Integrative multi-omics module network inference with Lemon-Tree.

Module network inference is an established statistical method to reconstruct co-expression modules and their upstream regulatory programs from integrated multi-omics datasets measuring the activity levels of various cellular components across different individuals, experimental conditions or time po...

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Main Authors: Eric Bonnet, Laurence Calzone, Tom Michoel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-02-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1003983
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author Eric Bonnet
Laurence Calzone
Tom Michoel
author_facet Eric Bonnet
Laurence Calzone
Tom Michoel
author_sort Eric Bonnet
collection DOAJ
description Module network inference is an established statistical method to reconstruct co-expression modules and their upstream regulatory programs from integrated multi-omics datasets measuring the activity levels of various cellular components across different individuals, experimental conditions or time points of a dynamic process. We have developed Lemon-Tree, an open-source, platform-independent, modular, extensible software package implementing state-of-the-art ensemble methods for module network inference. We benchmarked Lemon-Tree using large-scale tumor datasets and showed that Lemon-Tree algorithms compare favorably with state-of-the-art module network inference software. We also analyzed a large dataset of somatic copy-number alterations and gene expression levels measured in glioblastoma samples from The Cancer Genome Atlas and found that Lemon-Tree correctly identifies known glioblastoma oncogenes and tumor suppressors as master regulators in the inferred module network. Novel candidate driver genes predicted by Lemon-Tree were validated using tumor pathway and survival analyses. Lemon-Tree is available from http://lemon-tree.googlecode.com under the GNU General Public License version 2.0.
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spelling doaj-art-d20232e2f2ff4bbda3d549a32fabbbbf2025-08-20T02:34:06ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582015-02-01112e100398310.1371/journal.pcbi.1003983Integrative multi-omics module network inference with Lemon-Tree.Eric BonnetLaurence CalzoneTom MichoelModule network inference is an established statistical method to reconstruct co-expression modules and their upstream regulatory programs from integrated multi-omics datasets measuring the activity levels of various cellular components across different individuals, experimental conditions or time points of a dynamic process. We have developed Lemon-Tree, an open-source, platform-independent, modular, extensible software package implementing state-of-the-art ensemble methods for module network inference. We benchmarked Lemon-Tree using large-scale tumor datasets and showed that Lemon-Tree algorithms compare favorably with state-of-the-art module network inference software. We also analyzed a large dataset of somatic copy-number alterations and gene expression levels measured in glioblastoma samples from The Cancer Genome Atlas and found that Lemon-Tree correctly identifies known glioblastoma oncogenes and tumor suppressors as master regulators in the inferred module network. Novel candidate driver genes predicted by Lemon-Tree were validated using tumor pathway and survival analyses. Lemon-Tree is available from http://lemon-tree.googlecode.com under the GNU General Public License version 2.0.https://doi.org/10.1371/journal.pcbi.1003983
spellingShingle Eric Bonnet
Laurence Calzone
Tom Michoel
Integrative multi-omics module network inference with Lemon-Tree.
PLoS Computational Biology
title Integrative multi-omics module network inference with Lemon-Tree.
title_full Integrative multi-omics module network inference with Lemon-Tree.
title_fullStr Integrative multi-omics module network inference with Lemon-Tree.
title_full_unstemmed Integrative multi-omics module network inference with Lemon-Tree.
title_short Integrative multi-omics module network inference with Lemon-Tree.
title_sort integrative multi omics module network inference with lemon tree
url https://doi.org/10.1371/journal.pcbi.1003983
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AT laurencecalzone integrativemultiomicsmodulenetworkinferencewithlemontree
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