EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS
Goal of the study: to investigate the impact of isoniazid combined with silver nanoparticles on the isoniazid resistant strains of tuberculous mycobacteria and the course of experimental of tuberculosis caused by the above strains.Materials and methods. The electrochemical metal resolution was...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
New Terra Publishing House
2017-06-01
|
| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/1014 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850223483542306816 |
|---|---|
| author | A. V. Zаkhаrov A. E. Ergeshov A. L. Khokhlov B. S. Kibrik |
| author_facet | A. V. Zаkhаrov A. E. Ergeshov A. L. Khokhlov B. S. Kibrik |
| author_sort | A. V. Zаkhаrov |
| collection | DOAJ |
| description | Goal of the study: to investigate the impact of isoniazid combined with silver nanoparticles on the isoniazid resistant strains of tuberculous mycobacteria and the course of experimental of tuberculosis caused by the above strains.Materials and methods. The electrochemical metal resolution was used to obtain silver nanoparticles, the average size of nanoparticles made 3-60 nm. The investigated concentrations of silver nanoparticles made 5; 25; 50 µg/ml. The isoniazid was used only in one concentration of 1 mg/ml. Totally there were 651 in vitro tests.The experimental tuberculosis model included infecting mice (totally 68) with two-week virulent multiple drug resistant culture of M. tuberculosis.Results. In vitro tests proved that isoniazid combined with silver nanoparticles fully or significantly suppressed the growth of MDR TB strain in 49.2% of cases. The minimal inhibitory concentration of nanoparticles in the combination with isoniazid made 2.5 µg/ml and minimal bactericidal concentration made 5 µg/ml. Atomic force microscopy detected the changes in morphometric parameters of MDR tuberculous mycobacteria after exposure to silver nanoparticles combined with isoniazid unlike the use of isoniazid only or silver nanoparticles. When treating experimental tuberculosis, survival rates and histological tests of the lung tissue confirmed that the combination of isoniazid and silver nanoparticles was preferable compared to the single use of the above components. |
| format | Article |
| id | doaj-art-d2a5ce84981b42a4910e47390eaa2cb5 |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2017-06-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-d2a5ce84981b42a4910e47390eaa2cb52025-08-20T02:05:56ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062017-06-01956515810.21292/2075-1230-2017-95-6-51-581014EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSISA. V. Zаkhаrov0A. E. Ergeshov1A. L. Khokhlov2B. S. Kibrik3Regional Clinical Tuberculosis HospitalCentral Tuberculosis Research InstituteYaroslavl State Medical UniversityYaroslavl State Medical UniversityGoal of the study: to investigate the impact of isoniazid combined with silver nanoparticles on the isoniazid resistant strains of tuberculous mycobacteria and the course of experimental of tuberculosis caused by the above strains.Materials and methods. The electrochemical metal resolution was used to obtain silver nanoparticles, the average size of nanoparticles made 3-60 nm. The investigated concentrations of silver nanoparticles made 5; 25; 50 µg/ml. The isoniazid was used only in one concentration of 1 mg/ml. Totally there were 651 in vitro tests.The experimental tuberculosis model included infecting mice (totally 68) with two-week virulent multiple drug resistant culture of M. tuberculosis.Results. In vitro tests proved that isoniazid combined with silver nanoparticles fully or significantly suppressed the growth of MDR TB strain in 49.2% of cases. The minimal inhibitory concentration of nanoparticles in the combination with isoniazid made 2.5 µg/ml and minimal bactericidal concentration made 5 µg/ml. Atomic force microscopy detected the changes in morphometric parameters of MDR tuberculous mycobacteria after exposure to silver nanoparticles combined with isoniazid unlike the use of isoniazid only or silver nanoparticles. When treating experimental tuberculosis, survival rates and histological tests of the lung tissue confirmed that the combination of isoniazid and silver nanoparticles was preferable compared to the single use of the above components.https://www.tibl-journal.com/jour/article/view/1014экспериментальный туберкулезнаночастицы серебра |
| spellingShingle | A. V. Zаkhаrov A. E. Ergeshov A. L. Khokhlov B. S. Kibrik EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS Туберкулез и болезни лёгких экспериментальный туберкулез наночастицы серебра |
| title | EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS |
| title_full | EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS |
| title_fullStr | EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS |
| title_full_unstemmed | EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS |
| title_short | EFFECTIVENESS OF COMBINATION OF ISONIAZID AND SILVER NANOPARTICLES IN THE TREATMENT OF EXPERIMENTAL TUBERCULOSIS |
| title_sort | effectiveness of combination of isoniazid and silver nanoparticles in the treatment of experimental tuberculosis |
| topic | экспериментальный туберкулез наночастицы серебра |
| url | https://www.tibl-journal.com/jour/article/view/1014 |
| work_keys_str_mv | AT avzakharov effectivenessofcombinationofisoniazidandsilvernanoparticlesinthetreatmentofexperimentaltuberculosis AT aeergeshov effectivenessofcombinationofisoniazidandsilvernanoparticlesinthetreatmentofexperimentaltuberculosis AT alkhokhlov effectivenessofcombinationofisoniazidandsilvernanoparticlesinthetreatmentofexperimentaltuberculosis AT bskibrik effectivenessofcombinationofisoniazidandsilvernanoparticlesinthetreatmentofexperimentaltuberculosis |