MicroRNA - biomarker of aggressiveness of choroidal melanoma
Almost 50 % of microRNAs (a family of small noncoding RNAs) are associated with the regions of the genome responsible for the development of tumors. These microRNAs play the role of oncogenes or tumor suppressor genes. In 2008, there were reports of the possibility of using microRNA as a predictive...
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Real Time Ltd
2022-03-01
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| Series: | Российский офтальмологический журнал |
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| Online Access: | https://roj.igb.ru/jour/article/view/880 |
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| author | A. F. Brovkina N. D. Tsybikova |
| author_facet | A. F. Brovkina N. D. Tsybikova |
| author_sort | A. F. Brovkina |
| collection | DOAJ |
| description | Almost 50 % of microRNAs (a family of small noncoding RNAs) are associated with the regions of the genome responsible for the development of tumors. These microRNAs play the role of oncogenes or tumor suppressor genes. In 2008, there were reports of the possibility of using microRNA as a predictive biomarker of the metastatic risk of uveal melanoma. Initially, microRNAs were investigated in melanoma samples; later, the possibility of using blood plasma for these purposes was shown.Purpose: to study the character of expression of miRNA- 146a, miRNA-155, miRNA-223, miRNA-126, miRNA-27b in the blood plasma of patients with choroidal melanoma (CM) and determine their significance in predicting possible hematogenous metastases. Material and methods. The study included 84 patients with CM aged 35–86 (ave 63.4 ± 1.2 yrs). The thickness of the CM varied in the range of 0.77–17.19 mm (ave 7.21 ± 0.43 mm). The control group consisted of 28 volunteers aged 45-78 (62.90 ± 1.42 yrs). MicroRNA expression levels were determined by quantitative PCR.Results. An increase in the expression level of miRNA-155, miRNA-146a, miRNA-126, miRNA-223, and miRNA-27b in blood plasma in all 84 patients with CM was revealed.Conclusion. The study of miRNA levels (miRNA-146, miRNA-155, miRNA-223, miRNA-126 and miRNA-27b) in the blood plasma of patients with CM can be used both to confirm the diagnosis of CM in difficult diagnostic cases and to determine the aggressiveness of the course tumor and prediction of metastasis. |
| format | Article |
| id | doaj-art-d281ec6339ab41189fd2294790d2e2d3 |
| institution | DOAJ |
| issn | 2072-0076 2587-5760 |
| language | Russian |
| publishDate | 2022-03-01 |
| publisher | Real Time Ltd |
| record_format | Article |
| series | Российский офтальмологический журнал |
| spelling | doaj-art-d281ec6339ab41189fd2294790d2e2d32025-08-20T03:19:24ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602022-03-0115171210.21516/2072-0076-2022-15-1-7-12417MicroRNA - biomarker of aggressiveness of choroidal melanomaA. F. Brovkina0N. D. Tsybikova1Russian Medical Academy of Continuous Professional Education; Botkin HospitalRussian Medical Academy of Continuous Professional Education; Botkin HospitalAlmost 50 % of microRNAs (a family of small noncoding RNAs) are associated with the regions of the genome responsible for the development of tumors. These microRNAs play the role of oncogenes or tumor suppressor genes. In 2008, there were reports of the possibility of using microRNA as a predictive biomarker of the metastatic risk of uveal melanoma. Initially, microRNAs were investigated in melanoma samples; later, the possibility of using blood plasma for these purposes was shown.Purpose: to study the character of expression of miRNA- 146a, miRNA-155, miRNA-223, miRNA-126, miRNA-27b in the blood plasma of patients with choroidal melanoma (CM) and determine their significance in predicting possible hematogenous metastases. Material and methods. The study included 84 patients with CM aged 35–86 (ave 63.4 ± 1.2 yrs). The thickness of the CM varied in the range of 0.77–17.19 mm (ave 7.21 ± 0.43 mm). The control group consisted of 28 volunteers aged 45-78 (62.90 ± 1.42 yrs). MicroRNA expression levels were determined by quantitative PCR.Results. An increase in the expression level of miRNA-155, miRNA-146a, miRNA-126, miRNA-223, and miRNA-27b in blood plasma in all 84 patients with CM was revealed.Conclusion. The study of miRNA levels (miRNA-146, miRNA-155, miRNA-223, miRNA-126 and miRNA-27b) in the blood plasma of patients with CM can be used both to confirm the diagnosis of CM in difficult diagnostic cases and to determine the aggressiveness of the course tumor and prediction of metastasis.https://roj.igb.ru/jour/article/view/880choroidal melanomamirna-146amirna-155mirna-223mirna-126mirna-27bbiomarker |
| spellingShingle | A. F. Brovkina N. D. Tsybikova MicroRNA - biomarker of aggressiveness of choroidal melanoma Российский офтальмологический журнал choroidal melanoma mirna-146a mirna-155 mirna-223 mirna-126 mirna-27b biomarker |
| title | MicroRNA - biomarker of aggressiveness of choroidal melanoma |
| title_full | MicroRNA - biomarker of aggressiveness of choroidal melanoma |
| title_fullStr | MicroRNA - biomarker of aggressiveness of choroidal melanoma |
| title_full_unstemmed | MicroRNA - biomarker of aggressiveness of choroidal melanoma |
| title_short | MicroRNA - biomarker of aggressiveness of choroidal melanoma |
| title_sort | microrna biomarker of aggressiveness of choroidal melanoma |
| topic | choroidal melanoma mirna-146a mirna-155 mirna-223 mirna-126 mirna-27b biomarker |
| url | https://roj.igb.ru/jour/article/view/880 |
| work_keys_str_mv | AT afbrovkina micrornabiomarkerofaggressivenessofchoroidalmelanoma AT ndtsybikova micrornabiomarkerofaggressivenessofchoroidalmelanoma |