Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice

Abstract Cortical descending modulation of sensory processing in the spinal cord has been revealed, the role of cortico-brainstem projections in sensory and motor regulation remains poorly investigated. Here, we identified two previously unappreciated subpopulations in the primary sensorimotor corte...

Full description

Saved in:
Bibliographic Details
Main Authors: Guo-Hong Wang, Xin-Yu Hou, Hui-Zhu Liu, Zi-Rui Zhou, Su-Su Lv, Lan-Xing Yi, Hui Li, Yu-Qiu Zhang
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61164-8
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849334646646505472
author Guo-Hong Wang
Xin-Yu Hou
Hui-Zhu Liu
Zi-Rui Zhou
Su-Su Lv
Lan-Xing Yi
Hui Li
Yu-Qiu Zhang
author_facet Guo-Hong Wang
Xin-Yu Hou
Hui-Zhu Liu
Zi-Rui Zhou
Su-Su Lv
Lan-Xing Yi
Hui Li
Yu-Qiu Zhang
author_sort Guo-Hong Wang
collection DOAJ
description Abstract Cortical descending modulation of sensory processing in the spinal cord has been revealed, the role of cortico-brainstem projections in sensory and motor regulation remains poorly investigated. Here, we identified two previously unappreciated subpopulations in the primary sensorimotor cortex (SM1), which are anatomically and electrophysiologically distinct but functionally similar, projecting to the periaqueductal gray (PAG) and rostroventral medulla (RVM), respectively. Postsynaptic PAG glutamatergic neurons then project to the noradrenergic (NA) neurons in the locus coeruleus (LC) and 5-HT neurons in the RVM to drive spinal NA and 5-HT anti-nociception, while RVM enkephalinergic neurons that receive SM1 projections directly drive spinal opioid analgesia. Intriguingly, silencing these two SM1 subpopulations impair motor behaviors and motor-induced analgesia, but does not affect behavioral responses to cutaneous sensory stimuli. Our findings indicate that these brainstem-projecting SM1 subpopulations are crucial for cortical descending pain modulation, which could be exploited to optimize pain relief approaches.
format Article
id doaj-art-d1b4aa56947e49ec8d8d7598346b65af
institution Kabale University
issn 2041-1723
language English
publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-d1b4aa56947e49ec8d8d7598346b65af2025-08-20T03:45:31ZengNature PortfolioNature Communications2041-17232025-07-0116111810.1038/s41467-025-61164-8Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in miceGuo-Hong Wang0Xin-Yu Hou1Hui-Zhu Liu2Zi-Rui Zhou3Su-Su Lv4Lan-Xing Yi5Hui Li6Yu-Qiu Zhang7Department of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityDepartment of Translational Neuroscience, Jing’an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan UniversityAbstract Cortical descending modulation of sensory processing in the spinal cord has been revealed, the role of cortico-brainstem projections in sensory and motor regulation remains poorly investigated. Here, we identified two previously unappreciated subpopulations in the primary sensorimotor cortex (SM1), which are anatomically and electrophysiologically distinct but functionally similar, projecting to the periaqueductal gray (PAG) and rostroventral medulla (RVM), respectively. Postsynaptic PAG glutamatergic neurons then project to the noradrenergic (NA) neurons in the locus coeruleus (LC) and 5-HT neurons in the RVM to drive spinal NA and 5-HT anti-nociception, while RVM enkephalinergic neurons that receive SM1 projections directly drive spinal opioid analgesia. Intriguingly, silencing these two SM1 subpopulations impair motor behaviors and motor-induced analgesia, but does not affect behavioral responses to cutaneous sensory stimuli. Our findings indicate that these brainstem-projecting SM1 subpopulations are crucial for cortical descending pain modulation, which could be exploited to optimize pain relief approaches.https://doi.org/10.1038/s41467-025-61164-8
spellingShingle Guo-Hong Wang
Xin-Yu Hou
Hui-Zhu Liu
Zi-Rui Zhou
Su-Su Lv
Lan-Xing Yi
Hui Li
Yu-Qiu Zhang
Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
Nature Communications
title Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
title_full Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
title_fullStr Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
title_full_unstemmed Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
title_short Descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
title_sort descending projection neurons in the primary sensorimotor cortex regulate neuropathic pain and locomotion in mice
url https://doi.org/10.1038/s41467-025-61164-8
work_keys_str_mv AT guohongwang descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT xinyuhou descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT huizhuliu descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT ziruizhou descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT susulv descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT lanxingyi descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT huili descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice
AT yuqiuzhang descendingprojectionneuronsintheprimarysensorimotorcortexregulateneuropathicpainandlocomotioninmice