Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia
Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HH Publisher
2021-09-01
|
| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/503 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832541440115212288 |
|---|---|
| author | Vurmila Venggadasamy Loh Teng-Hern Tan Jodi Woan-Fei Law Hooi-Leng Ser Vengadesh Letchumanan Priyia Pusparajah |
| author_facet | Vurmila Venggadasamy Loh Teng-Hern Tan Jodi Woan-Fei Law Hooi-Leng Ser Vengadesh Letchumanan Priyia Pusparajah |
| author_sort | Vurmila Venggadasamy |
| collection | DOAJ |
| description |
Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently.
|
| format | Article |
| id | doaj-art-d213f618a3bd438ab480188ef969fdfd |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-d213f618a3bd438ab480188ef969fdfd2025-02-04T08:40:13ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492021-09-014110.36877/pmmb.a0000233Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, MalaysiaVurmila Venggadasamy0Loh Teng-Hern TanJodi Woan-Fei Law1Hooi-Leng Ser2Vengadesh Letchumanan3Priyia Pusparajah4Monash UniversityMonash UniversityMonash UniversityMonash UniversityMonash University Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently. https://journals.hh-publisher.com/index.php/pmmb/article/view/503 |
| spellingShingle | Vurmila Venggadasamy Loh Teng-Hern Tan Jodi Woan-Fei Law Hooi-Leng Ser Vengadesh Letchumanan Priyia Pusparajah Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia Progress in Microbes and Molecular Biology |
| title | Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia |
| title_full | Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia |
| title_fullStr | Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia |
| title_full_unstemmed | Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia |
| title_short | Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia |
| title_sort | incidence antibiotic susceptibility and characterization of vibrio parahaemolyticus isolated from seafood in selangor malaysia |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/503 |
| work_keys_str_mv | AT vurmilavenggadasamy incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia AT lohtengherntan incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia AT jodiwoanfeilaw incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia AT hooilengser incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia AT vengadeshletchumanan incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia AT priyiapusparajah incidenceantibioticsusceptibilityandcharacterizationofvibrioparahaemolyticusisolatedfromseafoodinselangormalaysia |