Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification with Xylenol Orange Targeting Nucleocapsid Gene for Detection of Feline Coronavirus Infection

Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification with Xylenol Orange Targeting Nucleocapsid Gene for Detection of Feline Coronavirus Infection

Feline infectious peritonitis (FIP), a devastating disease with near-complete mortality, is caused by the feline coronavirus (FCoV) and affects domestic cats worldwide. Herein, we report the development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay incorporating x...

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Bibliographic Details
Main Authors: Kotchaporn Khumtong, Witsanu Rapichai, Wichayet Saejung, Piyamat Khamsingnok, Nianrawan Meecharoen, Siriluk Ratanabunyong, Hieu Van Dong, Supansa Tuanthap, Amonpun Rattanasrisomporn, Kiattawee Choowongkomon, Oumaporn Rungsuriyawiboon, Jatuporn Rattanasrisomporn
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Viruses
Subjects:
feline corona virus
FIP
RT-LAMP
Xylenol orange
qPCR
Online Access:https://www.mdpi.com/1999-4915/17/3/418
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