Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture
Cytogenetic bands reflect genomic organization in large blocks of DNA with similar properties. Because banding patterns are invariant, this organization may often be assumed unimportant for genome regulation. Results here challenge that view. Findings here suggest cytogenetic bands reflect a visible...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Nucleus |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19491034.2024.2400525 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850137940095664128 |
|---|---|
| author | Lisa L. Hall Kevin M. Creamer Meg Byron Jeanne B. Lawrence |
| author_facet | Lisa L. Hall Kevin M. Creamer Meg Byron Jeanne B. Lawrence |
| author_sort | Lisa L. Hall |
| collection | DOAJ |
| description | Cytogenetic bands reflect genomic organization in large blocks of DNA with similar properties. Because banding patterns are invariant, this organization may often be assumed unimportant for genome regulation. Results here challenge that view. Findings here suggest cytogenetic bands reflect a visible framework upon which regulated genome architecture is built. Given Alu and L1 densities differ in cytogenetic bands, we examined their distribution after X-chromosome inactivation or formation of senescent-associated heterochromatin foci (SAHFs). Alu-rich regions remain outside both SAHFs and the Barr Body (BB), affirming that the BB is not the whole chromosome but a condensed, L1-rich core. Hi-C analysis of senescent cells demonstrates large (~10 Mb) G-bands remodel as a contiguous unit, gaining distal intrachromosomal interactions as syntenic G-bands coalesce into SAHFs. Striking peaks of Alu within R-bands strongly resist condensation. Thus, large-scale segmental genome architectur relates to dark versus light cytogenetic bands and Alu-peaks, implicating both in chromatin regulation. |
| format | Article |
| id | doaj-art-d1ba3c936c7b4a0ba630189b2ca1017a |
| institution | OA Journals |
| issn | 1949-1034 1949-1042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj-art-d1ba3c936c7b4a0ba630189b2ca1017a2025-08-20T02:30:42ZengTaylor & Francis GroupNucleus1949-10341949-10422024-12-0115110.1080/19491034.2024.2400525Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architectureLisa L. Hall0Kevin M. Creamer1Meg Byron2Jeanne B. Lawrence3Department of Neurology, University of Massachusetts Chan Medical School, Worcester, MA, USADepartment of Neurology, University of Massachusetts Chan Medical School, Worcester, MA, USADepartment of Neurology, University of Massachusetts Chan Medical School, Worcester, MA, USADepartment of Neurology, University of Massachusetts Chan Medical School, Worcester, MA, USACytogenetic bands reflect genomic organization in large blocks of DNA with similar properties. Because banding patterns are invariant, this organization may often be assumed unimportant for genome regulation. Results here challenge that view. Findings here suggest cytogenetic bands reflect a visible framework upon which regulated genome architecture is built. Given Alu and L1 densities differ in cytogenetic bands, we examined their distribution after X-chromosome inactivation or formation of senescent-associated heterochromatin foci (SAHFs). Alu-rich regions remain outside both SAHFs and the Barr Body (BB), affirming that the BB is not the whole chromosome but a condensed, L1-rich core. Hi-C analysis of senescent cells demonstrates large (~10 Mb) G-bands remodel as a contiguous unit, gaining distal intrachromosomal interactions as syntenic G-bands coalesce into SAHFs. Striking peaks of Alu within R-bands strongly resist condensation. Thus, large-scale segmental genome architectur relates to dark versus light cytogenetic bands and Alu-peaks, implicating both in chromatin regulation.https://www.tandfonline.com/doi/10.1080/19491034.2024.2400525RepeatsLINE1AluX-inactivationsenescenceSAHFs |
| spellingShingle | Lisa L. Hall Kevin M. Creamer Meg Byron Jeanne B. Lawrence Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture Nucleus Repeats LINE1 Alu X-inactivation senescence SAHFs |
| title | Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture |
| title_full | Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture |
| title_fullStr | Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture |
| title_full_unstemmed | Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture |
| title_short | Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture |
| title_sort | cytogenetic bands and sharp peaks of alu underlie large scale segmental regulation of nuclear genome architecture |
| topic | Repeats LINE1 Alu X-inactivation senescence SAHFs |
| url | https://www.tandfonline.com/doi/10.1080/19491034.2024.2400525 |
| work_keys_str_mv | AT lisalhall cytogeneticbandsandsharppeaksofaluunderlielargescalesegmentalregulationofnucleargenomearchitecture AT kevinmcreamer cytogeneticbandsandsharppeaksofaluunderlielargescalesegmentalregulationofnucleargenomearchitecture AT megbyron cytogeneticbandsandsharppeaksofaluunderlielargescalesegmentalregulationofnucleargenomearchitecture AT jeanneblawrence cytogeneticbandsandsharppeaksofaluunderlielargescalesegmentalregulationofnucleargenomearchitecture |