Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils
The effect of hydrocortisone on the production of interleukin-6 (IL-6) in human peripheral blood mononuclear cells was studied. Using our newly developed radioimmunoassay system for IL-6 of which specificity, reproducibility, sensitivity and usefulness have been demonstrated. IL-6 production in peri...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000036 |
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| _version_ | 1849473456899358720 |
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| author | Y. Hirooka T. Mitsuma T. Nogimori Y. Ishizukis |
| author_facet | Y. Hirooka T. Mitsuma T. Nogimori Y. Ishizukis |
| author_sort | Y. Hirooka |
| collection | DOAJ |
| description | The effect of hydrocortisone on the production of interleukin-6 (IL-6) in human peripheral blood mononuclear
cells was studied. Using our newly developed radioimmunoassay
system for IL-6 of which specificity, reproducibility,
sensitivity and usefulness have been demonstrated.
IL-6 production in peripheral blood mononuclear
cells of ten normal subjects revealed that in lipopolysaccharide
(LPS, 10μg/ml)-stimulation, the mean ± SD
of IL-6 was 2.71 ± 0.85 ng/ml. No detectable amount of
IL-6 was observed in the absence of LPS and in
the presence of hydrocortisone alone. Hydrocortisone
(10−10 M to 10−3 M) inhibited LPS-stimulated IL-6 production
in a dose-dependent manner. However, there was
a wide variation in the response to hydrocortisone, namely,
ranging from steroid-sensitive to steroid-resistant. Based
on the concentration required to inhibit 50% of LPS-stimulated
IL-6 production, three of ten subjects were at
10−6 M, three at 10−5 M and the rest at
10−4 M, respectively.
The dramatic anti-inflammatory and immunosuppressive
effects of glucocorticosteroids can be life-saving
in autoimmune diseases. The present findings suggested
that there existed the differences in susceptibility to glucocorticosteroids
even among normal subjects, providing some implications for the drug treatment, and also gave
further evidence that there may exist an immunoregulatory feedback circuit
between the immune and neuroendocrine systems. |
| format | Article |
| id | doaj-art-d2426cc133c04c52a56be40d4d67afcf |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d2426cc133c04c52a56be40d4d67afcf2025-08-20T03:24:07ZengWileyMediators of Inflammation0962-93511466-18611992-01-011191310.1155/S0962935192000036Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceilsY. Hirooka0T. Mitsuma1T. Nogimori2Y. Ishizukis3The Fourth Department of Internal Medicine, Aichi Medical University, Yazako, Nagakute-cho, Aichi-gun, Aichi-ken, 480-11, JapanThe Fourth Department of Internal Medicine, Aichi Medical University, Yazako, Nagakute-cho, Aichi-gun, Aichi-ken, 480-11, JapanDepartment of Medicine, Konanshowa Hospital, Konan, Aichi, JapanIshizuki Thyroid Clinic, Nagoya, JapanThe effect of hydrocortisone on the production of interleukin-6 (IL-6) in human peripheral blood mononuclear cells was studied. Using our newly developed radioimmunoassay system for IL-6 of which specificity, reproducibility, sensitivity and usefulness have been demonstrated. IL-6 production in peripheral blood mononuclear cells of ten normal subjects revealed that in lipopolysaccharide (LPS, 10μg/ml)-stimulation, the mean ± SD of IL-6 was 2.71 ± 0.85 ng/ml. No detectable amount of IL-6 was observed in the absence of LPS and in the presence of hydrocortisone alone. Hydrocortisone (10−10 M to 10−3 M) inhibited LPS-stimulated IL-6 production in a dose-dependent manner. However, there was a wide variation in the response to hydrocortisone, namely, ranging from steroid-sensitive to steroid-resistant. Based on the concentration required to inhibit 50% of LPS-stimulated IL-6 production, three of ten subjects were at 10−6 M, three at 10−5 M and the rest at 10−4 M, respectively. The dramatic anti-inflammatory and immunosuppressive effects of glucocorticosteroids can be life-saving in autoimmune diseases. The present findings suggested that there existed the differences in susceptibility to glucocorticosteroids even among normal subjects, providing some implications for the drug treatment, and also gave further evidence that there may exist an immunoregulatory feedback circuit between the immune and neuroendocrine systems.http://dx.doi.org/10.1155/S0962935192000036 |
| spellingShingle | Y. Hirooka T. Mitsuma T. Nogimori Y. Ishizukis Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils Mediators of Inflammation |
| title | Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils |
| title_full | Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils |
| title_fullStr | Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils |
| title_full_unstemmed | Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils |
| title_short | Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils |
| title_sort | effect of hydrocortisone on interleukin 6 production in human peripheral blood rnononuclear ceils |
| url | http://dx.doi.org/10.1155/S0962935192000036 |
| work_keys_str_mv | AT yhirooka effectofhydrocortisoneoninterleukin6productioninhumanperipheralbloodrnononuclearceils AT tmitsuma effectofhydrocortisoneoninterleukin6productioninhumanperipheralbloodrnononuclearceils AT tnogimori effectofhydrocortisoneoninterleukin6productioninhumanperipheralbloodrnononuclearceils AT yishizukis effectofhydrocortisoneoninterleukin6productioninhumanperipheralbloodrnononuclearceils |