Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation
Ligustrum japonicum fruits have been used as a part of traditional medicinal practices and supplements in Korea and Japan. It has been reported to possess various bioactivities, but its antiosteoporotic potential and active substances have not been reported yet. The present study followed an ALP act...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8851884 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849473462456811520 |
|---|---|
| author | Jung Hwan Oh Fatih Karadeniz Jung Im Lee Youngwan Seo Chang-Suk Kong |
| author_facet | Jung Hwan Oh Fatih Karadeniz Jung Im Lee Youngwan Seo Chang-Suk Kong |
| author_sort | Jung Hwan Oh |
| collection | DOAJ |
| description | Ligustrum japonicum fruits have been used as a part of traditional medicinal practices and supplements in Korea and Japan. It has been reported to possess various bioactivities, but its antiosteoporotic potential and active substances have not been reported yet. The present study followed an ALP activity and lipid accumulation-guided screening of L. japonicum fruits for antiosteoporotic compounds and isolated salidroside as an active compound. Antiosteoporotic effects of L. japonicum fruits and salidroside were examined in mesenchymal stromal cells by their ability to enhance osteoblast formation by increased ALP activity and osteogenic marker gene expression while suppressing adipogenesis by inhibition of lipid accumulation and adipocyte marker gene expressions. Results showed that salidroside was able to enhance osteoblast differentiation via Wnt/BMP signaling pathway overactivation and suppress the PPARγ-mediated adipocyte differentiation, both through the MAPK pathway. In conclusion, L. japonicum fruits were suggested to possess antiosteoporotic activities and to be a source of antiosteoporotic substances such as salidroside. |
| format | Article |
| id | doaj-art-d257a9efaea84cae96749e00969d7938 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d257a9efaea84cae96749e00969d79382025-08-20T03:24:07ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/88518848851884Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast DifferentiationJung Hwan Oh0Fatih Karadeniz1Jung Im Lee2Youngwan Seo3Chang-Suk Kong4Marine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaMarine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaMarine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaDivision of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Republic of KoreaMarine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaLigustrum japonicum fruits have been used as a part of traditional medicinal practices and supplements in Korea and Japan. It has been reported to possess various bioactivities, but its antiosteoporotic potential and active substances have not been reported yet. The present study followed an ALP activity and lipid accumulation-guided screening of L. japonicum fruits for antiosteoporotic compounds and isolated salidroside as an active compound. Antiosteoporotic effects of L. japonicum fruits and salidroside were examined in mesenchymal stromal cells by their ability to enhance osteoblast formation by increased ALP activity and osteogenic marker gene expression while suppressing adipogenesis by inhibition of lipid accumulation and adipocyte marker gene expressions. Results showed that salidroside was able to enhance osteoblast differentiation via Wnt/BMP signaling pathway overactivation and suppress the PPARγ-mediated adipocyte differentiation, both through the MAPK pathway. In conclusion, L. japonicum fruits were suggested to possess antiosteoporotic activities and to be a source of antiosteoporotic substances such as salidroside.http://dx.doi.org/10.1155/2021/8851884 |
| spellingShingle | Jung Hwan Oh Fatih Karadeniz Jung Im Lee Youngwan Seo Chang-Suk Kong Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation Stem Cells International |
| title | Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation |
| title_full | Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation |
| title_fullStr | Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation |
| title_full_unstemmed | Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation |
| title_short | Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation |
| title_sort | ligustrum japonicum thunb fruits exert antiosteoporotic properties in bone marrow derived mesenchymal stromal cells via regulation of adipocyte and osteoblast differentiation |
| url | http://dx.doi.org/10.1155/2021/8851884 |
| work_keys_str_mv | AT junghwanoh ligustrumjaponicumthunbfruitsexertantiosteoporoticpropertiesinbonemarrowderivedmesenchymalstromalcellsviaregulationofadipocyteandosteoblastdifferentiation AT fatihkaradeniz ligustrumjaponicumthunbfruitsexertantiosteoporoticpropertiesinbonemarrowderivedmesenchymalstromalcellsviaregulationofadipocyteandosteoblastdifferentiation AT jungimlee ligustrumjaponicumthunbfruitsexertantiosteoporoticpropertiesinbonemarrowderivedmesenchymalstromalcellsviaregulationofadipocyteandosteoblastdifferentiation AT youngwanseo ligustrumjaponicumthunbfruitsexertantiosteoporoticpropertiesinbonemarrowderivedmesenchymalstromalcellsviaregulationofadipocyteandosteoblastdifferentiation AT changsukkong ligustrumjaponicumthunbfruitsexertantiosteoporoticpropertiesinbonemarrowderivedmesenchymalstromalcellsviaregulationofadipocyteandosteoblastdifferentiation |