Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea
Metatranscriptome analysis and reverse transcription polymerase chain reaction diagnostics were conducted to investigate viral disease in 75 Narcissus samples, resulting in the detection of snowdrop virus Y (SVY). The complete genome sequence of the SVY isolate SA66 was 10,560 nt in length, and sequ...
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Hanrimwon Publishing Company
2024-12-01
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| Series: | Research in Plant Disease |
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| Online Access: | http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-409.pdf |
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| author | Minseok Kim Minhui Kim Sangmin Bak Hak ju Kim Hong-Kyu Lee Jean Geung Min Taegun Lim Seungbin Baek Minjoo Yang Dong-Joo Min Jin-Sung Hong Hee-Seong Byun Kyunghwa Nam Su-Heon Lee |
| author_facet | Minseok Kim Minhui Kim Sangmin Bak Hak ju Kim Hong-Kyu Lee Jean Geung Min Taegun Lim Seungbin Baek Minjoo Yang Dong-Joo Min Jin-Sung Hong Hee-Seong Byun Kyunghwa Nam Su-Heon Lee |
| author_sort | Minseok Kim |
| collection | DOAJ |
| description | Metatranscriptome analysis and reverse transcription polymerase chain reaction diagnostics were conducted to investigate viral disease in 75 Narcissus samples, resulting in the detection of snowdrop virus Y (SVY). The complete genome sequence of the SVY isolate SA66 was 10,560 nt in length, and sequence analysis revealed a typical genome structure for Potyvirus. Electron microscopy revealed filamentous rod particles approximately 800 nm long in the infected samples. In the bioassay involving 19 test plants, no SVY infection was confirmed. The coat protein sequences of 10 SVY isolates from various samples were determined, showing nucleotide identities of 99.3-99.8% and amino acid identities of 98.9-99.6% when compared with three previously reported isolates. The complete genome sequence shared 99.6% nucleotide identity and 99.7% amino acid identity with the reported isolate. SVY was detected in 10 of the collected samples, specifically showing partial detection in all four cultivars from the Sinan region, while it was not detected in the other three regions. This study is the first report of SVY in Korea and is expected to contribute to the quarantine of Narcissus bulbs and to the production of virus-free bulbs. |
| format | Article |
| id | doaj-art-d2a2b851bf484e2e95228057a05801f3 |
| institution | DOAJ |
| issn | 1598-2262 2233-9191 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj-art-d2a2b851bf484e2e95228057a05801f32025-08-20T03:06:14ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912024-12-0130440942010.5423/RPD.2024.30.4.4091874Identification of Snowdrop Virus Y Occurred on Narcissus spp. in KoreaMinseok Kim0Minhui Kim1Sangmin Bak2Hak ju Kim3Hong-Kyu Lee4Jean Geung Min5Taegun Lim6Seungbin Baek7Minjoo Yang8Dong-Joo Min9Jin-Sung Hong10Hee-Seong Byun11Kyunghwa Nam12Su-Heon Lee13School of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaInterdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, KoreaInterdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, KoreaCrop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu 41566, KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu 41566, KoreaMetatranscriptome analysis and reverse transcription polymerase chain reaction diagnostics were conducted to investigate viral disease in 75 Narcissus samples, resulting in the detection of snowdrop virus Y (SVY). The complete genome sequence of the SVY isolate SA66 was 10,560 nt in length, and sequence analysis revealed a typical genome structure for Potyvirus. Electron microscopy revealed filamentous rod particles approximately 800 nm long in the infected samples. In the bioassay involving 19 test plants, no SVY infection was confirmed. The coat protein sequences of 10 SVY isolates from various samples were determined, showing nucleotide identities of 99.3-99.8% and amino acid identities of 98.9-99.6% when compared with three previously reported isolates. The complete genome sequence shared 99.6% nucleotide identity and 99.7% amino acid identity with the reported isolate. SVY was detected in 10 of the collected samples, specifically showing partial detection in all four cultivars from the Sinan region, while it was not detected in the other three regions. This study is the first report of SVY in Korea and is expected to contribute to the quarantine of Narcissus bulbs and to the production of virus-free bulbs.http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-409.pdffirst reportnarcissussequence analysissnowdrop virus y |
| spellingShingle | Minseok Kim Minhui Kim Sangmin Bak Hak ju Kim Hong-Kyu Lee Jean Geung Min Taegun Lim Seungbin Baek Minjoo Yang Dong-Joo Min Jin-Sung Hong Hee-Seong Byun Kyunghwa Nam Su-Heon Lee Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea Research in Plant Disease first report narcissus sequence analysis snowdrop virus y |
| title | Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea |
| title_full | Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea |
| title_fullStr | Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea |
| title_full_unstemmed | Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea |
| title_short | Identification of Snowdrop Virus Y Occurred on Narcissus spp. in Korea |
| title_sort | identification of snowdrop virus y occurred on narcissus spp in korea |
| topic | first report narcissus sequence analysis snowdrop virus y |
| url | http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-409.pdf |
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