Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs
Abstract Adoption of microbial preparations is becoming more and more prevalent in the pig breeding industry. Digestive tract microbes are recognized as crucial elements affecting physical characteristics of pigs. Until now, it is still challenging to establish connections between colonic microbiome...
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Animal Research and One Health |
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| Online Access: | https://doi.org/10.1002/aro2.39 |
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| author | Xiaoyu Huang Huihui Li Feng Cheng Ligang Wang Guoqing Cao Lixian Wang Lijun Shi |
| author_facet | Xiaoyu Huang Huihui Li Feng Cheng Ligang Wang Guoqing Cao Lixian Wang Lijun Shi |
| author_sort | Xiaoyu Huang |
| collection | DOAJ |
| description | Abstract Adoption of microbial preparations is becoming more and more prevalent in the pig breeding industry. Digestive tract microbes are recognized as crucial elements affecting physical characteristics of pigs. Until now, it is still challenging to establish connections between colonic microbiome and the host. In this study, weight gain models were created for Min and Yorkshire pigs. The colonic contents and colonic tissues were collected from two pig purebred strains (n = 8/group) with similar weights for multi‐omics analysis. By difference analysis of colonic microbiota, Min pigs observed a significantly higher relative abundance of Bacteroides, Phocaeicola, Roseburia, and Parabacteroides, and Yorkshire pigs had a strongly higher relative abundance of Streptococcus, Vescimonas, Ligilactobacillus, and Lactococcus. Functional annotation showed that the colonic microbiota of Min pigs had extensive dietary polysaccharide and immunomodulatory capacity. Through the correlation analysis of colonic microbiota with metabolomics of colonic content or transcriptomics of colonic tissues, we provided direct and indirect relationships of microorganisms and hosts. By verification, the contents of Occludin, ZO‐1, and pIgR in colonic tissue and sIgA, sIgG, and sIgM in colonic contents of Min pigs were significantly higher than that in Yorkshire pigs. This study revealed characteristics and functions of the colonic microbiota in Min and Yorkshire pigs and analyzed their interactions with the host. Also, we identified immune‐related microorganisms. These results provided a theoretical basis for understanding the influence of colonic microbiota on phenotype shaping in pigs. |
| format | Article |
| id | doaj-art-d2a0a19832004ed2be5eda384671da49 |
| institution | DOAJ |
| issn | 2835-5075 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Research and One Health |
| spelling | doaj-art-d2a0a19832004ed2be5eda384671da492025-08-20T03:02:52ZengWileyAnimal Research and One Health2835-50752025-08-013327829610.1002/aro2.39Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigsXiaoyu Huang0Huihui Li1Feng Cheng2Ligang Wang3Guoqing Cao4Lixian Wang5Lijun Shi6State Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaCollege of Animal Science Shanxi Agricultural University Jinzhong ChinaState Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Animal Biotech Breeding Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing ChinaAbstract Adoption of microbial preparations is becoming more and more prevalent in the pig breeding industry. Digestive tract microbes are recognized as crucial elements affecting physical characteristics of pigs. Until now, it is still challenging to establish connections between colonic microbiome and the host. In this study, weight gain models were created for Min and Yorkshire pigs. The colonic contents and colonic tissues were collected from two pig purebred strains (n = 8/group) with similar weights for multi‐omics analysis. By difference analysis of colonic microbiota, Min pigs observed a significantly higher relative abundance of Bacteroides, Phocaeicola, Roseburia, and Parabacteroides, and Yorkshire pigs had a strongly higher relative abundance of Streptococcus, Vescimonas, Ligilactobacillus, and Lactococcus. Functional annotation showed that the colonic microbiota of Min pigs had extensive dietary polysaccharide and immunomodulatory capacity. Through the correlation analysis of colonic microbiota with metabolomics of colonic content or transcriptomics of colonic tissues, we provided direct and indirect relationships of microorganisms and hosts. By verification, the contents of Occludin, ZO‐1, and pIgR in colonic tissue and sIgA, sIgG, and sIgM in colonic contents of Min pigs were significantly higher than that in Yorkshire pigs. This study revealed characteristics and functions of the colonic microbiota in Min and Yorkshire pigs and analyzed their interactions with the host. Also, we identified immune‐related microorganisms. These results provided a theoretical basis for understanding the influence of colonic microbiota on phenotype shaping in pigs.https://doi.org/10.1002/aro2.39biomarkerscolonic microorganismMin pigsMulti‐omicsYorkshire pigs |
| spellingShingle | Xiaoyu Huang Huihui Li Feng Cheng Ligang Wang Guoqing Cao Lixian Wang Lijun Shi Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs Animal Research and One Health biomarkers colonic microorganism Min pigs Multi‐omics Yorkshire pigs |
| title | Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs |
| title_full | Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs |
| title_fullStr | Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs |
| title_full_unstemmed | Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs |
| title_short | Multi‐omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs |
| title_sort | multi omics analyses reveal the interaction between colonic microbiota and host in min and yorkshire pigs |
| topic | biomarkers colonic microorganism Min pigs Multi‐omics Yorkshire pigs |
| url | https://doi.org/10.1002/aro2.39 |
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