Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography
Abstract A μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To op...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-025-01454-w |
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| author | Joanna Antos Laura García-Cansino María Ángeles García Dobrochna Ginter-Kramarczyk María Luisa Marina Joanna Zembrzuska José S. Câmara Jorge A. M. Pereira |
| author_facet | Joanna Antos Laura García-Cansino María Ángeles García Dobrochna Ginter-Kramarczyk María Luisa Marina Joanna Zembrzuska José S. Câmara Jorge A. M. Pereira |
| author_sort | Joanna Antos |
| collection | DOAJ |
| description | Abstract A μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency. The method was validated with optimised fast chromatographic separation, showing good linearity (R2 > 0.99), precision (RSD < 20%), and recoveries between 46-86%. Detection and quantification limits ranged from 0.30-1.23 μg L−1 and 0.92-3.73 μg L−1, respectively. The optimised μSPEed/UPLC-PDA efficiently analysed environmental water samples, requiring only 6 min extraction, 6 min analysis, and 500 μL sample, surpassing alternative methods in speed, workloads and reproducibility. The cost-effective, commercially available equipment facilitates accessibility for laboratories and adaptability for analysing selected antibiotics in diverse matrices, including food and environmental samples. |
| format | Article |
| id | doaj-art-d213d533d9e94447bbac353483f8fa21 |
| institution | DOAJ |
| issn | 2399-3669 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj-art-d213d533d9e94447bbac353483f8fa212025-08-20T03:05:56ZengNature PortfolioCommunications Chemistry2399-36692025-03-01811810.1038/s42004-025-01454-wImproved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatographyJoanna Antos0Laura García-Cansino1María Ángeles García2Dobrochna Ginter-Kramarczyk3María Luisa Marina4Joanna Zembrzuska5José S. Câmara6Jorge A. M. Pereira7CQM—Centro de Química da Madeira, Universidade da Madeira, Campus da PenteadaCQM—Centro de Química da Madeira, Universidade da Madeira, Campus da PenteadaUniversidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona Km. 33.600Department of Water Supply and Bioeconomy, Faculty of Environmental Engineering and Energy, Poznan University of Technology, Berdychowo 4Universidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid-Barcelona Km. 33.600Faculty of Chemical Technology Institute of Chemistry and Technical Electrochemistry, Berdychowo 4CQM—Centro de Química da Madeira, Universidade da Madeira, Campus da PenteadaCQM—Centro de Química da Madeira, Universidade da Madeira, Campus da PenteadaAbstract A μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency. The method was validated with optimised fast chromatographic separation, showing good linearity (R2 > 0.99), precision (RSD < 20%), and recoveries between 46-86%. Detection and quantification limits ranged from 0.30-1.23 μg L−1 and 0.92-3.73 μg L−1, respectively. The optimised μSPEed/UPLC-PDA efficiently analysed environmental water samples, requiring only 6 min extraction, 6 min analysis, and 500 μL sample, surpassing alternative methods in speed, workloads and reproducibility. The cost-effective, commercially available equipment facilitates accessibility for laboratories and adaptability for analysing selected antibiotics in diverse matrices, including food and environmental samples.https://doi.org/10.1038/s42004-025-01454-w |
| spellingShingle | Joanna Antos Laura García-Cansino María Ángeles García Dobrochna Ginter-Kramarczyk María Luisa Marina Joanna Zembrzuska José S. Câmara Jorge A. M. Pereira Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography Communications Chemistry |
| title | Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography |
| title_full | Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography |
| title_fullStr | Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography |
| title_full_unstemmed | Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography |
| title_short | Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography |
| title_sort | improved methodology to survey veterinary antibiotics in environmental samples using µspeed microextraction followed by ultraperformance liquid chromatography |
| url | https://doi.org/10.1038/s42004-025-01454-w |
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