Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1
Natural microbial pigments are gaining attention for their potential in various applications due to their safety and sustainability. In this study, we isolated a high-yielding pigment-producing fungus identified as Talaromyces atroroseus LWT-1. The predominant pigment compounds were isolated from th...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612109/full |
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| author | Xian Xia Xian Xia Li-Yu Liu Miao Liu Guo-Jun Hu Wen-Ting Li Zi-Yi Wang Yao Pei Yan-He Li Jing-Jing Li Yan-Xiang Wang Xiao-Shan Shi Jun-Ming Tu |
| author_facet | Xian Xia Xian Xia Li-Yu Liu Miao Liu Guo-Jun Hu Wen-Ting Li Zi-Yi Wang Yao Pei Yan-He Li Jing-Jing Li Yan-Xiang Wang Xiao-Shan Shi Jun-Ming Tu |
| author_sort | Xian Xia |
| collection | DOAJ |
| description | Natural microbial pigments are gaining attention for their potential in various applications due to their safety and sustainability. In this study, we isolated a high-yielding pigment-producing fungus identified as Talaromyces atroroseus LWT-1. The predominant pigment compounds were isolated from the culture extract of T. atroroseus LWT-1 through various chromatographic methods and identified as talaroconvolutins A (1) and B (2), and talarofuranone (3). Compounds 1 and 3 exhibited cytotoxic activity against MCF7, Huh-7, and H446 lines with IC50 values ranging from 0.68 ± 0.09 to 4.19 ± 0.71 μM. In contrast, compound 1 was non-toxic to BEAS-2B cells and significantly promoted their proliferation. To optimize pigments yield, we conducted a series of single-factor and orthogonal experiments to determine the optimal fermentation conditions. The optimal conditions were determined to be: SDA culture medium, 32°C incubation temperature, 170 rpm shaking speed, 60 mL working volume in a 250 mL flask, and a culture duration of 120 h. |
| format | Article |
| id | doaj-art-d22fcd96426a4cbe86896cea67313d09 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-d22fcd96426a4cbe86896cea67313d092025-08-20T02:07:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.16121091612109Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1Xian Xia0Xian Xia1Li-Yu Liu2Miao Liu3Guo-Jun Hu4Wen-Ting Li5Zi-Yi Wang6Yao Pei7Yan-He Li8Jing-Jing Li9Yan-Xiang Wang10Xiao-Shan Shi11Jun-Ming Tu12Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaLaboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, Kannapolis, NC, United StatesHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Environmental Protection and Sustainable Utilization of Resources, Hubei Engineering Research Center of Characteristic Wild Vegetable Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, ChinaNatural microbial pigments are gaining attention for their potential in various applications due to their safety and sustainability. In this study, we isolated a high-yielding pigment-producing fungus identified as Talaromyces atroroseus LWT-1. The predominant pigment compounds were isolated from the culture extract of T. atroroseus LWT-1 through various chromatographic methods and identified as talaroconvolutins A (1) and B (2), and talarofuranone (3). Compounds 1 and 3 exhibited cytotoxic activity against MCF7, Huh-7, and H446 lines with IC50 values ranging from 0.68 ± 0.09 to 4.19 ± 0.71 μM. In contrast, compound 1 was non-toxic to BEAS-2B cells and significantly promoted their proliferation. To optimize pigments yield, we conducted a series of single-factor and orthogonal experiments to determine the optimal fermentation conditions. The optimal conditions were determined to be: SDA culture medium, 32°C incubation temperature, 170 rpm shaking speed, 60 mL working volume in a 250 mL flask, and a culture duration of 120 h.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612109/fullnatural pigmentsTalaromyces atroroseuspurificationcytotoxicity assayproduction optimization |
| spellingShingle | Xian Xia Xian Xia Li-Yu Liu Miao Liu Guo-Jun Hu Wen-Ting Li Zi-Yi Wang Yao Pei Yan-He Li Jing-Jing Li Yan-Xiang Wang Xiao-Shan Shi Jun-Ming Tu Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 Frontiers in Microbiology natural pigments Talaromyces atroroseus purification cytotoxicity assay production optimization |
| title | Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 |
| title_full | Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 |
| title_fullStr | Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 |
| title_full_unstemmed | Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 |
| title_short | Isolation, identification, and production optimization of natural functional pigments produced by Talaromyces atroroseus LWT-1 |
| title_sort | isolation identification and production optimization of natural functional pigments produced by talaromyces atroroseus lwt 1 |
| topic | natural pigments Talaromyces atroroseus purification cytotoxicity assay production optimization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612109/full |
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