Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats
The present study examined the dose-dependent effect of vitamin E in reversing bone loss in ovariectomized (Ovx) rats. Sprague-Dawley rats were either Sham-operated (Sham) or Ovx and fed control diet for 120 days to lose bone. Subsequently, rats were divided into 5 groups (n=12/group): Sham, Ovx-con...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Osteoporosis |
| Online Access: | http://dx.doi.org/10.1155/2013/825985 |
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| author | Rafaela G. Feresin Sarah A. Johnson Marcus L. Elam Jeong-Su Kim Dania A. Khalil Edralin A. Lucas Brenda J. Smith Mark E. Payton Mohammed P. Akhter Bahram H. Arjmandi |
| author_facet | Rafaela G. Feresin Sarah A. Johnson Marcus L. Elam Jeong-Su Kim Dania A. Khalil Edralin A. Lucas Brenda J. Smith Mark E. Payton Mohammed P. Akhter Bahram H. Arjmandi |
| author_sort | Rafaela G. Feresin |
| collection | DOAJ |
| description | The present study examined the dose-dependent effect of vitamin E in reversing bone loss in ovariectomized (Ovx) rats. Sprague-Dawley rats were either Sham-operated (Sham) or Ovx and fed control diet for 120 days to lose bone. Subsequently, rats were divided into 5 groups (n=12/group): Sham, Ovx-control, low dose (Ovx + 300 mg/kg diet; LD), medium dose (Ovx + 525 mg/kg diet; MD), and high dose (Ovx + 750 mg/kg diet; HD) of vitamin E and sacrificed after 100 days. Animals receiving MD and HD of vitamin E had increased serum alkaline phosphatase compared to the Ovx-control group. Bone histomorphometry analysis indicated a decrease in bone resorption as well as increased bone formation and mineralization in the Ovx groups supplemented with MD and HD of vitamin E. Microcomputed tomography findings indicated no effects of vitamin E on trabecular bone of fifth lumbar vertebrae. Animals receiving HD of vitamin E had enhanced fourth lumbar vertebra quality as evidenced by improved ultimate and yield load and stress when compared to Ovx-control group. These findings demonstrate that vitamin E improves bone quality, attenuates bone resorption, and enhances the rate of bone formation while being unable to restore bone density and trabecular bone structure. |
| format | Article |
| id | doaj-art-d1dbf66c82fe4f86bd038caa76fa1408 |
| institution | DOAJ |
| issn | 2090-8059 2042-0064 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Osteoporosis |
| spelling | doaj-art-d1dbf66c82fe4f86bd038caa76fa14082025-08-20T03:24:02ZengWileyJournal of Osteoporosis2090-80592042-00642013-01-01201310.1155/2013/825985825985Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized RatsRafaela G. Feresin0Sarah A. Johnson1Marcus L. Elam2Jeong-Su Kim3Dania A. Khalil4Edralin A. Lucas5Brenda J. Smith6Mark E. Payton7Mohammed P. Akhter8Bahram H. Arjmandi9Nutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USANutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USANutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USANutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USANutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USADepartments of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartments of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartments of Statistics, Oklahoma State University, Stillwater, OK 74078, USAOsteoporosis Research Center, Creighton University, Omaha, NE 68131, USANutrition, Food & Exercise Sciences, The Florida State University, Tallahassee, FL 32306, USAThe present study examined the dose-dependent effect of vitamin E in reversing bone loss in ovariectomized (Ovx) rats. Sprague-Dawley rats were either Sham-operated (Sham) or Ovx and fed control diet for 120 days to lose bone. Subsequently, rats were divided into 5 groups (n=12/group): Sham, Ovx-control, low dose (Ovx + 300 mg/kg diet; LD), medium dose (Ovx + 525 mg/kg diet; MD), and high dose (Ovx + 750 mg/kg diet; HD) of vitamin E and sacrificed after 100 days. Animals receiving MD and HD of vitamin E had increased serum alkaline phosphatase compared to the Ovx-control group. Bone histomorphometry analysis indicated a decrease in bone resorption as well as increased bone formation and mineralization in the Ovx groups supplemented with MD and HD of vitamin E. Microcomputed tomography findings indicated no effects of vitamin E on trabecular bone of fifth lumbar vertebrae. Animals receiving HD of vitamin E had enhanced fourth lumbar vertebra quality as evidenced by improved ultimate and yield load and stress when compared to Ovx-control group. These findings demonstrate that vitamin E improves bone quality, attenuates bone resorption, and enhances the rate of bone formation while being unable to restore bone density and trabecular bone structure.http://dx.doi.org/10.1155/2013/825985 |
| spellingShingle | Rafaela G. Feresin Sarah A. Johnson Marcus L. Elam Jeong-Su Kim Dania A. Khalil Edralin A. Lucas Brenda J. Smith Mark E. Payton Mohammed P. Akhter Bahram H. Arjmandi Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats Journal of Osteoporosis |
| title | Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats |
| title_full | Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats |
| title_fullStr | Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats |
| title_full_unstemmed | Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats |
| title_short | Effects of Vitamin E on Bone Biomechanical and Histomorphometric Parameters in Ovariectomized Rats |
| title_sort | effects of vitamin e on bone biomechanical and histomorphometric parameters in ovariectomized rats |
| url | http://dx.doi.org/10.1155/2013/825985 |
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