Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds
Chronic wounds complicated by infection pose significant clinical challenges, necessitating comprehensive treatment approaches. The widespread use of antibiotics has led to resistant microorganisms, complicating traditional therapies. This study aims to develop and evaluate modified alginate wound d...
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MDPI AG
2024-09-01
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| Online Access: | https://www.mdpi.com/2227-9059/12/9/2122 |
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| author | Aliya Atepileva Vyacheslav Ogay Gulshahar Kudaibergen Guldarigash Kaukabaeva Assiya Nurkina Ainur Mukhambetova Serik Balgazarov Arman Batpen Dina Saginova Zhanatay Ramazanov Amanzhol Balgazarov Zhanar Akhmetkarimova |
| author_facet | Aliya Atepileva Vyacheslav Ogay Gulshahar Kudaibergen Guldarigash Kaukabaeva Assiya Nurkina Ainur Mukhambetova Serik Balgazarov Arman Batpen Dina Saginova Zhanatay Ramazanov Amanzhol Balgazarov Zhanar Akhmetkarimova |
| author_sort | Aliya Atepileva |
| collection | DOAJ |
| description | Chronic wounds complicated by infection pose significant clinical challenges, necessitating comprehensive treatment approaches. The widespread use of antibiotics has led to resistant microorganisms, complicating traditional therapies. This study aims to develop and evaluate modified alginate wound dressings with enhanced antimicrobial and regenerative properties. Alginate dressings were synthesized with silver nanoparticles, cefepime, and fibroblast growth factor-2 (FGF-2). The two-stage therapy involved an initial antibacterial dressing followed by a regenerative dressing. In vitro tests demonstrated high antibacterial activity, with maximum inhibition zones for <i>P. aeruginosa</i> (41.3 ± 0.4 mm) and <i>S. aureus</i> (36.6 ± 1.8 mm). In vivo studies on rats with purulent wounds showed significant healing progression in the experimental group. Histological analysis revealed complete re-epithelialization, thicker neoepithelium, dense collagen deposition, and minimal inflammation in treated wounds. These findings suggest that the modified alginate dressings significantly enhance the reparative process and are promising for treating chronic infected wounds in both veterinary and medical practices. |
| format | Article |
| id | doaj-art-d23723c9e6d443c5a539fca381c28df6 |
| institution | OA Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-d23723c9e6d443c5a539fca381c28df62025-08-20T01:56:00ZengMDPI AGBiomedicines2227-90592024-09-01129212210.3390/biomedicines12092122Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent WoundsAliya Atepileva0Vyacheslav Ogay1Gulshahar Kudaibergen2Guldarigash Kaukabaeva3Assiya Nurkina4Ainur Mukhambetova5Serik Balgazarov6Arman Batpen7Dina Saginova8Zhanatay Ramazanov9Amanzhol Balgazarov10Zhanar Akhmetkarimova11Department of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanStem Cell Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanDepartment of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanDepartment of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanDepartment of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanDepartment of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanDepartment of Traumatology No. 4, National Scientific Center of Traumatology and Orthopedics Named after Academician N.D. Batpenov, Astana 010000, KazakhstanToxicology and Pharmacology Laboratory, National Center for Biotechnology, Astana 010000, KazakhstanChronic wounds complicated by infection pose significant clinical challenges, necessitating comprehensive treatment approaches. The widespread use of antibiotics has led to resistant microorganisms, complicating traditional therapies. This study aims to develop and evaluate modified alginate wound dressings with enhanced antimicrobial and regenerative properties. Alginate dressings were synthesized with silver nanoparticles, cefepime, and fibroblast growth factor-2 (FGF-2). The two-stage therapy involved an initial antibacterial dressing followed by a regenerative dressing. In vitro tests demonstrated high antibacterial activity, with maximum inhibition zones for <i>P. aeruginosa</i> (41.3 ± 0.4 mm) and <i>S. aureus</i> (36.6 ± 1.8 mm). In vivo studies on rats with purulent wounds showed significant healing progression in the experimental group. Histological analysis revealed complete re-epithelialization, thicker neoepithelium, dense collagen deposition, and minimal inflammation in treated wounds. These findings suggest that the modified alginate dressings significantly enhance the reparative process and are promising for treating chronic infected wounds in both veterinary and medical practices.https://www.mdpi.com/2227-9059/12/9/2122alginate wound dressingssilver nanoparticlescefepimeFGF-2chronic woundsantibacterial properties |
| spellingShingle | Aliya Atepileva Vyacheslav Ogay Gulshahar Kudaibergen Guldarigash Kaukabaeva Assiya Nurkina Ainur Mukhambetova Serik Balgazarov Arman Batpen Dina Saginova Zhanatay Ramazanov Amanzhol Balgazarov Zhanar Akhmetkarimova Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds Biomedicines alginate wound dressings silver nanoparticles cefepime FGF-2 chronic wounds antibacterial properties |
| title | Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds |
| title_full | Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds |
| title_fullStr | Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds |
| title_full_unstemmed | Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds |
| title_short | Exploring the Antibacterial and Regenerative Properties of a Two-Stage Alginate Wound Dressing in a Rat Model of Purulent Wounds |
| title_sort | exploring the antibacterial and regenerative properties of a two stage alginate wound dressing in a rat model of purulent wounds |
| topic | alginate wound dressings silver nanoparticles cefepime FGF-2 chronic wounds antibacterial properties |
| url | https://www.mdpi.com/2227-9059/12/9/2122 |
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