Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice
Healthy brain development hinges on proper myelination, with disruption contributing to a wide array of neurological disorders. Immunohistochemical analysis of myelin basic protein (MBP) is a fundamental technique for investigating myelination and related disorders. However, despite decades of MBP r...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2025.1535745/full |
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| author | Siddhi S. Ozarkar Siddhi S. Ozarkar Ridthi K. R. Patel Ridthi K. R. Patel Tasmai Vulli Tasmai Vulli Carlee A. Friar Carlee A. Friar Alain C. Burette Alain C. Burette Benjamin D. Philpot Benjamin D. Philpot Benjamin D. Philpot |
| author_facet | Siddhi S. Ozarkar Siddhi S. Ozarkar Ridthi K. R. Patel Ridthi K. R. Patel Tasmai Vulli Tasmai Vulli Carlee A. Friar Carlee A. Friar Alain C. Burette Alain C. Burette Benjamin D. Philpot Benjamin D. Philpot Benjamin D. Philpot |
| author_sort | Siddhi S. Ozarkar |
| collection | DOAJ |
| description | Healthy brain development hinges on proper myelination, with disruption contributing to a wide array of neurological disorders. Immunohistochemical analysis of myelin basic protein (MBP) is a fundamental technique for investigating myelination and related disorders. However, despite decades of MBP research, detailed accounts of normal MBP progression in the developing mouse brain have been lacking. This study aims to address this gap by providing a detailed spatiotemporal account of MBP distribution across 13 developmental ages from postnatal day 2 to 60. We used an optimized immunohistochemistry protocol to overcome the challenges of myelin’s unique lipid-rich composition, enabling more consistent staining across diverse brain structures and developmental stages, offering a robust baseline for typical myelination patterns, and enabling comparisons with pathological models. To support and potentially accelerate research into myelination disorders, we have made >1,400 high-resolution micrographs accessible online under the Creative Commons license. |
| format | Article |
| id | doaj-art-d282ef67d86c49f4ab34efbbc691f7a7 |
| institution | DOAJ |
| issn | 1662-5129 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj-art-d282ef67d86c49f4ab34efbbc691f7a72025-08-20T03:02:06ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292025-03-011910.3389/fnana.2025.15357451535745Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J miceSiddhi S. Ozarkar0Siddhi S. Ozarkar1Ridthi K. R. Patel2Ridthi K. R. Patel3Tasmai Vulli4Tasmai Vulli5Carlee A. Friar6Carlee A. Friar7Alain C. Burette8Alain C. Burette9Benjamin D. Philpot10Benjamin D. Philpot11Benjamin D. Philpot12Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesCarolina Institute for Developmental Disabilities, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesHealthy brain development hinges on proper myelination, with disruption contributing to a wide array of neurological disorders. Immunohistochemical analysis of myelin basic protein (MBP) is a fundamental technique for investigating myelination and related disorders. However, despite decades of MBP research, detailed accounts of normal MBP progression in the developing mouse brain have been lacking. This study aims to address this gap by providing a detailed spatiotemporal account of MBP distribution across 13 developmental ages from postnatal day 2 to 60. We used an optimized immunohistochemistry protocol to overcome the challenges of myelin’s unique lipid-rich composition, enabling more consistent staining across diverse brain structures and developmental stages, offering a robust baseline for typical myelination patterns, and enabling comparisons with pathological models. To support and potentially accelerate research into myelination disorders, we have made >1,400 high-resolution micrographs accessible online under the Creative Commons license.https://www.frontiersin.org/articles/10.3389/fnana.2025.1535745/fullmyelinmyelin basic proteinpostnatal developmentwhite mattermyelin-associated glycoprotein |
| spellingShingle | Siddhi S. Ozarkar Siddhi S. Ozarkar Ridthi K. R. Patel Ridthi K. R. Patel Tasmai Vulli Tasmai Vulli Carlee A. Friar Carlee A. Friar Alain C. Burette Alain C. Burette Benjamin D. Philpot Benjamin D. Philpot Benjamin D. Philpot Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice Frontiers in Neuroanatomy myelin myelin basic protein postnatal development white matter myelin-associated glycoprotein |
| title | Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice |
| title_full | Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice |
| title_fullStr | Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice |
| title_full_unstemmed | Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice |
| title_short | Regional analysis of myelin basic protein across postnatal brain development of C57BL/6J mice |
| title_sort | regional analysis of myelin basic protein across postnatal brain development of c57bl 6j mice |
| topic | myelin myelin basic protein postnatal development white matter myelin-associated glycoprotein |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2025.1535745/full |
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