The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma

The paper provides a clinical description and analysis of two melanoma cases with mutations in the BRAF and NRAS gene within one tumor. Until recently, it was believed that mutations in the BRAF and NRAS genes are exclusive and do not occur within the same tumor. Later it was revealed that these mut...

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Main Authors: M. B. Aksenenko, A. S. Averchuk, T. G. Ruksha
Format: Article
Language:English
Published: Siberian State Medical University (Tomsk) 2019-10-01
Series:Бюллетень сибирской медицины
Subjects:
Online Access:https://bulletin.ssmu.ru/jour/article/view/2420
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author M. B. Aksenenko
A. S. Averchuk
T. G. Ruksha
author_facet M. B. Aksenenko
A. S. Averchuk
T. G. Ruksha
author_sort M. B. Aksenenko
collection DOAJ
description The paper provides a clinical description and analysis of two melanoma cases with mutations in the BRAF and NRAS gene within one tumor. Until recently, it was believed that mutations in the BRAF and NRAS genes are exclusive and do not occur within the same tumor. Later it was revealed that these mutations can exist within one tumor, but this is still quite a rare phenomenon. Molecular genetic analysis of the tumor obtained from both patients showed a mutation in the 15 exon of the BRAF gene c.1799T>A (V600E) and exon 2 of the NRAS p.G13S gene (p.37 G>A). The expression level of non-mutant NRAS protein was different in both cases. In conclusion, so in one patient, despite the presence of a mutation in the NRAS gene, the level of expression of the non-mutant protein was absent, which can be related to possible posttranscriptional disorders in the regulation of gene expression.
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spelling doaj-art-d244a1c9874146e0952e6ddd1d50c64f2025-08-20T03:02:03ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842019-10-0118322623110.20538/1682-0363-2019-3-226-2311556The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanomaM. B. Aksenenko0A. S. Averchuk1T. G. Ruksha2Krasnoyarsk State Medical University n.a. prof. V.F. Voyno-Yasenetsky (KrasSMU n.a. prof. V.F. Voyno-Yasenetsky)Krasnoyarsk State Medical University n.a. prof. V.F. Voyno-Yasenetsky (KrasSMU n.a. prof. V.F. Voyno-Yasenetsky)Krasnoyarsk State Medical University n.a. prof. V.F. Voyno-Yasenetsky (KrasSMU n.a. prof. V.F. Voyno-Yasenetsky)The paper provides a clinical description and analysis of two melanoma cases with mutations in the BRAF and NRAS gene within one tumor. Until recently, it was believed that mutations in the BRAF and NRAS genes are exclusive and do not occur within the same tumor. Later it was revealed that these mutations can exist within one tumor, but this is still quite a rare phenomenon. Molecular genetic analysis of the tumor obtained from both patients showed a mutation in the 15 exon of the BRAF gene c.1799T>A (V600E) and exon 2 of the NRAS p.G13S gene (p.37 G>A). The expression level of non-mutant NRAS protein was different in both cases. In conclusion, so in one patient, despite the presence of a mutation in the NRAS gene, the level of expression of the non-mutant protein was absent, which can be related to possible posttranscriptional disorders in the regulation of gene expression.https://bulletin.ssmu.ru/jour/article/view/2420melanoma of the skinbrafnrasmutations
spellingShingle M. B. Aksenenko
A. S. Averchuk
T. G. Ruksha
The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
Бюллетень сибирской медицины
melanoma of the skin
braf
nras
mutations
title The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
title_full The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
title_fullStr The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
title_full_unstemmed The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
title_short The combination of mutations in BRAF and NRAS genes within a one tumor in patients with cutaneous melanoma
title_sort combination of mutations in braf and nras genes within a one tumor in patients with cutaneous melanoma
topic melanoma of the skin
braf
nras
mutations
url https://bulletin.ssmu.ru/jour/article/view/2420
work_keys_str_mv AT mbaksenenko thecombinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma
AT asaverchuk thecombinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma
AT tgruksha thecombinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma
AT mbaksenenko combinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma
AT asaverchuk combinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma
AT tgruksha combinationofmutationsinbrafandnrasgeneswithinaonetumorinpatientswithcutaneousmelanoma