BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU
Aim: to evaluate the biomechanical remodeling of polymer grafts modified with vascular endothelial growth factor (VEGF) after implantation into rat abdominal aorta.Materials and methods. Vascular grafts of2 mmdiameter were fabricated by electrospinning from polycaprolactone (PCL) and a mixture of po...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2016-06-01
|
| Series: | Вестник трансплантологии и искусственных органов |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/644 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849338564283727872 |
|---|---|
| author | T. V. Glushkova V. V. Sevostyanova L. V. Antonova K. Yu. Klyshnikov E. A. Ovcharenko E. A. Sergeeva G. Yu. Vasyukov A. M. Seifalian L. S. Barbarash |
| author_facet | T. V. Glushkova V. V. Sevostyanova L. V. Antonova K. Yu. Klyshnikov E. A. Ovcharenko E. A. Sergeeva G. Yu. Vasyukov A. M. Seifalian L. S. Barbarash |
| author_sort | T. V. Glushkova |
| collection | DOAJ |
| description | Aim: to evaluate the biomechanical remodeling of polymer grafts modified with vascular endothelial growth factor (VEGF) after implantation into rat abdominal aorta.Materials and methods. Vascular grafts of2 mmdiameter were fabricated by electrospinning from polycaprolactone (PCL) and a mixture of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) and PCL. The grafts were modified with VEGF by biphasic electrospinning. Morphology of the grafts was assessed by scanning electron microscopy. Physico-mechanical properties of PCL and PHBV/PCL grafts were estimated using uniaxial tensile test and physiological circulating system equipped with state-of-theart ultrasound vascular wall tracking system. Physico-mechanical testing of PCL/VEGF and PHBV/PCL/VEGF was performed before and after implantation into rat abdominal aorta for 6 months. The modeling of coronary artery bypass grafting (CABG) was performed by finite element analysis for modified grafts.Results. Durability of PCL and PHBV/PCL grafts did not differ from that of human internal mammary artery; however, elasticity and stiffness of these grafts were higher compared to internal mammary artery. Viscoelastic properties of the grafts were comparable to those of native blood vessels. Modification of the grafts with VEGF reduced material stiffness. Six months postimplantation, PCL/VEGF and PHBV/PCL/VEGF were integrated with aortic tissue that induced changes in the physico-mechanical properties of the grafts similar to the native vessel. Biomechanical modeling confirmed the functioning of modified grafts in bypass position for CABG.Conclusion. PCL/VEGF and PHBV/PCL/VEGF grafts have satisfactory physico-mechanical properties and can be potentially used in the reconstruction of blood vessels. |
| format | Article |
| id | doaj-art-d2408add4ccf4f428e73da2e57758674 |
| institution | Kabale University |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2016-06-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-d2408add4ccf4f428e73da2e577586742025-08-20T03:44:21ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912016-06-011829910910.15825/1995-1191-2016-2-99-109544BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITUT. V. Glushkova0V. V. Sevostyanova1L. V. Antonova2K. Yu. Klyshnikov3E. A. Ovcharenko4E. A. Sergeeva5G. Yu. Vasyukov6A. M. Seifalian7L. S. Barbarash8Research Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoCentre for Nanotechnology & Regenerative Medicine, UCL Dvision of Surgery & Interventional Science, University College London (UCL) and NanoRegMed Ltd, LondonResearch Institute for Complex Issues of Cardiovascular Diseases, KemerovoAim: to evaluate the biomechanical remodeling of polymer grafts modified with vascular endothelial growth factor (VEGF) after implantation into rat abdominal aorta.Materials and methods. Vascular grafts of2 mmdiameter were fabricated by electrospinning from polycaprolactone (PCL) and a mixture of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) and PCL. The grafts were modified with VEGF by biphasic electrospinning. Morphology of the grafts was assessed by scanning electron microscopy. Physico-mechanical properties of PCL and PHBV/PCL grafts were estimated using uniaxial tensile test and physiological circulating system equipped with state-of-theart ultrasound vascular wall tracking system. Physico-mechanical testing of PCL/VEGF and PHBV/PCL/VEGF was performed before and after implantation into rat abdominal aorta for 6 months. The modeling of coronary artery bypass grafting (CABG) was performed by finite element analysis for modified grafts.Results. Durability of PCL and PHBV/PCL grafts did not differ from that of human internal mammary artery; however, elasticity and stiffness of these grafts were higher compared to internal mammary artery. Viscoelastic properties of the grafts were comparable to those of native blood vessels. Modification of the grafts with VEGF reduced material stiffness. Six months postimplantation, PCL/VEGF and PHBV/PCL/VEGF were integrated with aortic tissue that induced changes in the physico-mechanical properties of the grafts similar to the native vessel. Biomechanical modeling confirmed the functioning of modified grafts in bypass position for CABG.Conclusion. PCL/VEGF and PHBV/PCL/VEGF grafts have satisfactory physico-mechanical properties and can be potentially used in the reconstruction of blood vessels.https://journal.transpl.ru/vtio/article/view/644polycaprolactone, polyhydroxybutyrate/valerate, vascular endothelial growth factor, vascular graft, electrospinning |
| spellingShingle | T. V. Glushkova V. V. Sevostyanova L. V. Antonova K. Yu. Klyshnikov E. A. Ovcharenko E. A. Sergeeva G. Yu. Vasyukov A. M. Seifalian L. S. Barbarash BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU Вестник трансплантологии и искусственных органов polycaprolactone, polyhydroxybutyrate/valerate, vascular endothelial growth factor, vascular graft, electrospinning |
| title | BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU |
| title_full | BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU |
| title_fullStr | BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU |
| title_full_unstemmed | BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU |
| title_short | BIOMECHANICAL REMODELING OF BIODEGRADABLE SMALL-DIAMETER VASCULAR GRAFTS IN SITU |
| title_sort | biomechanical remodeling of biodegradable small diameter vascular grafts in situ |
| topic | polycaprolactone, polyhydroxybutyrate/valerate, vascular endothelial growth factor, vascular graft, electrospinning |
| url | https://journal.transpl.ru/vtio/article/view/644 |
| work_keys_str_mv | AT tvglushkova biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT vvsevostyanova biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT lvantonova biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT kyuklyshnikov biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT eaovcharenko biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT easergeeva biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT gyuvasyukov biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT amseifalian biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu AT lsbarbarash biomechanicalremodelingofbiodegradablesmalldiametervasculargraftsinsitu |