Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression
Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/3893691 |
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| author | Xufeng Fu Yaping Yan Shanshan Li Junfeng Wang Bin Jiang Hong Wang Yanchao Duan Tao Tan Fei Gao Desheng Gong Yuyu Niu Weizhi Ji Bingrong Zheng Wei Si |
| author_facet | Xufeng Fu Yaping Yan Shanshan Li Junfeng Wang Bin Jiang Hong Wang Yanchao Duan Tao Tan Fei Gao Desheng Gong Yuyu Niu Weizhi Ji Bingrong Zheng Wei Si |
| author_sort | Xufeng Fu |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservation remain unknown. In the present study, the viability, immunophenotype of cell surface markers, proliferation, differentiation potency, and global gene expression of rhesus macaque bone marrow-derived MSCs vitrified using DMSO and EG were studied. The results showed that vitrification did not affect the morphology, surface markers, and differentiation of the MSCs, and compared to DMSO, EG better protected cell viability and proliferation. Most importantly, vitrification resulted in changes in a large number of transcripts of MSCs either preserved using DMSO or EG. This report is the first to examine the effects of DMSO and EG on global gene expression in stem cells. These results will be beneficial to understanding the biological process involved in MSC vitrification and will contribute to improving cryopreservation protocols that maintain transcriptomic identity with high cryosurvival for preclinical research and clinical long-term storage. |
| format | Article |
| id | doaj-art-d2510d1f789f4441a9ff3e55f4e3fb82 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d2510d1f789f4441a9ff3e55f4e3fb822025-08-20T02:19:43ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/38936913893691Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene ExpressionXufeng Fu0Yaping Yan1Shanshan Li2Junfeng Wang3Bin Jiang4Hong Wang5Yanchao Duan6Tao Tan7Fei Gao8Desheng Gong9Yuyu Niu10Weizhi Ji11Bingrong Zheng12Wei Si13Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaDepartment of Hepatic and Bile Duct Surgery, The First People’s Hospital of Yunnan Province, Kunming 650032, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaSchool of Medicine, Yunnan University, Kunming 650091, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaMesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservation remain unknown. In the present study, the viability, immunophenotype of cell surface markers, proliferation, differentiation potency, and global gene expression of rhesus macaque bone marrow-derived MSCs vitrified using DMSO and EG were studied. The results showed that vitrification did not affect the morphology, surface markers, and differentiation of the MSCs, and compared to DMSO, EG better protected cell viability and proliferation. Most importantly, vitrification resulted in changes in a large number of transcripts of MSCs either preserved using DMSO or EG. This report is the first to examine the effects of DMSO and EG on global gene expression in stem cells. These results will be beneficial to understanding the biological process involved in MSC vitrification and will contribute to improving cryopreservation protocols that maintain transcriptomic identity with high cryosurvival for preclinical research and clinical long-term storage.http://dx.doi.org/10.1155/2017/3893691 |
| spellingShingle | Xufeng Fu Yaping Yan Shanshan Li Junfeng Wang Bin Jiang Hong Wang Yanchao Duan Tao Tan Fei Gao Desheng Gong Yuyu Niu Weizhi Ji Bingrong Zheng Wei Si Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression Stem Cells International |
| title | Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression |
| title_full | Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression |
| title_fullStr | Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression |
| title_full_unstemmed | Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression |
| title_short | Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression |
| title_sort | vitrification of rhesus macaque mesenchymal stem cells and the effects on global gene expression |
| url | http://dx.doi.org/10.1155/2017/3893691 |
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