Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction
Quantification of mRNA expression levels using real-time reverse transcription PCR (RT-PCR) is increasingly used to validate results of DNA microarrays or GeneChips®. It requires an improved method that is more robust and more suitable for high-throughput measurements. In this report, we compare a u...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/05382RR05 |
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