Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis
IntroductionAtmospheric CO2 elevation significantly impacts plant carbon metabolism, yet accurate quantification of respiratory parameters—photorespiration rate (Rp) and mitochondrial respiration rate in the light (Rd)—under varying CO2 remains challenging. Current CO2-response models exhibit limita...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1455533/full |
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| author | Zhengwen Niu Zi-Wu-Yin Ye Qi Huang Chunju Peng Huajing Kang |
| author_facet | Zhengwen Niu Zi-Wu-Yin Ye Qi Huang Chunju Peng Huajing Kang |
| author_sort | Zhengwen Niu |
| collection | DOAJ |
| description | IntroductionAtmospheric CO2 elevation significantly impacts plant carbon metabolism, yet accurate quantification of respiratory parameters—photorespiration rate (Rp) and mitochondrial respiration rate in the light (Rd)—under varying CO2 remains challenging. Current CO2-response models exhibit limitations in estimating these parameters, hindering predictions of crop responses under future climate scenarios.MethodsLow-oxygen treatments and gas exchange measurements, calculating CO2 recovery/inhibition ratio in of wheat (Triticum aestivum L.) and bean (Glycine max L.) were employed to elucidate the biological significance and interrelationships of Rp and Rd. Model-derived estimates of Rp and Rd were compared with measured values to assess the accuracy of three CO2-response models (biochemical, rectangular hyperbola, modified rectangular hyperbola). Furthermore, the effects of ambient CO2 concentration (0~1200 μmol·mol-1) on the measured Rp and Rd were quantified through polynomial regression.ResultsThe A/Ca model achieved superior fitting performance over the A/Ci model. However, significant disparities persisted between A/Ca-derived Rp/Rd estimates and measurements (p < 0.05). CO2 concentration exhibited dose-dependent regulation of respiratory fluxes: Rp-measured ranged from 4.923 ± 0.171 to 12.307 ± 1.033 μmol (CO2) m-2 s-1 (wheat) and 4.686 ± 0.274 to 11.673 ± 2.054 μmol (CO2) m-2 s⁻ ¹ (bean), while Rd-measured varied from 0.618 ± 0.131 to 3.021 ± 0.063 μmol (CO2) m-2 s-1 (wheat) and 0.492 ± 0.069 to 2.323 ± 0.312 μmol (CO2) m-2 s-1 (bean). Polynomial regression revealed strong non-linear correlations between CO2 concentrations and respiratory parameters (R² > 0.891, p < 0.05; except bean Rp-Ca: R² = 0.797). Species-specific CO2 thresholds governed peak Rp (600 μmol·mol-1 for wheat vs. 1,000 μmol·mol-1 for bean) and Rd (400 μmol·mol-1 for wheat vs. 200 μmol·mol-1 for bean).DiscussionThese findings expose critical limitations in current respiratory parameter quantification methods and challenge linear assumptions of CO2-respiration relationships. They establish a critical framework for refining photosynthetic models by incorporating CO2-responsive respiratory mechanisms. The identified non-linear regulatory patterns and model limitations provide actionable insights for advancing carbon metabolism theory and optimizing crop carbon assimilation strategies under rising atmospheric CO2, with implications for climate-resilient agricultural practices. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-08-01 |
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| spelling | doaj-art-d28115338b7d4e97bd844de2b51b13fb2025-08-26T05:28:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.14555331455533Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesisZhengwen Niu0Zi-Wu-Yin Ye1Qi Huang2Chunju Peng3Huajing Kang4Wenzhou Key Laboratory of Agricultural & Forestry Carbon Sequestration and Tea Resource Development, Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang, ChinaCollege of International Studies, Guangdong Baiyun University, Guangzhou, Guangdong, ChinaWenzhou Key Laboratory of Agricultural & Forestry Carbon Sequestration and Tea Resource Development, Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang, ChinaWenzhou Key Laboratory of Agricultural & Forestry Carbon Sequestration and Tea Resource Development, Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang, ChinaWenzhou Key Laboratory of Agricultural & Forestry Carbon Sequestration and Tea Resource Development, Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang, ChinaIntroductionAtmospheric CO2 elevation significantly impacts plant carbon metabolism, yet accurate quantification of respiratory parameters—photorespiration rate (Rp) and mitochondrial respiration rate in the light (Rd)—under varying CO2 remains challenging. Current CO2-response models exhibit limitations in estimating these parameters, hindering predictions of crop responses under future climate scenarios.MethodsLow-oxygen treatments and gas exchange measurements, calculating CO2 recovery/inhibition ratio in of wheat (Triticum aestivum L.) and bean (Glycine max L.) were employed to elucidate the biological significance and interrelationships of Rp and Rd. Model-derived estimates of Rp and Rd were compared with measured values to assess the accuracy of three CO2-response models (biochemical, rectangular hyperbola, modified rectangular hyperbola). Furthermore, the effects of ambient CO2 concentration (0~1200 μmol·mol-1) on the measured Rp and Rd were quantified through polynomial regression.ResultsThe A/Ca model achieved superior fitting performance over the A/Ci model. However, significant disparities persisted between A/Ca-derived Rp/Rd estimates and measurements (p < 0.05). CO2 concentration exhibited dose-dependent regulation of respiratory fluxes: Rp-measured ranged from 4.923 ± 0.171 to 12.307 ± 1.033 μmol (CO2) m-2 s-1 (wheat) and 4.686 ± 0.274 to 11.673 ± 2.054 μmol (CO2) m-2 s⁻ ¹ (bean), while Rd-measured varied from 0.618 ± 0.131 to 3.021 ± 0.063 μmol (CO2) m-2 s-1 (wheat) and 0.492 ± 0.069 to 2.323 ± 0.312 μmol (CO2) m-2 s-1 (bean). Polynomial regression revealed strong non-linear correlations between CO2 concentrations and respiratory parameters (R² > 0.891, p < 0.05; except bean Rp-Ca: R² = 0.797). Species-specific CO2 thresholds governed peak Rp (600 μmol·mol-1 for wheat vs. 1,000 μmol·mol-1 for bean) and Rd (400 μmol·mol-1 for wheat vs. 200 μmol·mol-1 for bean).DiscussionThese findings expose critical limitations in current respiratory parameter quantification methods and challenge linear assumptions of CO2-respiration relationships. They establish a critical framework for refining photosynthetic models by incorporating CO2-responsive respiratory mechanisms. The identified non-linear regulatory patterns and model limitations provide actionable insights for advancing carbon metabolism theory and optimizing crop carbon assimilation strategies under rising atmospheric CO2, with implications for climate-resilient agricultural practices.https://www.frontiersin.org/articles/10.3389/fpls.2025.1455533/fullCO2 concentrationCO2 recoverymitochondrial respiration ratephotorespirationglobal change |
| spellingShingle | Zhengwen Niu Zi-Wu-Yin Ye Qi Huang Chunju Peng Huajing Kang Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis Frontiers in Plant Science CO2 concentration CO2 recovery mitochondrial respiration rate photorespiration global change |
| title | Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis |
| title_full | Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis |
| title_fullStr | Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis |
| title_full_unstemmed | Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis |
| title_short | Accuracy of photorespiration and mitochondrial respiration in the light fitted by CO2 response model for photosynthesis |
| title_sort | accuracy of photorespiration and mitochondrial respiration in the light fitted by co2 response model for photosynthesis |
| topic | CO2 concentration CO2 recovery mitochondrial respiration rate photorespiration global change |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1455533/full |
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