Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis
Pentalenolactone is a sesquiterpene antibiotic from Streptomyces. Its biosynthetic pathway has been elucidated, except for the oxidation of pentalen-13-al to 1-deoxypentalenic acid. In this study, we show that cytochrome P450 pentalenene oxygenase catalyzed the formation of 1-deoxypentalenic acid. F...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Engineering Microbiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667370325000207 |
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| author | Jing Li Chengde Zhang Shiwen Wu Jiao Xue Ke Chen Zixin Deng Dongqing Zhu |
| author_facet | Jing Li Chengde Zhang Shiwen Wu Jiao Xue Ke Chen Zixin Deng Dongqing Zhu |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Pentalenolactone is a sesquiterpene antibiotic from Streptomyces. Its biosynthetic pathway has been elucidated, except for the oxidation of pentalen-13-al to 1-deoxypentalenic acid. In this study, we show that cytochrome P450 pentalenene oxygenase catalyzed the formation of 1-deoxypentalenic acid. Ferredoxin XNR_5179 and ferredoxin reductase XNR_4478 from S. albus are suitable redox proteins for pentalenene oxygenase. The biosynthetic pathway presented fills a gap in the biosynthetic pathway of pentalenolactone and provides an example of cytochrome P450 enzyme activity being affected by redox proteins. |
| format | Article |
| id | doaj-art-d2422ef1dc7b489ebbefd61041767437 |
| institution | Kabale University |
| issn | 2667-3703 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Engineering Microbiology |
| spelling | doaj-art-d2422ef1dc7b489ebbefd610417674372025-08-20T03:30:31ZengElsevierEngineering Microbiology2667-37032025-06-015210020610.1016/j.engmic.2025.100206Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesisJing Li0Chengde Zhang1Shiwen Wu2Jiao Xue3Ke Chen4Zixin Deng5Dongqing Zhu6The Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, China; Biomedicine Research Center, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, ChinaThe Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan 430071, China; Corresponding author.Pentalenolactone is a sesquiterpene antibiotic from Streptomyces. Its biosynthetic pathway has been elucidated, except for the oxidation of pentalen-13-al to 1-deoxypentalenic acid. In this study, we show that cytochrome P450 pentalenene oxygenase catalyzed the formation of 1-deoxypentalenic acid. Ferredoxin XNR_5179 and ferredoxin reductase XNR_4478 from S. albus are suitable redox proteins for pentalenene oxygenase. The biosynthetic pathway presented fills a gap in the biosynthetic pathway of pentalenolactone and provides an example of cytochrome P450 enzyme activity being affected by redox proteins.http://www.sciencedirect.com/science/article/pii/S2667370325000207StreptomycesPentalenene oxygenaseFerredoxinFerredoxin reductasePentalenolactone |
| spellingShingle | Jing Li Chengde Zhang Shiwen Wu Jiao Xue Ke Chen Zixin Deng Dongqing Zhu Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis Engineering Microbiology Streptomyces Pentalenene oxygenase Ferredoxin Ferredoxin reductase Pentalenolactone |
| title | Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis |
| title_full | Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis |
| title_fullStr | Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis |
| title_full_unstemmed | Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis |
| title_short | Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis |
| title_sort | cytochrome p450 catalyzed allylic oxidation of pentalenene to 1 deoxypentalenic acid in pentalenolactone biosynthesis |
| topic | Streptomyces Pentalenene oxygenase Ferredoxin Ferredoxin reductase Pentalenolactone |
| url | http://www.sciencedirect.com/science/article/pii/S2667370325000207 |
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