Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea
In this study, the effect of Bangia fusco-purpurea polysaccharide (BFP) on Escherichia coli adhesion to Caco-2 monolayers and its potential mechanism was studied using an in vitro human colon adenocarcinoma cell (Caco-2) monolayer model. The effect of BFP on the adhesion of E. coli to Caco-2 monolay...
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China Food Publishing Company
2024-09-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-002.pdf |
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| author | DUAN Shushu, ZHENG Mingjing, HONG Tao, ZHU Yanbing, NI Hui, JIANG Zedong |
| author_facet | DUAN Shushu, ZHENG Mingjing, HONG Tao, ZHU Yanbing, NI Hui, JIANG Zedong |
| author_sort | DUAN Shushu, ZHENG Mingjing, HONG Tao, ZHU Yanbing, NI Hui, JIANG Zedong |
| collection | DOAJ |
| description | In this study, the effect of Bangia fusco-purpurea polysaccharide (BFP) on Escherichia coli adhesion to Caco-2 monolayers and its potential mechanism was studied using an in vitro human colon adenocarcinoma cell (Caco-2) monolayer model. The effect of BFP on the adhesion of E. coli to Caco-2 monolayers was analyzed by fluorescence labeling with carboxyfluorescein diacetate and succinimidyl ester (CFDA-SE), and its effect on the gene expression of integrin β1 in Caco-2 cells, the adhesin FimH in E. coli, and inflammatory factors (IL-1β, IL-8 and TNF-α) induced by E. coli adhesion to Caco-2 cells and the tight junction proteins zonula occludens 1 (ZO-1) and occludin were analyzed using real-time polymerase chain reaction (real-time PCR). Also, the protein expression of ZO-1 and occludin in Caco-2 monolayers was detected using Western Blot analysis. Our results showed that BFP at concentrations of 400–800 μg/mL significantly inhibited the adhesion of E. coli to Caco-2 monolayers mainly by down-regulating the gene expression of integrin β1 in Caco-2 cells and FimH in E. coli. In addition, BFP significantly inhibited the up-regulation of inflammatory cytokine gene expression and the down-regulation of ZO-1 and occludin protein and gene expression induced by in Caco-2 cells induced by E. coli and its culture supernatant. In conclusion, BFP inhibited the adhesion of E. coli to Caco-2 cell monolayers, which will lay a scientific basis for the development of novel antimicrobial products and the high-value utilization and deep processing of BFP. |
| format | Article |
| id | doaj-art-d24efbc8b84d417cb25b7ae5e6230c9d |
| institution | OA Journals |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-d24efbc8b84d417cb25b7ae5e6230c9d2025-08-20T01:50:45ZengChina Food Publishing CompanyShipin Kexue1002-66302024-09-01451791610.7506/spkx1002-6630-20230719-204Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpureaDUAN Shushu, ZHENG Mingjing, HONG Tao, ZHU Yanbing, NI Hui, JIANG Zedong0(1. College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; 3. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China)In this study, the effect of Bangia fusco-purpurea polysaccharide (BFP) on Escherichia coli adhesion to Caco-2 monolayers and its potential mechanism was studied using an in vitro human colon adenocarcinoma cell (Caco-2) monolayer model. The effect of BFP on the adhesion of E. coli to Caco-2 monolayers was analyzed by fluorescence labeling with carboxyfluorescein diacetate and succinimidyl ester (CFDA-SE), and its effect on the gene expression of integrin β1 in Caco-2 cells, the adhesin FimH in E. coli, and inflammatory factors (IL-1β, IL-8 and TNF-α) induced by E. coli adhesion to Caco-2 cells and the tight junction proteins zonula occludens 1 (ZO-1) and occludin were analyzed using real-time polymerase chain reaction (real-time PCR). Also, the protein expression of ZO-1 and occludin in Caco-2 monolayers was detected using Western Blot analysis. Our results showed that BFP at concentrations of 400–800 μg/mL significantly inhibited the adhesion of E. coli to Caco-2 monolayers mainly by down-regulating the gene expression of integrin β1 in Caco-2 cells and FimH in E. coli. In addition, BFP significantly inhibited the up-regulation of inflammatory cytokine gene expression and the down-regulation of ZO-1 and occludin protein and gene expression induced by in Caco-2 cells induced by E. coli and its culture supernatant. In conclusion, BFP inhibited the adhesion of E. coli to Caco-2 cell monolayers, which will lay a scientific basis for the development of novel antimicrobial products and the high-value utilization and deep processing of BFP.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-002.pdfbangia fusco-purpurea polysaccharide; escherichia coli; caco-2 cells; bacterial adhesion; intestinal homeostasis |
| spellingShingle | DUAN Shushu, ZHENG Mingjing, HONG Tao, ZHU Yanbing, NI Hui, JIANG Zedong Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea Shipin Kexue bangia fusco-purpurea polysaccharide; escherichia coli; caco-2 cells; bacterial adhesion; intestinal homeostasis |
| title | Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea |
| title_full | Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea |
| title_fullStr | Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea |
| title_full_unstemmed | Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea |
| title_short | Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea |
| title_sort | inhibitory mechanism of escherichia coli adhesion to caco 2 monolayers by polysaccharides from bangia fusco purpurea |
| topic | bangia fusco-purpurea polysaccharide; escherichia coli; caco-2 cells; bacterial adhesion; intestinal homeostasis |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-17-002.pdf |
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