Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin
The spatial–temporal dynamics of an in vitro radicular system of <i>Typha domingensis</i> for the development of rhizofiltration technologies, with the potential for use as a phytotreatment of eutrophicated water, were studied for the first time in the roots of seedlings and in rhizotron...
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2025-01-01
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| author | Guadalupe Hernández-Piedra Violeta Ruiz-Carrera Alberto J. Sánchez Erika Escalante-Espinosa Graciano Calva-Calva |
| author_facet | Guadalupe Hernández-Piedra Violeta Ruiz-Carrera Alberto J. Sánchez Erika Escalante-Espinosa Graciano Calva-Calva |
| author_sort | Guadalupe Hernández-Piedra |
| collection | DOAJ |
| description | The spatial–temporal dynamics of an in vitro radicular system of <i>Typha domingensis</i> for the development of rhizofiltration technologies, with the potential for use as a phytotreatment of eutrophicated water, were studied for the first time in the roots of seedlings and in rhizotron systems. The effect of indole-3-acetic acid (AIA) in combination with kinetin (CIN) or 6-benzylaminopurine (BAP) on seedlings cultivated in the light and dark in three radicular systems and in a rhizotrophic regime for the screening of dynamic rhizogenic lines, by weekly allometric measurements of the length and number of roots, were studied. Inhibition of the elongation and branching velocities of roots by BAP and light was observed but CIN increased elongation and branching. In rhizotrons cultivated in light and dark conditions with different AIA/CIN ratios, isolated root explants remained inactive; however, roots attached to a meristematic base presented a significant increase in growth development, with values comparable to those of roots attached to seedlings cultivated in light without hormones. The results revealed that six adventitious rhizogenic root lines with basal meristems have the potential for use in a wide range of environmental and innovative applications in phytotreatment technologies involving eutrophicated water. |
| format | Article |
| id | doaj-art-d1dc43d6a6e947c29b33b0a44f150887 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-d1dc43d6a6e947c29b33b0a44f1508872025-01-24T13:38:52ZengMDPI AGLife2075-17292025-01-0115112110.3390/life15010121Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/CytokininGuadalupe Hernández-Piedra0Violeta Ruiz-Carrera1Alberto J. Sánchez2Erika Escalante-Espinosa3Graciano Calva-Calva4Programa de Doctorado en Ecología y Manejo de Sistemas Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, MexicoDiagnóstico y Manejo de Humedales Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, MexicoDiagnóstico y Manejo de Humedales Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, MexicoDiagnóstico y Manejo de Humedales Tropicales, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5 S/N Entronque a Bosques de Saloya, Villahermosa C.P. 86150, Tabasco, MexicoBiotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del IPN, Avenida Instituto Politécnico Nacional 2508, Colonia San Pedro Zacatenco, Ciudad de México C.P. 07360, MexicoThe spatial–temporal dynamics of an in vitro radicular system of <i>Typha domingensis</i> for the development of rhizofiltration technologies, with the potential for use as a phytotreatment of eutrophicated water, were studied for the first time in the roots of seedlings and in rhizotron systems. The effect of indole-3-acetic acid (AIA) in combination with kinetin (CIN) or 6-benzylaminopurine (BAP) on seedlings cultivated in the light and dark in three radicular systems and in a rhizotrophic regime for the screening of dynamic rhizogenic lines, by weekly allometric measurements of the length and number of roots, were studied. Inhibition of the elongation and branching velocities of roots by BAP and light was observed but CIN increased elongation and branching. In rhizotrons cultivated in light and dark conditions with different AIA/CIN ratios, isolated root explants remained inactive; however, roots attached to a meristematic base presented a significant increase in growth development, with values comparable to those of roots attached to seedlings cultivated in light without hormones. The results revealed that six adventitious rhizogenic root lines with basal meristems have the potential for use in a wide range of environmental and innovative applications in phytotreatment technologies involving eutrophicated water.https://www.mdpi.com/2075-1729/15/1/121Typhaceaein vitro culturerooting dynamicsrhizotron |
| spellingShingle | Guadalupe Hernández-Piedra Violeta Ruiz-Carrera Alberto J. Sánchez Erika Escalante-Espinosa Graciano Calva-Calva Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin Life Typhaceae in vitro culture rooting dynamics rhizotron |
| title | Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin |
| title_full | Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin |
| title_fullStr | Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin |
| title_full_unstemmed | Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin |
| title_short | Spatial–Temporal Dynamics of Adventitious Roots of <i>Typha domingensis</i> Pers. Seedlings Grown with Auxin/Cytokinin |
| title_sort | spatial temporal dynamics of adventitious roots of i typha domingensis i pers seedlings grown with auxin cytokinin |
| topic | Typhaceae in vitro culture rooting dynamics rhizotron |
| url | https://www.mdpi.com/2075-1729/15/1/121 |
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