted HAV strain (HM175/18f) and its permissive cells (FRhK-4), along with gold nanoparticle-modified screen-printed electrodes, were used to develop the biosensor. Electrochemical impedance spectroscopy was used to quantify the electrical impedance signal. Nyquist plots showed successful fabrication of the cell-based biosensor. The optimum period of HAV incubation with the biosensor was 6 h. A significant linear relationship (R<sup>2</sup> = 0.98) was found between the signal and a 6-log range of HAV titers, with a limit of detection of ~5 TCID<sub>50</sub>/mL (tissue culture infectious dose). The biosensor did not detect non-target viruses such as feline calicivirus and human coronavirus 229E. The biosensor was stable for 3 to 7 days at an abusive temperature (37 °C), retaining ~90 to 60% of the original signal, respectively. In conclusion, this prototype cell-based biosensor is capable of rapidly detecting low levels of infectious HAV. |
| format | Article |
| id | doaj-art-d2a6bb9e5ece4298bee2ae330e85d727 |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-d2a6bb9e5ece4298bee2ae330e85d7272025-08-20T02:53:34ZengMDPI AGBiosensors2079-63742024-11-01141257610.3390/bios14120576A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A VirusDilmeet Kaur0Malak A. Esseili1Ramaraja P. Ramasamy2Nano Electrochemistry Laboratory, College of Engineering, University of Georgia, Athens, GA 30602, USACenter for Food Safety, University of Georgia, Griffin Campus, Griffin, GA 30223, USANano Electrochemistry Laboratory, College of Engineering, University of Georgia, Athens, GA 30602, USAHepatitis A virus (HAV), a major cause of acute liver infections, is transmitted through the fecal–oral route and close contact with infected individuals. Current HAV standardized methods rely on the detection of virus antigen or RNA, which do not differentiate between infectious and non-infectious HAV. The objective of this study was to develop a prototype cell-based electrochemical biosensor for detection of infectious HAV. A cell culture-adapted HAV strain (HM175/18f) and its permissive cells (FRhK-4), along with gold nanoparticle-modified screen-printed electrodes, were used to develop the biosensor. Electrochemical impedance spectroscopy was used to quantify the electrical impedance signal. Nyquist plots showed successful fabrication of the cell-based biosensor. The optimum period of HAV incubation with the biosensor was 6 h. A significant linear relationship (R<sup>2</sup> = 0.98) was found between the signal and a 6-log range of HAV titers, with a limit of detection of ~5 TCID<sub>50</sub>/mL (tissue culture infectious dose). The biosensor did not detect non-target viruses such as feline calicivirus and human coronavirus 229E. The biosensor was stable for 3 to 7 days at an abusive temperature (37 °C), retaining ~90 to 60% of the original signal, respectively. In conclusion, this prototype cell-based biosensor is capable of rapidly detecting low levels of infectious HAV.https://www.mdpi.com/2079-6374/14/12/576cell-based biosensorsinfectious virusesHepatitis A virusinfectivity assayFRhK-4 cellsfoodborne outbreaks |
| spellingShingle | Dilmeet Kaur Malak A. Esseili Ramaraja P. Ramasamy A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus Biosensors cell-based biosensors infectious viruses Hepatitis A virus infectivity assay FRhK-4 cells foodborne outbreaks |
| title | A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus |
| title_full | A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus |
| title_fullStr | A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus |
| title_full_unstemmed | A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus |
| title_short | A Cell-Based Electrochemical Biosensor for the Detection of Infectious Hepatitis A Virus |
| title_sort | cell based electrochemical biosensor for the detection of infectious hepatitis a virus |
| topic | cell-based biosensors infectious viruses Hepatitis A virus infectivity assay FRhK-4 cells foodborne outbreaks |
| url | https://www.mdpi.com/2079-6374/14/12/576 |
| work_keys_str_mv | AT dilmeetkaur acellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus AT malakaesseili acellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus AT ramarajapramasamy acellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus AT dilmeetkaur cellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus AT malakaesseili cellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus AT ramarajapramasamy cellbasedelectrochemicalbiosensorforthedetectionofinfectioushepatitisavirus |