Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.

The incidence of End Stage Renal Disease (ESRD) is approximately 50% higher in men than women. In order to understand the molecular basis of this gender disparity, we examined sex specific gene expression patterns in control and diseased, human and murine kidney samples. Using the Affymetrix platfor...

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Main Authors: Han Si, Ramandeep S Banga, Pinelopi Kapitsinou, Manjunath Ramaiah, Janis Lawrence, Ganesh Kambhampati, Antje Gruenwald, Erwin Bottinger, Daniel Glicklich, Vivian Tellis, Stuart Greenstein, David B Thomas, James Pullman, Melissa Fazzari, Katalin Susztak
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004802&type=printable
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author Han Si
Ramandeep S Banga
Pinelopi Kapitsinou
Manjunath Ramaiah
Janis Lawrence
Ganesh Kambhampati
Antje Gruenwald
Erwin Bottinger
Daniel Glicklich
Vivian Tellis
Stuart Greenstein
David B Thomas
James Pullman
Melissa Fazzari
Katalin Susztak
author_facet Han Si
Ramandeep S Banga
Pinelopi Kapitsinou
Manjunath Ramaiah
Janis Lawrence
Ganesh Kambhampati
Antje Gruenwald
Erwin Bottinger
Daniel Glicklich
Vivian Tellis
Stuart Greenstein
David B Thomas
James Pullman
Melissa Fazzari
Katalin Susztak
author_sort Han Si
collection DOAJ
description The incidence of End Stage Renal Disease (ESRD) is approximately 50% higher in men than women. In order to understand the molecular basis of this gender disparity, we examined sex specific gene expression patterns in control and diseased, human and murine kidney samples. Using the Affymetrix platform we performed comprehensive gene expression analysis on 42 microdissected human kidney samples (glomeruli and tubules). We identified 67 genes with gender biased expression in healthy human kidneys and 24 transcripts in diseased male and female human kidneys. Similar analysis performed in mice using male and female control and doxorubicin induced nephrotic syndrome kidneys identified significantly larger number of differentially expressed transcripts. The majority of genes showing gender biased expression either in diseased human and murine kidneys were different from those differentially expressed in healthy kidneys. Only 9 sexually dimorphic transcripts were common to healthy human and murine kidneys and five showed differential regulation in both human and murine diseased kidneys. In humans, sex biased genes showed statistical enrichment only to sex chromosomes while in mice they were enriched to sex chromosomes and various autosomes. Thus we present a comprehensive analysis of gender biased genes in the kidney. We show that sexually dimorphic genes in the kidney show species specific regulation. Our results also indicate that male and female kidneys respond differently to injury. These studies could provide the basis for the development of new treatment strategies for men and women with kidney disease.
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spelling doaj-art-d197885fe9064557ac10bcaab1cda73c2025-08-20T03:22:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0143e480210.1371/journal.pone.0004802Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.Han SiRamandeep S BangaPinelopi KapitsinouManjunath RamaiahJanis LawrenceGanesh KambhampatiAntje GruenwaldErwin BottingerDaniel GlicklichVivian TellisStuart GreensteinDavid B ThomasJames PullmanMelissa FazzariKatalin SusztakThe incidence of End Stage Renal Disease (ESRD) is approximately 50% higher in men than women. In order to understand the molecular basis of this gender disparity, we examined sex specific gene expression patterns in control and diseased, human and murine kidney samples. Using the Affymetrix platform we performed comprehensive gene expression analysis on 42 microdissected human kidney samples (glomeruli and tubules). We identified 67 genes with gender biased expression in healthy human kidneys and 24 transcripts in diseased male and female human kidneys. Similar analysis performed in mice using male and female control and doxorubicin induced nephrotic syndrome kidneys identified significantly larger number of differentially expressed transcripts. The majority of genes showing gender biased expression either in diseased human and murine kidneys were different from those differentially expressed in healthy kidneys. Only 9 sexually dimorphic transcripts were common to healthy human and murine kidneys and five showed differential regulation in both human and murine diseased kidneys. In humans, sex biased genes showed statistical enrichment only to sex chromosomes while in mice they were enriched to sex chromosomes and various autosomes. Thus we present a comprehensive analysis of gender biased genes in the kidney. We show that sexually dimorphic genes in the kidney show species specific regulation. Our results also indicate that male and female kidneys respond differently to injury. These studies could provide the basis for the development of new treatment strategies for men and women with kidney disease.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004802&type=printable
spellingShingle Han Si
Ramandeep S Banga
Pinelopi Kapitsinou
Manjunath Ramaiah
Janis Lawrence
Ganesh Kambhampati
Antje Gruenwald
Erwin Bottinger
Daniel Glicklich
Vivian Tellis
Stuart Greenstein
David B Thomas
James Pullman
Melissa Fazzari
Katalin Susztak
Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
PLoS ONE
title Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
title_full Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
title_fullStr Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
title_full_unstemmed Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
title_short Human and murine kidneys show gender- and species-specific gene expression differences in response to injury.
title_sort human and murine kidneys show gender and species specific gene expression differences in response to injury
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004802&type=printable
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