Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae
The production of metabolic energy in form of ATP by oxidative phosphorylation depends on the coordinated action of hundreds of nuclear-encoded mitochondrial proteins and a handful of proteins encoded by the mitochondrial genome (mtDNA). We used the yeast Saccharomyces cerevisiae as a model system t...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2020-06-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2020a-stenger-microbial-cell/ |
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| author | Maria Stenger Duc Tung Le Till Klecker Benedikt Westermann |
| author_facet | Maria Stenger Duc Tung Le Till Klecker Benedikt Westermann |
| author_sort | Maria Stenger |
| collection | DOAJ |
| description | The production of metabolic energy in form of ATP by oxidative phosphorylation depends on the coordinated action of hundreds of nuclear-encoded mitochondrial proteins and a handful of proteins encoded by the mitochondrial genome (mtDNA). We used the yeast Saccharomyces cerevisiae as a model system to systematically identify the genes contributing to this process. Integration of genome-wide high-throughput growth assays with previously published large data sets allowed us to define with high confidence a set of 254 nuclear genes that are indispensable for respiratory growth. Next, we induced loss of mtDNA in the yeast deletion collection by growth on ethidium bromide-containing medium and identified twelve genes that are essential for viability in the absence of mtDNA (i.e. petite-negative). Replenishment of mtDNA by cytoduction showed that respiratory-deficient phenotypes are highly variable in many yeast mutants. Using a mitochondrial genome carrying a selectable marker, ARG8m, we screened for mutants that are specifically defective in maintenance of mtDNA and mitochondrial protein synthesis. We found that up to 176 nuclear genes are required for expression of mitochondria-encoded proteins during fermentative growth. Taken together, our data provide a comprehensive picture of the molecular processes that are required for respiratory metabolism in a simple eukaryotic cell. |
| format | Article |
| id | doaj-art-d1dee1ef25ed49b3bbac0ad1e68f631e |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-d1dee1ef25ed49b3bbac0ad1e68f631e2025-08-20T02:57:37ZengShared Science Publishers OGMicrobial Cell2311-26382020-06-017923424910.15698/mic2020.09.729Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiaeMaria Stenger0Duc Tung Le1Till Klecker2Benedikt Westermann3Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany. Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany. Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany. Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany. The production of metabolic energy in form of ATP by oxidative phosphorylation depends on the coordinated action of hundreds of nuclear-encoded mitochondrial proteins and a handful of proteins encoded by the mitochondrial genome (mtDNA). We used the yeast Saccharomyces cerevisiae as a model system to systematically identify the genes contributing to this process. Integration of genome-wide high-throughput growth assays with previously published large data sets allowed us to define with high confidence a set of 254 nuclear genes that are indispensable for respiratory growth. Next, we induced loss of mtDNA in the yeast deletion collection by growth on ethidium bromide-containing medium and identified twelve genes that are essential for viability in the absence of mtDNA (i.e. petite-negative). Replenishment of mtDNA by cytoduction showed that respiratory-deficient phenotypes are highly variable in many yeast mutants. Using a mitochondrial genome carrying a selectable marker, ARG8m, we screened for mutants that are specifically defective in maintenance of mtDNA and mitochondrial protein synthesis. We found that up to 176 nuclear genes are required for expression of mitochondria-encoded proteins during fermentative growth. Taken together, our data provide a comprehensive picture of the molecular processes that are required for respiratory metabolism in a simple eukaryotic cell.http://microbialcell.com/researcharticles/2020a-stenger-microbial-cell/mitochondriamitochondrial dnaoxidative phosphorylationpetite mutantyeast |
| spellingShingle | Maria Stenger Duc Tung Le Till Klecker Benedikt Westermann Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae Microbial Cell mitochondria mitochondrial dna oxidative phosphorylation petite mutant yeast |
| title | Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae |
| title_full | Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae |
| title_fullStr | Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae |
| title_full_unstemmed | Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae |
| title_short | Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in S. cerevisiae |
| title_sort | systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome in s cerevisiae |
| topic | mitochondria mitochondrial dna oxidative phosphorylation petite mutant yeast |
| url | http://microbialcell.com/researcharticles/2020a-stenger-microbial-cell/ |
| work_keys_str_mv | AT mariastenger systematicanalysisofnucleargenefunctioninrespiratorygrowthandexpressionofthemitochondrialgenomeinscerevisiae AT ductungle systematicanalysisofnucleargenefunctioninrespiratorygrowthandexpressionofthemitochondrialgenomeinscerevisiae AT tillklecker systematicanalysisofnucleargenefunctioninrespiratorygrowthandexpressionofthemitochondrialgenomeinscerevisiae AT benediktwestermann systematicanalysisofnucleargenefunctioninrespiratorygrowthandexpressionofthemitochondrialgenomeinscerevisiae |