Two-Step Enzymatic Synthesis of Structured Triacylglycerols from ARASCO<sup>©</sup> and Coconut Oil
Triacylglycerols containing medium-chain fatty acids at the <i>sn</i>-1,3 positions and a long-chain fatty acid at the <i>sn</i>-2 position (MLM-TAG) are of nutritional interest. However, they are scarce in common food sources and are usually synthesized by chemical or enzyma...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/14/24/11576 |
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| Summary: | Triacylglycerols containing medium-chain fatty acids at the <i>sn</i>-1,3 positions and a long-chain fatty acid at the <i>sn</i>-2 position (MLM-TAG) are of nutritional interest. However, they are scarce in common food sources and are usually synthesized by chemical or enzymatic methods. In this work, the enzymatic synthesis of MLM-TAG was attempted using <i>sn</i>-2 monoacylglycerols (<i>sn</i>-2 MAG) from the ethanolysis of an arachidonic acid-rich fraction from ARASCO and fatty acid ethyl esters from the ethanolysis of coconut oil as substrates. The highest yield of <i>sn</i>-2 MAG (23.3 mol%) was obtained after 1 h of ethanolysis with Novozym 435 lipase at 25 °C, and the best profile of the ethanolysis products of coconut oil was obtained after 24 h of reaction catalyzed by the lipase from <i>Thermomyces lanuginosus</i>. Regarding the enzymatic synthesis of structured TAG, the lipase from <i>Rhizopus oryzae</i> gave better results than those from <i>Thermomyces lanuginosus</i> and <i>Rhizomucor miehei</i>, with the <i>sn</i>-2 position mainly esterified with arachidonic acid (34.8%) and the <i>sn</i>-1,3 positions mainly esterified with capric and lauric acids (35.1%). This work focuses on a simple process for the enzymatic production of structured TAG without prior purification of the <i>sn</i>-2 MAG. |
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| ISSN: | 2076-3417 |