Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities
This study assessed the effect of simulated human digestion on the phytochemical properties and antioxidant capacity of Amazonian pataua (Oenocarpus bataua) pulp extract in human cells and plasma, and the antimalarial effect against Plasmodium falciparum. The composition of the pulp extract before a...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833525001121 |
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| author | Amanda dos Santos Lima Thiago Mendanha Cruz Alessandra Oliveira Silva Tanya Cox Júlia Vitória Barbosa Dias Jaqueline de Araújo Bezerra Marcos dos Santos Lima Marcell Crispim Luciana Azevedo Marciane Magnani Daniel Granato |
| author_facet | Amanda dos Santos Lima Thiago Mendanha Cruz Alessandra Oliveira Silva Tanya Cox Júlia Vitória Barbosa Dias Jaqueline de Araújo Bezerra Marcos dos Santos Lima Marcell Crispim Luciana Azevedo Marciane Magnani Daniel Granato |
| author_sort | Amanda dos Santos Lima |
| collection | DOAJ |
| description | This study assessed the effect of simulated human digestion on the phytochemical properties and antioxidant capacity of Amazonian pataua (Oenocarpus bataua) pulp extract in human cells and plasma, and the antimalarial effect against Plasmodium falciparum. The composition of the pulp extract before and after in vitro digestion was analysed by liquid chromatography. Eleven polyphenols were identified and quantified, where (+)-catechin, vanillic acid and isorhamnetin were the primary compounds. The initial screening of the water-ethanol extract (50:50 v/v) showed cytotoxicity against normal cells after digestion, justified by increased intracellular reactive oxygen species (ROS). When subjected to an in vitro digestion process, the extract’s oxygen radical absorbance capacity, iron-reducing capacity, and human plasma protection were adversely decreased. However, undigested and digested pataua pulp extracts could reduce intracellular reactive oxygen species generation in red blood cells. Moreover, both undigested and digested pataua pulp extracts showed antimalarial activity against Plasmodium falciparum, with the digested extract being more promising due to its higher potency and greater selectivity, as indicated by a lower IC50 and higher selectivity index. This study provides a solid foundation for rationally exploiting pataua as a novel source of biologically active compounds. |
| format | Article |
| id | doaj-art-d2140ff80c834f1fb12df2dd137c04ae |
| institution | Kabale University |
| issn | 2666-8335 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj-art-d2140ff80c834f1fb12df2dd137c04ae2025-08-20T03:52:48ZengElsevierFuture Foods2666-83352025-06-011110065010.1016/j.fufo.2025.100650Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activitiesAmanda dos Santos Lima0Thiago Mendanha Cruz1Alessandra Oliveira Silva2Tanya Cox3Júlia Vitória Barbosa Dias4Jaqueline de Araújo Bezerra5Marcos dos Santos Lima6Marcell Crispim7Luciana Azevedo8Marciane Magnani9Daniel Granato10University of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Bioactivity and Applications Laboratory, V94 T9PX Limerick, Ireland; Laboratory of Nutritional and Toxicological Analysis In vitro and in vivo, Federal University of Alfenas, Alfenas, 37130-001, BrazilUniversity of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Bioactivity and Applications Laboratory, V94 T9PX Limerick, IrelandUniversity of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Bioactivity and Applications Laboratory, V94 T9PX Limerick, IrelandUniversity of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Bioactivity and Applications Laboratory, V94 T9PX Limerick, IrelandFederal University of Paraíba, Department of Food Engineering, Laboratory of Microbial Processes in Foods, Campus I - University City, João Pessoa, 58051-900, BrazilAnalytical Center, Campus Manaus Center, Federal Institute of Education, Science and Technology of Amazonas, Manaus, 69020-120, BrazilDepartment of Food Technology, Federal Institute of Sertão de Pernambuco, Petrolina, BrazilDepartment of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, 37130-001, Brazil; Laboratory of Nutritional and Toxicological Analysis In vitro and in vivo, Federal University of Alfenas, Alfenas, 37130-001, BrazilLaboratory of Nutritional and Toxicological Analysis In vitro and in vivo, Federal University of Alfenas, Alfenas, 37130-001, BrazilFederal University of Paraíba, Department of Food Engineering, Laboratory of Microbial Processes in Foods, Campus I - University City, João Pessoa, 58051-900, BrazilUniversity of Limerick, Faculty of Science and Engineering, Department of Biological Sciences, Bioactivity and Applications Laboratory, V94 T9PX Limerick, Ireland; Corresponding author.This study assessed the effect of simulated human digestion on the phytochemical properties and antioxidant capacity of Amazonian pataua (Oenocarpus bataua) pulp extract in human cells and plasma, and the antimalarial effect against Plasmodium falciparum. The composition of the pulp extract before and after in vitro digestion was analysed by liquid chromatography. Eleven polyphenols were identified and quantified, where (+)-catechin, vanillic acid and isorhamnetin were the primary compounds. The initial screening of the water-ethanol extract (50:50 v/v) showed cytotoxicity against normal cells after digestion, justified by increased intracellular reactive oxygen species (ROS). When subjected to an in vitro digestion process, the extract’s oxygen radical absorbance capacity, iron-reducing capacity, and human plasma protection were adversely decreased. However, undigested and digested pataua pulp extracts could reduce intracellular reactive oxygen species generation in red blood cells. Moreover, both undigested and digested pataua pulp extracts showed antimalarial activity against Plasmodium falciparum, with the digested extract being more promising due to its higher potency and greater selectivity, as indicated by a lower IC50 and higher selectivity index. This study provides a solid foundation for rationally exploiting pataua as a novel source of biologically active compounds.http://www.sciencedirect.com/science/article/pii/S2666833525001121Plasmodium falciparumFree radicalsPolyphenolsExotic fruitsCytotoxicity |
| spellingShingle | Amanda dos Santos Lima Thiago Mendanha Cruz Alessandra Oliveira Silva Tanya Cox Júlia Vitória Barbosa Dias Jaqueline de Araújo Bezerra Marcos dos Santos Lima Marcell Crispim Luciana Azevedo Marciane Magnani Daniel Granato Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities Future Foods Plasmodium falciparum Free radicals Polyphenols Exotic fruits Cytotoxicity |
| title | Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| title_full | Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| title_fullStr | Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| title_full_unstemmed | Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| title_short | Amazonian pataua (Oenocarpus bataua) pulp extract: bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| title_sort | amazonian pataua oenocarpus bataua pulp extract bioaccessibility of phenolic compounds and effects on cellular antioxidant and antimalarial activities |
| topic | Plasmodium falciparum Free radicals Polyphenols Exotic fruits Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2666833525001121 |
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