Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil
Cetacean morbillivirus (CeMV) is a major threat to cetaceans worldwide, causing individual deaths and outbreaks of mass mortality. Based on partial sequences of the viral phosphoprotein, CeMV is subclassified into seven strains and two distinct lineages. To date, only CeMV-1 strains, including the d...
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| author | Kátia Regina Groch Sueli Akemi Taniwaki Miyagi Josué Díaz-Delgado Elitieri B. Santos-Neto José Lailson-Brito Paulo Eduardo Brandão José Luiz Catão-Dias |
| author_facet | Kátia Regina Groch Sueli Akemi Taniwaki Miyagi Josué Díaz-Delgado Elitieri B. Santos-Neto José Lailson-Brito Paulo Eduardo Brandão José Luiz Catão-Dias |
| author_sort | Kátia Regina Groch |
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| description | Cetacean morbillivirus (CeMV) is a major threat to cetaceans worldwide, causing individual deaths and outbreaks of mass mortality. Based on partial sequences of the viral phosphoprotein, CeMV is subclassified into seven strains and two distinct lineages. To date, only CeMV-1 strains, including the dolphin morbillivirus (DMV), have been completely sequenced. The CeMV-2 lineage was first reported in Guiana dolphins (<i>Sotalia guianensis</i>) in Brazil and was associated with an unusual mortality event in 2017–2018. Here we provide the nearly complete Guiana dolphin morbillivirus (GDMV) genome sequence, representing the first within the CeMV-2 lineage. GDMV was isolated using Vero.DogSLAMtag cells, the viral RNA was extracted, and deep sequencing analysis was performed. Gaps in the viral genome were completed by Sanger sequencing. The final genome length was 15,607 nucleotides covering 99.3% of the DMV reference genome, including full sequences of the six structural proteins encoded by morbillivirus. The sequence similarity was 74–77.9% to other CeMV strains, with highest identity to the DMV. The complete L protein amino acid sequence comparison-based taxonomy indicates that GDMV is a distinct morbillivirus species; however, as GDMV and CeMV-1 strains infect a similar host spectrum, our findings support that GDMV represents a new CeMV-2 lineage. |
| format | Article |
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| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Viruses |
| spelling | doaj-art-d2a4d68eb0bc4ed38becbd877b33f0332025-08-20T03:13:48ZengMDPI AGViruses1999-49152025-04-0117458210.3390/v17040582Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), BrazilKátia Regina Groch0Sueli Akemi Taniwaki Miyagi1Josué Díaz-Delgado2Elitieri B. Santos-Neto3José Lailson-Brito4Paulo Eduardo Brandão5José Luiz Catão-Dias6Department of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, BrazilDepartment of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, BrazilLaboratório de Mamíferos Aquáticos e Bioindicadores (MAQUA), Faculdade de Oceanografia, Rio de Janeiro State University (UERJ), Rio de Janeiro 20550-013, BrazilLaboratório de Mamíferos Aquáticos e Bioindicadores (MAQUA), Faculdade de Oceanografia, Rio de Janeiro State University (UERJ), Rio de Janeiro 20550-013, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, BrazilDepartment of Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-207, BrazilCetacean morbillivirus (CeMV) is a major threat to cetaceans worldwide, causing individual deaths and outbreaks of mass mortality. Based on partial sequences of the viral phosphoprotein, CeMV is subclassified into seven strains and two distinct lineages. To date, only CeMV-1 strains, including the dolphin morbillivirus (DMV), have been completely sequenced. The CeMV-2 lineage was first reported in Guiana dolphins (<i>Sotalia guianensis</i>) in Brazil and was associated with an unusual mortality event in 2017–2018. Here we provide the nearly complete Guiana dolphin morbillivirus (GDMV) genome sequence, representing the first within the CeMV-2 lineage. GDMV was isolated using Vero.DogSLAMtag cells, the viral RNA was extracted, and deep sequencing analysis was performed. Gaps in the viral genome were completed by Sanger sequencing. The final genome length was 15,607 nucleotides covering 99.3% of the DMV reference genome, including full sequences of the six structural proteins encoded by morbillivirus. The sequence similarity was 74–77.9% to other CeMV strains, with highest identity to the DMV. The complete L protein amino acid sequence comparison-based taxonomy indicates that GDMV is a distinct morbillivirus species; however, as GDMV and CeMV-1 strains infect a similar host spectrum, our findings support that GDMV represents a new CeMV-2 lineage.https://www.mdpi.com/1999-4915/17/4/582cetacean morbilliviruscell culturevirus isolationmarine mammal strandingmortality outbreakepizootics |
| spellingShingle | Kátia Regina Groch Sueli Akemi Taniwaki Miyagi Josué Díaz-Delgado Elitieri B. Santos-Neto José Lailson-Brito Paulo Eduardo Brandão José Luiz Catão-Dias Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil Viruses cetacean morbillivirus cell culture virus isolation marine mammal stranding mortality outbreak epizootics |
| title | Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil |
| title_full | Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil |
| title_fullStr | Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil |
| title_full_unstemmed | Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil |
| title_short | Complete Genome Sequencing of the Divergent Guiana Dolphin Morbillivirus (GDMV), Brazil |
| title_sort | complete genome sequencing of the divergent guiana dolphin morbillivirus gdmv brazil |
| topic | cetacean morbillivirus cell culture virus isolation marine mammal stranding mortality outbreak epizootics |
| url | https://www.mdpi.com/1999-4915/17/4/582 |
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