Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro
<i>Vaccinium uliginosum</i> L. (VU), rich in polyphenols, is an important wild berry resource primarily distributed in extremely cold regions. However, the detailed composition of <i>Vaccinium uliginosum</i> L. polyphenols (VUPs) has not been reported, which limits the develo...
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2024-10-01
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| author | Ying Zhang Wenjiang Dong Manjun Zhao Jiyue Zhang Li Li Yan Ma Xianjun Meng Yuehua Wang |
| author_facet | Ying Zhang Wenjiang Dong Manjun Zhao Jiyue Zhang Li Li Yan Ma Xianjun Meng Yuehua Wang |
| author_sort | Ying Zhang |
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| description | <i>Vaccinium uliginosum</i> L. (VU), rich in polyphenols, is an important wild berry resource primarily distributed in extremely cold regions. However, the detailed composition of <i>Vaccinium uliginosum</i> L. polyphenols (VUPs) has not been reported, which limits the development and utilization of VU. In this study, VU-free polyphenols (VUFPs) and VU-bound polyphenols (VUBPs) were, respectively, extracted using an ultrasonic, complex enzyme and alkali extraction method; the compositions were identified using ultra-performance liquid chromatography–electrospray ionization mass spectrometry, and lipid-lowering activity in vitro was evaluated. The results showed that 885 polyphenols and 47 anthocyanins were detected in the VUFPs and VUBPs, and 30 anthocyanin monomers were firstly detected in VU. Compared with the model group, the accumulation of lipid droplets and the total cholesterol and triglyceride contents in the high-concentration VUP group reduced by 36.95%, 65.82%, and 62.43%, respectively, and liver damage was also alleviated. It was also found that VUP can regulate the level of <i>Asialoglycoprotein receptor</i> 1, a new target for lipid lowering. In summary, this study provides a detailed report on VUP for the first time, confirming that VUP has lipid-lowering potential in vitro. These findings suggest new strategies and theoretical support for the development and utilization of VU, especially in the field of functional foods. |
| format | Article |
| id | doaj-art-d258d0de1498431db36c50a0ea7f12ab |
| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-d258d0de1498431db36c50a0ea7f12ab2025-08-20T02:13:14ZengMDPI AGFoods2304-81582024-10-011321343810.3390/foods13213438Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In VitroYing Zhang0Wenjiang Dong1Manjun Zhao2Jiyue Zhang3Li Li4Yan Ma5Xianjun Meng6Yuehua Wang7College of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang 110866, ChinaSpice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, National Center of Important Tropical Crops Engineering and Technology Research, Wanning 571533, ChinaCollege of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang 110866, ChinaSpice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, National Center of Important Tropical Crops Engineering and Technology Research, Wanning 571533, ChinaCollege of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang 110866, ChinaCenter of Experiment Teaching, Shenyang Normal University, Shenyang 110034, ChinaCollege of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Dongling Road 120, Shenhe District, Shenyang 110866, China<i>Vaccinium uliginosum</i> L. (VU), rich in polyphenols, is an important wild berry resource primarily distributed in extremely cold regions. However, the detailed composition of <i>Vaccinium uliginosum</i> L. polyphenols (VUPs) has not been reported, which limits the development and utilization of VU. In this study, VU-free polyphenols (VUFPs) and VU-bound polyphenols (VUBPs) were, respectively, extracted using an ultrasonic, complex enzyme and alkali extraction method; the compositions were identified using ultra-performance liquid chromatography–electrospray ionization mass spectrometry, and lipid-lowering activity in vitro was evaluated. The results showed that 885 polyphenols and 47 anthocyanins were detected in the VUFPs and VUBPs, and 30 anthocyanin monomers were firstly detected in VU. Compared with the model group, the accumulation of lipid droplets and the total cholesterol and triglyceride contents in the high-concentration VUP group reduced by 36.95%, 65.82%, and 62.43%, respectively, and liver damage was also alleviated. It was also found that VUP can regulate the level of <i>Asialoglycoprotein receptor</i> 1, a new target for lipid lowering. In summary, this study provides a detailed report on VUP for the first time, confirming that VUP has lipid-lowering potential in vitro. These findings suggest new strategies and theoretical support for the development and utilization of VU, especially in the field of functional foods.https://www.mdpi.com/2304-8158/13/21/3438<i>Vaccinium uliginosum</i> L.polyphenolanthocyaninslipid loweringin vitro |
| spellingShingle | Ying Zhang Wenjiang Dong Manjun Zhao Jiyue Zhang Li Li Yan Ma Xianjun Meng Yuehua Wang Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro Foods <i>Vaccinium uliginosum</i> L. polyphenol anthocyanins lipid lowering in vitro |
| title | Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro |
| title_full | Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro |
| title_fullStr | Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro |
| title_full_unstemmed | Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro |
| title_short | Identification and Analysis of Phenolic Compounds in <i>Vaccinium uliginosum</i> L. and Its Lipid-Lowering Activity In Vitro |
| title_sort | identification and analysis of phenolic compounds in i vaccinium uliginosum i l and its lipid lowering activity in vitro |
| topic | <i>Vaccinium uliginosum</i> L. polyphenol anthocyanins lipid lowering in vitro |
| url | https://www.mdpi.com/2304-8158/13/21/3438 |
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