Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection
Umami and sweet taste detection is mediated by the activation of the TAS1R1/TAS1R3 and TAS1R2/TAS1R3 receptors, respectively. TAS1R2-Venus flytrap domain (VFT) constitutes the primary ligand-binding site for most of the sweeteners whereas TAS1R1-VFT contains the orthosteric binding site for umami co...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000073 |
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| author | Christine Belloir Lucie Moitrier Adeline Karolkowski Nicolas Poirier Fabrice Neiers Loïc Briand |
| author_facet | Christine Belloir Lucie Moitrier Adeline Karolkowski Nicolas Poirier Fabrice Neiers Loïc Briand |
| author_sort | Christine Belloir |
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| description | Umami and sweet taste detection is mediated by the activation of the TAS1R1/TAS1R3 and TAS1R2/TAS1R3 receptors, respectively. TAS1R2-Venus flytrap domain (VFT) constitutes the primary ligand-binding site for most of the sweeteners whereas TAS1R1-VFT contains the orthosteric binding site for umami compounds. Inosine-5′-monophosphate (IMP), previously known to potentiate umami taste, binds to a site of TAS1R1-VFT adjacent to the L-glutamate site leading to umami synergy. However, the involvement of the TAS1R3 subunit in umami receptor-ligand interactions or in synergy with IMP has never been demonstrated. To elucidate the VFT contribution to umami and sweet detection, we expressed human TAS1R1- and TAS1R3-VFTs in bacteria. Ligand binding studies quantified by intrinsic tryptophan fluorescence revealed that both TAS1R1- and TAS1R3-VFTs are able to interact with umami compounds. Cellular assays revealed that IMP is able, like cyclamate, to modulate the response of TAS1R2/TAS1R3 and TAS1R3 alone stimulated by calcium ions. IMP also acted as an enhancer of TAS1R2/TAS1R3 when stimulated with sucralose, neotame and cyclamate. Taking together, our data demonstrated that IMP modulates sweet compound detection at the receptor level acting via the TAS1R3 subunit. This research suggests more complex receptor interactions between umami and sweet taste qualities and paves the way for development of new sweetness enhancers. |
| format | Article |
| id | doaj-art-d1fbde1c2762401cbac133a91fc1ea16 |
| institution | Kabale University |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-d1fbde1c2762401cbac133a91fc1ea162025-08-20T03:45:28ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-011010024610.1016/j.fochms.2025.100246Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detectionChristine Belloir0Lucie Moitrier1Adeline Karolkowski2Nicolas Poirier3Fabrice Neiers4Loïc Briand5Corresponding authors.; Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceCorresponding authors.; Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Europe, F-21000 Dijon, FranceUmami and sweet taste detection is mediated by the activation of the TAS1R1/TAS1R3 and TAS1R2/TAS1R3 receptors, respectively. TAS1R2-Venus flytrap domain (VFT) constitutes the primary ligand-binding site for most of the sweeteners whereas TAS1R1-VFT contains the orthosteric binding site for umami compounds. Inosine-5′-monophosphate (IMP), previously known to potentiate umami taste, binds to a site of TAS1R1-VFT adjacent to the L-glutamate site leading to umami synergy. However, the involvement of the TAS1R3 subunit in umami receptor-ligand interactions or in synergy with IMP has never been demonstrated. To elucidate the VFT contribution to umami and sweet detection, we expressed human TAS1R1- and TAS1R3-VFTs in bacteria. Ligand binding studies quantified by intrinsic tryptophan fluorescence revealed that both TAS1R1- and TAS1R3-VFTs are able to interact with umami compounds. Cellular assays revealed that IMP is able, like cyclamate, to modulate the response of TAS1R2/TAS1R3 and TAS1R3 alone stimulated by calcium ions. IMP also acted as an enhancer of TAS1R2/TAS1R3 when stimulated with sucralose, neotame and cyclamate. Taking together, our data demonstrated that IMP modulates sweet compound detection at the receptor level acting via the TAS1R3 subunit. This research suggests more complex receptor interactions between umami and sweet taste qualities and paves the way for development of new sweetness enhancers.http://www.sciencedirect.com/science/article/pii/S2666566225000073G-protein-coupled receptorTaste receptorSweetenerUmamiRibonucleotide |
| spellingShingle | Christine Belloir Lucie Moitrier Adeline Karolkowski Nicolas Poirier Fabrice Neiers Loïc Briand Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection Food Chemistry: Molecular Sciences G-protein-coupled receptor Taste receptor Sweetener Umami Ribonucleotide |
| title | Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection |
| title_full | Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection |
| title_fullStr | Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection |
| title_full_unstemmed | Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection |
| title_short | Inosine-5′-monophosphate interacts with the TAS1R3 subunit to enhance sweet taste detection |
| title_sort | inosine 5 monophosphate interacts with the tas1r3 subunit to enhance sweet taste detection |
| topic | G-protein-coupled receptor Taste receptor Sweetener Umami Ribonucleotide |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000073 |
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