Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage
Abstract Although freeze-dried (FD) sperm can be stored at room temperature, making it cheaper and safer to store and transport, freeze-drying can damage the sperm’s DNA, which can decrease the birth rate compared to frozen sperm. In this study, we examined whether adding monosodium glutamate (MSG),...
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| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-16635-9 |
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| author | Natsuki Ushigome Meina Miyashita Daiyu Ito Sayaka Wakayama Teruhiko Wakayama |
| author_facet | Natsuki Ushigome Meina Miyashita Daiyu Ito Sayaka Wakayama Teruhiko Wakayama |
| author_sort | Natsuki Ushigome |
| collection | DOAJ |
| description | Abstract Although freeze-dried (FD) sperm can be stored at room temperature, making it cheaper and safer to store and transport, freeze-drying can damage the sperm’s DNA, which can decrease the birth rate compared to frozen sperm. In this study, we examined whether adding monosodium glutamate (MSG), a compound used to dry and preserve yeast, to the drying medium could improve the quality of mouse FD sperm. FD sperm stored using MSG showed lower morphological and DNA damage, and the birth rate of embryos fertilized using those sperm improved to a similar level (50%) to that obtained with frozen-thawed sperm. The offspring showed normal growth and reproductive performance. Similar results were obtained for embryos derived from other mouse strains, even using FD sperm stored for 3 months at room temperature. These results suggest that MSG can protect not only microorganisms but also the DNA of mammalian FD sperm and improve the birth rate to a practical level. This method is not only inexpensive and safer than cryopreservation using liquid nitrogen but has expansion potential to maintain a large number of genetically modified mouse strains. |
| format | Article |
| id | doaj-art-d2474e2abc1e4a599149bedd2f592696 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d2474e2abc1e4a599149bedd2f5926962025-08-24T11:28:01ZengNature PortfolioScientific Reports2045-23222025-08-0115111210.1038/s41598-025-16635-9Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storageNatsuki Ushigome0Meina Miyashita1Daiyu Ito2Sayaka Wakayama3Teruhiko Wakayama4Faculty of Life and Environmental Science, University of YamanashiFaculty of Life and Environmental Science, University of YamanashiFaculty of Life and Environmental Science, University of YamanashiAdvanced Biotechnology Center, University of YamanashiAdvanced Biotechnology Center, University of YamanashiAbstract Although freeze-dried (FD) sperm can be stored at room temperature, making it cheaper and safer to store and transport, freeze-drying can damage the sperm’s DNA, which can decrease the birth rate compared to frozen sperm. In this study, we examined whether adding monosodium glutamate (MSG), a compound used to dry and preserve yeast, to the drying medium could improve the quality of mouse FD sperm. FD sperm stored using MSG showed lower morphological and DNA damage, and the birth rate of embryos fertilized using those sperm improved to a similar level (50%) to that obtained with frozen-thawed sperm. The offspring showed normal growth and reproductive performance. Similar results were obtained for embryos derived from other mouse strains, even using FD sperm stored for 3 months at room temperature. These results suggest that MSG can protect not only microorganisms but also the DNA of mammalian FD sperm and improve the birth rate to a practical level. This method is not only inexpensive and safer than cryopreservation using liquid nitrogen but has expansion potential to maintain a large number of genetically modified mouse strains.https://doi.org/10.1038/s41598-025-16635-9Monosodium glutamateFreeze-drySpermatozoaPreservationRoom temperature |
| spellingShingle | Natsuki Ushigome Meina Miyashita Daiyu Ito Sayaka Wakayama Teruhiko Wakayama Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage Scientific Reports Monosodium glutamate Freeze-dry Spermatozoa Preservation Room temperature |
| title | Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage |
| title_full | Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage |
| title_fullStr | Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage |
| title_full_unstemmed | Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage |
| title_short | Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage |
| title_sort | monosodium glutamate enhances freeze dried mouse sperm quality during room temperature storage |
| topic | Monosodium glutamate Freeze-dry Spermatozoa Preservation Room temperature |
| url | https://doi.org/10.1038/s41598-025-16635-9 |
| work_keys_str_mv | AT natsukiushigome monosodiumglutamateenhancesfreezedriedmousespermqualityduringroomtemperaturestorage AT meinamiyashita monosodiumglutamateenhancesfreezedriedmousespermqualityduringroomtemperaturestorage AT daiyuito monosodiumglutamateenhancesfreezedriedmousespermqualityduringroomtemperaturestorage AT sayakawakayama monosodiumglutamateenhancesfreezedriedmousespermqualityduringroomtemperaturestorage AT teruhikowakayama monosodiumglutamateenhancesfreezedriedmousespermqualityduringroomtemperaturestorage |