Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选)
Paenonia lactiflora Pall. is a renowned traditional Chinese flower, and it is of great significance to study its cold tolerance mechanism for breeding new varieties and expanding its popularization. In this study, we compared ‘Hang Baishao’, a low-altitude southern variety with concentrated industri...
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Zhejiang University Press
2025-02-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://doi.org/10.3785/j.issn.1008-9209.2024.12.091 |
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| author | 王琪瑶(WANG Qiyao) 李丹青(LI Danqing) 郭俊宏(GUO Junhong) 陈晓璇(CHEN Xiaoxuan) 陈炀(CHEN Yang) 夏宜平(XIA Yiping) 张佳平(ZHANG Jiaping) 刘志洋(LIU Zhiyang) |
| author_facet | 王琪瑶(WANG Qiyao) 李丹青(LI Danqing) 郭俊宏(GUO Junhong) 陈晓璇(CHEN Xiaoxuan) 陈炀(CHEN Yang) 夏宜平(XIA Yiping) 张佳平(ZHANG Jiaping) 刘志洋(LIU Zhiyang) |
| author_sort | 王琪瑶(WANG Qiyao) |
| collection | DOAJ |
| description | Paenonia lactiflora Pall. is a renowned traditional Chinese flower, and it is of great significance to study its cold tolerance mechanism for breeding new varieties and expanding its popularization. In this study, we compared ‘Hang Baishao’, a low-altitude southern variety with concentrated industrial cultivation, with ‘Hongling Chijin’, a northern variety with superior performance in Harbin, both of which are grown in open fields of Harbin during the winter. We investigated the physiological and molecular responses of their rhizomes to cold stress under freezing conditions through physiological observations, transcriptome sequencing, and gene expression analysis. The results showed that as winter temperature decreased, the levels of soluble sugars, abscisic acid, auxins, and cytokinins in ‘Hongling Chijin’ increased more markedly than those in ‘Hang Baishao’, while gibberellin levels decreased. The omics analysis showed that genes related to starch and sucrose metabolism, plant-pathogen interaction, and hormone signaling were significantly differentially expressed between two varieties. Co-expression analysis showed that, genes such as dehydration-responsive element-binding protein 2A gene (DREB2A),cysteine-rich receptor-like kinase 8 gene (CRK8),and heat shock protein 90.1 gene (HSP90.1) may regulate herbaceous peony’s cold stress response. This study can preliminarily reveal the key genes of herbaceous peony’s response to cold stress in extremely cold weather, lay a foundation for the subsequent study of gene function and gene interaction, and contribute to the cultivation of new varieties of herbaceous peony that can be widely cultivated and bred at China’s highest latitudes.
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芍药（Paenonia lactiflora Pall.）是我国传统名花，研究其耐寒机制对培育新品种、扩大推广范围具有重要意义。本研究采用我国集中产业化生产的最南方且低海拔的芍药‘杭白芍’和在哈尔滨引种表现最优的北方品种‘红绫赤金’作为实验材料，栽培于哈尔滨露地条件下过冬，通过生理观测、转录组测序和基因定量表达分析，研究芍药根茎在极寒天气冻土层之下的冷胁迫生理和分子响应情况。结果显示：随着冬季温度逐渐降低，‘红绫赤金’根茎中的可溶性糖、脱落酸、生长素、细胞分裂素含量相较于‘杭白芍’显著增加，而赤霉素含量则降低；组学分析表明，与淀粉和蔗糖代谢、植物-病原体互作、植物激素信号转导等通路相关的基因在2个品种间发生显著的差异性表达；共表达分析表明，脱水反应元件结合蛋白2A基因（dehydration-responsive element-binding protein 2A gene, DREB2A）、富含半胱氨酸类受体激酶8基因（cysteine-rich receptor-like kinase 8 gene, CRK8）、热激蛋白90.1基因（heat shock protein 90.1 gene, HSP90.1）等可能在调控芍药应对冷胁迫中发挥关键作用。本研究可初步揭示芍药应对极寒天气下的冷胁迫关键响应基因，为后续的基因功能、基因互作研究奠定基础，并为培育能够在我国最高纬度地区广泛栽培繁殖的芍药耐寒新品种作出贡献。 |
| format | Article |
| id | doaj-art-d22229d6cc5747e4a92f6f38fd25b367 |
| institution | Kabale University |
| issn | 2097-5155 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-d22229d6cc5747e4a92f6f38fd25b3672025-08-20T03:41:44ZengZhejiang University Press浙江大学学报. 农业与生命科学版2097-51552025-02-0151114816310.3785/j.issn.1008-9209.2024.12.09110089209Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选)王琪瑶(WANG Qiyao)0https://orcid.org/0009-0001-3102-1010李丹青(LI Danqing)1郭俊宏(GUO Junhong)2陈晓璇(CHEN Xiaoxuan)3陈炀(CHEN Yang)4夏宜平(XIA Yiping)5张佳平(ZHANG Jiaping)6https://orcid.org/0000-0003-1000-4542刘志洋(LIU Zhiyang)7https://orcid.org/0009-0003-1383-9147 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 2 School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China( 2 浙江理工大学建筑工程学院，浙江 杭州 310018) 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 1 Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China( 1 浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058) 3 College of Life Science, Northeast Forestry University, Harbin 150040, Heilongjiang, China( 3 东北林业大学生命科学学院，黑龙江 哈尔滨 150040)Paenonia lactiflora Pall. is a renowned traditional Chinese flower, and it is of great significance to study its cold tolerance mechanism for breeding new varieties and expanding its popularization. In this study, we compared ‘Hang Baishao’, a low-altitude southern variety with concentrated industrial cultivation, with ‘Hongling Chijin’, a northern variety with superior performance in Harbin, both of which are grown in open fields of Harbin during the winter. We investigated the physiological and molecular responses of their rhizomes to cold stress under freezing conditions through physiological observations, transcriptome sequencing, and gene expression analysis. The results showed that as winter temperature decreased, the levels of soluble sugars, abscisic acid, auxins, and cytokinins in ‘Hongling Chijin’ increased more markedly than those in ‘Hang Baishao’, while gibberellin levels decreased. The omics analysis showed that genes related to starch and sucrose metabolism, plant-pathogen interaction, and hormone signaling were significantly differentially expressed between two varieties. Co-expression analysis showed that, genes such as dehydration-responsive element-binding protein 2A gene (DREB2A),cysteine-rich receptor-like kinase 8 gene (CRK8),and heat shock protein 90.1 gene (HSP90.1) may regulate herbaceous peony’s cold stress response. This study can preliminarily reveal the key genes of herbaceous peony’s response to cold stress in extremely cold weather, lay a foundation for the subsequent study of gene function and gene interaction, and contribute to the cultivation of new varieties of herbaceous peony that can be widely cultivated and bred at China’s highest latitudes. . 芍药（Paenonia lactiflora Pall.）是我国传统名花，研究其耐寒机制对培育新品种、扩大推广范围具有重要意义。本研究采用我国集中产业化生产的最南方且低海拔的芍药‘杭白芍’和在哈尔滨引种表现最优的北方品种‘红绫赤金’作为实验材料，栽培于哈尔滨露地条件下过冬，通过生理观测、转录组测序和基因定量表达分析，研究芍药根茎在极寒天气冻土层之下的冷胁迫生理和分子响应情况。结果显示：随着冬季温度逐渐降低，‘红绫赤金’根茎中的可溶性糖、脱落酸、生长素、细胞分裂素含量相较于‘杭白芍’显著增加，而赤霉素含量则降低；组学分析表明，与淀粉和蔗糖代谢、植物-病原体互作、植物激素信号转导等通路相关的基因在2个品种间发生显著的差异性表达；共表达分析表明，脱水反应元件结合蛋白2A基因（dehydration-responsive element-binding protein 2A gene, DREB2A）、富含半胱氨酸类受体激酶8基因（cysteine-rich receptor-like kinase 8 gene, CRK8）、热激蛋白90.1基因（heat shock protein 90.1 gene, HSP90.1）等可能在调控芍药应对冷胁迫中发挥关键作用。本研究可初步揭示芍药应对极寒天气下的冷胁迫关键响应基因，为后续的基因功能、基因互作研究奠定基础，并为培育能够在我国最高纬度地区广泛栽培繁殖的芍药耐寒新品种作出贡献。https://doi.org/10.3785/j.issn.1008-9209.2024.12.091芍药(herbaceous peony)冷胁迫(cold stress)根茎(rhizome)耐寒生理(cold tolerance physiology)转录组(transcriptome)加权基因共表达网络分析(weighted gene co-expression network analysis (wgcna)) |
| spellingShingle | 王琪瑶(WANG Qiyao) 李丹青(LI Danqing) 郭俊宏(GUO Junhong) 陈晓璇(CHEN Xiaoxuan) 陈炀(CHEN Yang) 夏宜平(XIA Yiping) 张佳平(ZHANG Jiaping) 刘志洋(LIU Zhiyang) Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) 浙江大学学报. 农业与生命科学版 芍药(herbaceous peony) 冷胁迫(cold stress) 根茎(rhizome) 耐寒生理(cold tolerance physiology) 转录组(transcriptome) 加权基因共表达网络分析(weighted gene co-expression network analysis (wgcna)) |
| title | Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) |
| title_full | Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) |
| title_fullStr | Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) |
| title_full_unstemmed | Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) |
| title_short | Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选) |
| title_sort | physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in harbin 哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选 |
| topic | 芍药(herbaceous peony) 冷胁迫(cold stress) 根茎(rhizome) 耐寒生理(cold tolerance physiology) 转录组(transcriptome) 加权基因共表达网络分析(weighted gene co-expression network analysis (wgcna)) |
| url | https://doi.org/10.3785/j.issn.1008-9209.2024.12.091 |
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