High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China
Soybean, an essential oil crop in China, has witnessed accelerated seed transfer domestically and abroad in recent years. Seed carriage has emerged as a major route for the dissemination of soybean diseases. In this study, 14 soybean cultivars from three northeastern provinces were collected and exa...
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MDPI AG
2025-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/2/436 |
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| author | Yuanyuan Wang Qingyao Bai Fanqi Meng Wei Dong Haiyan Fan Xiaofeng Zhu Yuxi Duan Lijie Chen |
| author_facet | Yuanyuan Wang Qingyao Bai Fanqi Meng Wei Dong Haiyan Fan Xiaofeng Zhu Yuxi Duan Lijie Chen |
| author_sort | Yuanyuan Wang |
| collection | DOAJ |
| description | Soybean, an essential oil crop in China, has witnessed accelerated seed transfer domestically and abroad in recent years. Seed carriage has emerged as a major route for the dissemination of soybean diseases. In this study, 14 soybean cultivars from three northeastern provinces were collected and examined for seed-borne microorganisms using traditional detection technology and high-throughput sequencing technology. Through traditional detection techniques, a total of six genera of bacteria and seventeen genera of fungi were isolated from the test varieties. The quantity and types of microorganisms on the seed surface were greater than those on the seed coat and within the seed, while the seed coat and internal seed contained fewer microorganisms. The dominant fungal genera were <i>Cladosporium</i>, <i>Fusarium</i>, <i>Aspergillus</i>, and <i>Alternaria</i>, accounting for 21.23%, 17.45%, 15.57%, and 11.56% of the genera, respectively. The dominant bacterial genera were <i>Pseudomonas</i>, <i>Sphingomonas</i>, and <i>Pantoea</i>, accounting for 37.46%, 17.29%, and 15.27% of the genera, respectively. The dominant genera obtained through traditional seed-carrying assay techniques were also dominant in high-throughput sequencing. However, some dominant genera obtained through high-throughput sequencing were not isolated by traditional methods. High-throughput sequencing analysis revealed that soybean seeds from Jilin Province had the highest abundance of seed-borne fungi, followed by seeds from Liaoning Province and Heilongjiang Province. Jilin Province also had the highest abundance of seed-borne bacteria, followed by Heilongjiang Province and Liaoning Province. The isolation and identification of microorganisms on soybean seeds provide a scientific basis for seed quarantine treatment and disease control, which is of great significance for soybean production in China. |
| format | Article |
| id | doaj-art-d1d5a1ca028344a5bb77d7b9937271ef |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-d1d5a1ca028344a5bb77d7b9937271ef2025-08-20T03:11:07ZengMDPI AGAgronomy2073-43952025-02-0115243610.3390/agronomy15020436High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast ChinaYuanyuan Wang0Qingyao Bai1Fanqi Meng2Wei Dong3Haiyan Fan4Xiaofeng Zhu5Yuxi Duan6Lijie Chen7College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, ChinaNematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, ChinaNematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, ChinaNematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, ChinaNematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, ChinaNematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, ChinaSoybean, an essential oil crop in China, has witnessed accelerated seed transfer domestically and abroad in recent years. Seed carriage has emerged as a major route for the dissemination of soybean diseases. In this study, 14 soybean cultivars from three northeastern provinces were collected and examined for seed-borne microorganisms using traditional detection technology and high-throughput sequencing technology. Through traditional detection techniques, a total of six genera of bacteria and seventeen genera of fungi were isolated from the test varieties. The quantity and types of microorganisms on the seed surface were greater than those on the seed coat and within the seed, while the seed coat and internal seed contained fewer microorganisms. The dominant fungal genera were <i>Cladosporium</i>, <i>Fusarium</i>, <i>Aspergillus</i>, and <i>Alternaria</i>, accounting for 21.23%, 17.45%, 15.57%, and 11.56% of the genera, respectively. The dominant bacterial genera were <i>Pseudomonas</i>, <i>Sphingomonas</i>, and <i>Pantoea</i>, accounting for 37.46%, 17.29%, and 15.27% of the genera, respectively. The dominant genera obtained through traditional seed-carrying assay techniques were also dominant in high-throughput sequencing. However, some dominant genera obtained through high-throughput sequencing were not isolated by traditional methods. High-throughput sequencing analysis revealed that soybean seeds from Jilin Province had the highest abundance of seed-borne fungi, followed by seeds from Liaoning Province and Heilongjiang Province. Jilin Province also had the highest abundance of seed-borne bacteria, followed by Heilongjiang Province and Liaoning Province. The isolation and identification of microorganisms on soybean seeds provide a scientific basis for seed quarantine treatment and disease control, which is of great significance for soybean production in China.https://www.mdpi.com/2073-4395/15/2/436soybeanmicrobial diversityconventional seed-borne microorganism detection technologyhigh-throughput sequencing technology |
| spellingShingle | Yuanyuan Wang Qingyao Bai Fanqi Meng Wei Dong Haiyan Fan Xiaofeng Zhu Yuxi Duan Lijie Chen High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China Agronomy soybean microbial diversity conventional seed-borne microorganism detection technology high-throughput sequencing technology |
| title | High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China |
| title_full | High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China |
| title_fullStr | High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China |
| title_full_unstemmed | High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China |
| title_short | High-Throughput Sequence Analysis of Microbial Communities of Soybean in Northeast China |
| title_sort | high throughput sequence analysis of microbial communities of soybean in northeast china |
| topic | soybean microbial diversity conventional seed-borne microorganism detection technology high-throughput sequencing technology |
| url | https://www.mdpi.com/2073-4395/15/2/436 |
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