Effect of hydrocortisone on interleukin-6 production in human ,peripheral blood rnononuclear ceils
The effect of hydrocortisone on the production of interleukin-6 (IL-6) in human peripheral blood mononuclear cells was studied. Using our newly developed radioimmunoassay system for IL-6 of which specificity, reproducibility, sensitivity and usefulness have been demonstrated. IL-6 production in peri...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000036 |
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| Summary: | The effect of hydrocortisone on the production of interleukin-6 (IL-6) in human peripheral blood mononuclear
cells was studied. Using our newly developed radioimmunoassay
system for IL-6 of which specificity, reproducibility,
sensitivity and usefulness have been demonstrated.
IL-6 production in peripheral blood mononuclear
cells of ten normal subjects revealed that in lipopolysaccharide
(LPS, 10μg/ml)-stimulation, the mean ± SD
of IL-6 was 2.71 ± 0.85 ng/ml. No detectable amount of
IL-6 was observed in the absence of LPS and in
the presence of hydrocortisone alone. Hydrocortisone
(10−10 M to 10−3 M) inhibited LPS-stimulated IL-6 production
in a dose-dependent manner. However, there was
a wide variation in the response to hydrocortisone, namely,
ranging from steroid-sensitive to steroid-resistant. Based
on the concentration required to inhibit 50% of LPS-stimulated
IL-6 production, three of ten subjects were at
10−6 M, three at 10−5 M and the rest at
10−4 M, respectively.
The dramatic anti-inflammatory and immunosuppressive
effects of glucocorticosteroids can be life-saving
in autoimmune diseases. The present findings suggested
that there existed the differences in susceptibility to glucocorticosteroids
even among normal subjects, providing some implications for the drug treatment, and also gave
further evidence that there may exist an immunoregulatory feedback circuit
between the immune and neuroendocrine systems. |
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| ISSN: | 0962-9351 1466-1861 |