<i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration
<b>Background/Objectives</b>: <i>Corema album</i> berries are edible fruits from the Iberian Atlantic coast, characterized by a rich polyphenolic composition, which endows their juice with potential protective effects against neurodegeneration. This study aimed to evaluate th...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/17/11/1535 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850267166763384832 |
|---|---|
| author | Antonio Canoyra Carmen Martín-Cordero Dolores Muñoz-Mingarro Antonio J. León-González Richard B. Parsons Nuria Acero |
| author_facet | Antonio Canoyra Carmen Martín-Cordero Dolores Muñoz-Mingarro Antonio J. León-González Richard B. Parsons Nuria Acero |
| author_sort | Antonio Canoyra |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: <i>Corema album</i> berries are edible fruits from the Iberian Atlantic coast, characterized by a rich polyphenolic composition, which endows their juice with potential protective effects against neurodegeneration. This study aimed to evaluate the potential of the relatively lesser-known <i>C. album</i> berries as a novel neuroprotective agent against neurodegenerative diseases. <b>Methods</b>: The phenolic compounds of the juice were characterized using UHPLC-HRMS (Orbitrap). The SH-SY5Y neuroblastoma line was used to determine the preventive effect of the juice against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Furthermore, neuronal cells were differentiated into dopaminergic and cholinergic lines and exposed to 6-hydroxydopamine and okadaic acid, respectively, to simulate in vitro models of Parkinson’s disease and Alzheimer’s disease. The ability of the juice to enhance neuronal viability under toxic conditions was examined. Additionally, its inhibitory effects on neuroprotective-related enzymes, including MAO-A and MAO-B, were assessed in vitro. <b>Results:</b> Phytochemical characterization reveals that 5-<i>O</i>-caffeoylquinic acid constitutes 80% of the total phenolic compounds. Higher concentrations of the juice effectively protected both differentiated and undifferentiated SH-SY5Y cells from H<sub>2</sub>O<sub>2</sub>-induced oxidative damage, reducing oxidative stress by approximately 20% and suggesting a dose-dependent mechanism. Moreover, the presence of the juice significantly enhanced the viability of dopaminergic and cholinergic cells exposed to neurotoxic agents. In vitro, the juice inhibited the activity of MAO-A (IC<sub>50</sub> = 87.21 µg/mL) and MAO-B (IC<sub>50</sub> = 56.50 µg/mL). <b>Conclusions</b>: While these findings highlight <i>C. album</i> berries as a promising neuroprotective agent, further research is required to elucidate its neuroprotective mechanisms in cell and animal models and, ultimately, in human trials. |
| format | Article |
| id | doaj-art-d1f50cfc5a7d4e1fa82cf727de734445 |
| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-d1f50cfc5a7d4e1fa82cf727de7344452025-08-20T01:53:54ZengMDPI AGPharmaceuticals1424-82472024-11-011711153510.3390/ph17111535<i>Corema album</i> Berry Juice as a Protective Agent Against NeurodegenerationAntonio Canoyra0Carmen Martín-Cordero1Dolores Muñoz-Mingarro2Antonio J. León-González3Richard B. Parsons4Nuria Acero5Pharmaceutical and Health Science Department, Pharmacy Faculty, San Pablo-CEU University, CEU Universities, Urbanización Montepríncipe Boadilla del Monte, 28660 Madrid, SpainDepartment of Pharmacology, Faculty of Pharmacy, University of Seville, C/P. García González, 2, 41012 Seville, SpainChemistry and Biochemistry Department, Pharmacy Faculty, San Pablo-CEU University, CEU Universities, Urbanización Montepríncipe, Boadilla del Monte, 28668 Madrid, SpainDepartment of Pharmacology, Faculty of Pharmacy, University of Seville, C/P. García González, 2, 41012 Seville, SpainKing’s College London, Institute of Pharmaceutical Sciences, 150 Stamford Street, London SE1 9NH, UKPharmaceutical and Health Science Department, Pharmacy Faculty, San Pablo-CEU University, CEU Universities, Urbanización Montepríncipe Boadilla del Monte, 28660 Madrid, Spain<b>Background/Objectives</b>: <i>Corema album</i> berries are edible fruits from the Iberian Atlantic coast, characterized by a rich polyphenolic composition, which endows their juice with potential protective effects against neurodegeneration. This study aimed to evaluate the potential of the relatively lesser-known <i>C. album</i> berries as a novel neuroprotective agent against neurodegenerative diseases. <b>Methods</b>: The phenolic compounds of the juice were characterized using UHPLC-HRMS (Orbitrap). The SH-SY5Y neuroblastoma line was used to determine the preventive effect of the juice against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Furthermore, neuronal cells were differentiated into dopaminergic and cholinergic lines and exposed to 6-hydroxydopamine and okadaic acid, respectively, to simulate in vitro models of Parkinson’s disease and Alzheimer’s disease. The ability of the juice to enhance neuronal viability under toxic conditions was examined. Additionally, its inhibitory effects on neuroprotective-related enzymes, including MAO-A and MAO-B, were assessed in vitro. <b>Results:</b> Phytochemical characterization reveals that 5-<i>O</i>-caffeoylquinic acid constitutes 80% of the total phenolic compounds. Higher concentrations of the juice effectively protected both differentiated and undifferentiated SH-SY5Y cells from H<sub>2</sub>O<sub>2</sub>-induced oxidative damage, reducing oxidative stress by approximately 20% and suggesting a dose-dependent mechanism. Moreover, the presence of the juice significantly enhanced the viability of dopaminergic and cholinergic cells exposed to neurotoxic agents. In vitro, the juice inhibited the activity of MAO-A (IC<sub>50</sub> = 87.21 µg/mL) and MAO-B (IC<sub>50</sub> = 56.50 µg/mL). <b>Conclusions</b>: While these findings highlight <i>C. album</i> berries as a promising neuroprotective agent, further research is required to elucidate its neuroprotective mechanisms in cell and animal models and, ultimately, in human trials.https://www.mdpi.com/1424-8247/17/11/1535<i>Corema album</i>oxidative stressneuroprotectiveberriesphenolsAlzheimer’s disease |
| spellingShingle | Antonio Canoyra Carmen Martín-Cordero Dolores Muñoz-Mingarro Antonio J. León-González Richard B. Parsons Nuria Acero <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration Pharmaceuticals <i>Corema album</i> oxidative stress neuroprotective berries phenols Alzheimer’s disease |
| title | <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration |
| title_full | <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration |
| title_fullStr | <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration |
| title_full_unstemmed | <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration |
| title_short | <i>Corema album</i> Berry Juice as a Protective Agent Against Neurodegeneration |
| title_sort | i corema album i berry juice as a protective agent against neurodegeneration |
| topic | <i>Corema album</i> oxidative stress neuroprotective berries phenols Alzheimer’s disease |
| url | https://www.mdpi.com/1424-8247/17/11/1535 |
| work_keys_str_mv | AT antoniocanoyra icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration AT carmenmartincordero icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration AT doloresmunozmingarro icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration AT antoniojleongonzalez icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration AT richardbparsons icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration AT nuriaacero icoremaalbumiberryjuiceasaprotectiveagentagainstneurodegeneration |