Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery
Summary: The number of reliable and non-invasive techniques to characterize ocular blood flow is limited. Here, we present a protocol for identifying a soft tissue window in the orbital aperture using pulsed-Doppler ultrasonography to offer real-time, non-invasive blood flow velocity monitoring in t...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002990 |
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| author | Radwa Awad Emil Martin Anilkumar K. Reddy Iraida Sharina |
| author_facet | Radwa Awad Emil Martin Anilkumar K. Reddy Iraida Sharina |
| author_sort | Radwa Awad |
| collection | DOAJ |
| description | Summary: The number of reliable and non-invasive techniques to characterize ocular blood flow is limited. Here, we present a protocol for identifying a soft tissue window in the orbital aperture using pulsed-Doppler ultrasonography to offer real-time, non-invasive blood flow velocity monitoring in the mouse ophthalmic artery. We describe steps for preparing a mouse, positioning the ultrasound probe, and acquiring and recording signals. We then detail procedures for analyzing results using a workstation equipped with Doppler flow velocity software.For complete details on the use and execution of this protocol, please refer to Sharina et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-d22087da8caa40159b04b46fe23e252d |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-d22087da8caa40159b04b46fe23e252d2025-08-20T03:21:52ZengElsevierSTAR Protocols2666-16672025-09-016310389310.1016/j.xpro.2025.103893Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic arteryRadwa Awad0Emil Martin1Anilkumar K. Reddy2Iraida Sharina3Cardiology Division, Department of Internal Medicine, The University of Texas-McGovern Medical School, Houston, TX 77054, USACardiology Division, Department of Internal Medicine, The University of Texas-McGovern Medical School, Houston, TX 77054, USASection of Cardiovascular Research, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA; Indus Instruments, Webster, TX 77598, USA; Corresponding authorCardiology Division, Department of Internal Medicine, The University of Texas-McGovern Medical School, Houston, TX 77054, USA; Corresponding authorSummary: The number of reliable and non-invasive techniques to characterize ocular blood flow is limited. Here, we present a protocol for identifying a soft tissue window in the orbital aperture using pulsed-Doppler ultrasonography to offer real-time, non-invasive blood flow velocity monitoring in the mouse ophthalmic artery. We describe steps for preparing a mouse, positioning the ultrasound probe, and acquiring and recording signals. We then detail procedures for analyzing results using a workstation equipped with Doppler flow velocity software.For complete details on the use and execution of this protocol, please refer to Sharina et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002990Health SciencesModel OrganismsMolecular Biology |
| spellingShingle | Radwa Awad Emil Martin Anilkumar K. Reddy Iraida Sharina Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery STAR Protocols Health Sciences Model Organisms Molecular Biology |
| title | Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery |
| title_full | Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery |
| title_fullStr | Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery |
| title_full_unstemmed | Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery |
| title_short | Protocol for non-invasive pulsed-Doppler evaluation of blood flow in the mouse ophthalmic artery |
| title_sort | protocol for non invasive pulsed doppler evaluation of blood flow in the mouse ophthalmic artery |
| topic | Health Sciences Model Organisms Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002990 |
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