Triple-Parameter Optimization of Linezolid 600mg Tablets: Dissolution, HPLC, and UV Spectrophotometric Evaluation
Aims and Objectives: The current research’s target is to obtain the dissolution profile of various brands of Linezolid 600 mg tablets available in Karachi, Pakistan and the assay was also performed using both the UV Spectrophotometric Technique and High Performance Liquid Chromatographic Techniques...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Jinnah University for Women
2025-02-01
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| Series: | RADS Journal of Biological Research & Applied Science |
| Subjects: | |
| Online Access: | http://www.jbas.juw.edu.pk/index.php/JBAS/article/view/672 |
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| Summary: | Aims and Objectives: The current research’s target is to obtain the dissolution profile of various brands of Linezolid 600 mg tablets available in Karachi, Pakistan and the assay was also performed using both the UV Spectrophotometric Technique and High Performance Liquid Chromatographic Techniques.
Methodology: Dissolution profile was conducted using 0.05M phosphate buffer (pH 6.8). UV spectrophotometry and HPLC techniques were employed for assay. The mobile phases used were Methanol: Buffer (55:45) for HPLC and Acetonitrile: Water (50:50) for UV.
Results: The dissolution profiles showed that Brand C-1 had the fastest dissolution rate, achieving 100% dissolution within 20 minutes. The assay results showed that Brand C-1 had an HPLC assay value of 99.5% ± 0.84 and a UV assay value of 104.3% ± 0.14. Therefore, Brand C-1 can be considered as the best brand among the tested brands.
Conclusion: HPLC is recommended as the best analytical method for the estimation of Linezolid due to its high precision and accuracy. However, the UV spectrophotometric method is rather quick and affordable. Complex procedures or treatments (often associated with the chromatographic approach) do not require it. This study fills a research vacuum by comparing the dissolution profile and assay of various brands of 600 mg Linezolid tablets that are available in Karachi, Pakistan. By using both UV Spectrophotometric and High Performance Liquid Chromatographic (HPLC) methodologies, the study specifically aims to close the gap in assessing the bioequivalence and quality consistency of various brands.
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| ISSN: | 2305-8722 2521-8573 |