Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i>
Despite the existing effective treatment methods, tuberculosis (TB) is the second most deadly infectious disease, its carriers in the latent and active phases accounting for more than 20% of the world population. An effective method for controlling TB and reducing TB mortality is regular population...
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MDPI AG
2024-09-01
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| author | Viktor V. Nikolaev Tatiana B. Lepekhina Alexander S. Alliluev Elham Bidram Pavel M. Sokolov Igor R. Nabiev Yury V. Kistenev |
| author_facet | Viktor V. Nikolaev Tatiana B. Lepekhina Alexander S. Alliluev Elham Bidram Pavel M. Sokolov Igor R. Nabiev Yury V. Kistenev |
| author_sort | Viktor V. Nikolaev |
| collection | DOAJ |
| description | Despite the existing effective treatment methods, tuberculosis (TB) is the second most deadly infectious disease, its carriers in the latent and active phases accounting for more than 20% of the world population. An effective method for controlling TB and reducing TB mortality is regular population screening aimed at diagnosing the latent form of TB and taking preventive and curative measures. Numerous methods allow diagnosing TB by directly detecting <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>) biomarkers, including <i>M.tb</i> DNA, proteins, and specific metabolites or antibodies produced by the host immune system in response to <i>M.tb.</i> PCR, ELISA, immunofluorescence and immunochemical analyses, flow cytometry, and other methods allow the detection of <i>M.tb</i> biomarkers or the host immune response to <i>M.tb</i> by recording the optical signal from fluorescent or colorimetric dyes that are components of the diagnostic systems. Current research in biosensors is aimed at increasing the sensitivity of detection, a promising approach being the use of fluorescent quantum dots as brighter and more photostable optical tags. Here, we review current methods for the detection of <i>M.tb</i> biomarkers using quantum dot-based nanosensors and summarize data on the <i>M.tb</i> biomarkers whose detection can be made considerably more sensitive by using these sensors. |
| format | Article |
| id | doaj-art-d2421cc91c444c0a9d8d8546ee109095 |
| institution | OA Journals |
| issn | 2079-4991 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-d2421cc91c444c0a9d8d8546ee1090952025-08-20T02:16:55ZengMDPI AGNanomaterials2079-49912024-09-011419155310.3390/nano14191553Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i>Viktor V. Nikolaev0Tatiana B. Lepekhina1Alexander S. Alliluev2Elham Bidram3Pavel M. Sokolov4Igor R. Nabiev5Yury V. Kistenev6Laboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, 634050 Tomsk, RussiaLaboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, 634050 Tomsk, RussiaLaboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, 634050 Tomsk, RussiaDepartment of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, IranLife Improvement by Future Technologies (LIFT) Center, Skolkovo, 143025 Moscow, RussiaLife Improvement by Future Technologies (LIFT) Center, Skolkovo, 143025 Moscow, RussiaLaboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, 634050 Tomsk, RussiaDespite the existing effective treatment methods, tuberculosis (TB) is the second most deadly infectious disease, its carriers in the latent and active phases accounting for more than 20% of the world population. An effective method for controlling TB and reducing TB mortality is regular population screening aimed at diagnosing the latent form of TB and taking preventive and curative measures. Numerous methods allow diagnosing TB by directly detecting <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>) biomarkers, including <i>M.tb</i> DNA, proteins, and specific metabolites or antibodies produced by the host immune system in response to <i>M.tb.</i> PCR, ELISA, immunofluorescence and immunochemical analyses, flow cytometry, and other methods allow the detection of <i>M.tb</i> biomarkers or the host immune response to <i>M.tb</i> by recording the optical signal from fluorescent or colorimetric dyes that are components of the diagnostic systems. Current research in biosensors is aimed at increasing the sensitivity of detection, a promising approach being the use of fluorescent quantum dots as brighter and more photostable optical tags. Here, we review current methods for the detection of <i>M.tb</i> biomarkers using quantum dot-based nanosensors and summarize data on the <i>M.tb</i> biomarkers whose detection can be made considerably more sensitive by using these sensors.https://www.mdpi.com/2079-4991/14/19/1553quantum dotnanosensortuberculosisdiagnostics |
| spellingShingle | Viktor V. Nikolaev Tatiana B. Lepekhina Alexander S. Alliluev Elham Bidram Pavel M. Sokolov Igor R. Nabiev Yury V. Kistenev Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> Nanomaterials quantum dot nanosensor tuberculosis diagnostics |
| title | Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> |
| title_full | Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> |
| title_fullStr | Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> |
| title_full_unstemmed | Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> |
| title_short | Quantum Dot-Based Nanosensors for In Vitro Detection of <i>Mycobacterium tuberculosis</i> |
| title_sort | quantum dot based nanosensors for in vitro detection of i mycobacterium tuberculosis i |
| topic | quantum dot nanosensor tuberculosis diagnostics |
| url | https://www.mdpi.com/2079-4991/14/19/1553 |
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