Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection
Background: Circulating degranulated platelets have been described during acute SARS-CoV-2 infection and associated with COVID-19 complications. This study investigated the relationship between the presence of plasma SARS-CoV-2 RNA (ie, SARS-CoV-2 RNAemia), systemic inflammation, and platelet dysf...
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| Format: | Article |
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Case Western Reserve University
2025-07-01
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| Series: | Pathogens and Immunity |
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| Online Access: | https://www.paijournal.com/index.php/paijournal/article/view/823 |
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| author | Mariangela Scavone Roberta Rovito Claudia Ghali Antonella Fioretti Bianca Clerici Elena Bossi Camilla Tincati Andrea Santoro Elisa Borghi Gianmarco Podda Giulia Marchetti |
| author_facet | Mariangela Scavone Roberta Rovito Claudia Ghali Antonella Fioretti Bianca Clerici Elena Bossi Camilla Tincati Andrea Santoro Elisa Borghi Gianmarco Podda Giulia Marchetti |
| author_sort | Mariangela Scavone |
| collection | DOAJ |
| description |
Background: Circulating degranulated platelets have been described during acute SARS-CoV-2 infection and associated with COVID-19 complications. This study investigated the relationship between the presence of plasma SARS-CoV-2 RNA (ie, SARS-CoV-2 RNAemia), systemic
inflammation, and platelet dysfunction in a group of patients with COVID-19. Unlike our previous publication, which focused on platelet characterization, this work explores potential determinants of platelet activation, based on a distinct subset of patients with available stored samples.
Methods: Patients with COVID-19 were stratified by platelet δ-granule content using the luciferin/luciferase assay into 2 groups: normal (COVδ-norm) and low (COVδ-low). Plasma SARS-CoV-2 RNAemia (RT-qPCR), cytokines, and chemokines (Cytometric Bead Array) were quantified on plasma samples. Markers of platelet activation were measured by flow cytometry in whole blood.
Results: A total of 75 patients with COVID-19 were enrolled; 57 presented normal levels of platelet δ-granule content (COVδ-norm) and 18 had low levels of platelet δ-granules (COVδ-low). Groups were comparable in terms of age, sex, comorbidities, and SARS-CoV-2 RNAemia levels. Patients in the COVδ-low group showed significantly higher chemokine and cytokine levels compared to those in the COVδ-norm group, with strong correlations between IL-6, as well as granulocyte-macrophage colony-stimulating factor (GM-CSF), with platelet degranulation parameters. A similar trend, albeit less pronounced, was observed when patients were stratified based on their platelet activation phenotype.
Conclusions: These findings suggest that peripheral inflammation, rather than SARS-CoV-2 RNAemia, is associated with platelet dysfunction during acute SARS-CoV-2 infection.
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| format | Article |
| id | doaj-art-d1938482fcc841aba9315290dfdff23c |
| institution | Kabale University |
| issn | 2469-2964 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Case Western Reserve University |
| record_format | Article |
| series | Pathogens and Immunity |
| spelling | doaj-art-d1938482fcc841aba9315290dfdff23c2025-08-20T03:33:35ZengCase Western Reserve UniversityPathogens and Immunity2469-29642025-07-0110210.20411/pai.v10i2.823Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 InfectionMariangela Scavone0https://orcid.org/0000-0002-8421-0210Roberta Rovito1Claudia Ghali2https://orcid.org/0000-0003-3124-3403Antonella Fioretti3Bianca Clerici4https://orcid.org/0000-0001-7733-4584Elena Bossi5Camilla Tincati6Andrea Santoro7Elisa Borghi8Gianmarco Podda9Giulia Marchetti10Division of General Medicine II, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Milan, ItalyClinic of Infectious Diseases and Tropical Medicine, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, ItalyDivision of General Medicine II, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Milan, ItalyDivision of General Medicine II, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Milan, ItalyClinic of Infectious Diseases and Tropical Medicine, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Italy Division of General Medicine II, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Milan, ItalyClinic of Infectious Diseases and Tropical Medicine, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Italy Clinic of Infectious Diseases and Tropical Medicine, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Italy Clinical Microbiology, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, ItalyDivision of General Medicine II, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Milan, Italy"Clinic of Infectious Diseases and Tropical Medicine, Department of Health Sciences, ASST Santi Paolo e Carlo, University of Milan, Italy Background: Circulating degranulated platelets have been described during acute SARS-CoV-2 infection and associated with COVID-19 complications. This study investigated the relationship between the presence of plasma SARS-CoV-2 RNA (ie, SARS-CoV-2 RNAemia), systemic inflammation, and platelet dysfunction in a group of patients with COVID-19. Unlike our previous publication, which focused on platelet characterization, this work explores potential determinants of platelet activation, based on a distinct subset of patients with available stored samples. Methods: Patients with COVID-19 were stratified by platelet δ-granule content using the luciferin/luciferase assay into 2 groups: normal (COVδ-norm) and low (COVδ-low). Plasma SARS-CoV-2 RNAemia (RT-qPCR), cytokines, and chemokines (Cytometric Bead Array) were quantified on plasma samples. Markers of platelet activation were measured by flow cytometry in whole blood. Results: A total of 75 patients with COVID-19 were enrolled; 57 presented normal levels of platelet δ-granule content (COVδ-norm) and 18 had low levels of platelet δ-granules (COVδ-low). Groups were comparable in terms of age, sex, comorbidities, and SARS-CoV-2 RNAemia levels. Patients in the COVδ-low group showed significantly higher chemokine and cytokine levels compared to those in the COVδ-norm group, with strong correlations between IL-6, as well as granulocyte-macrophage colony-stimulating factor (GM-CSF), with platelet degranulation parameters. A similar trend, albeit less pronounced, was observed when patients were stratified based on their platelet activation phenotype. Conclusions: These findings suggest that peripheral inflammation, rather than SARS-CoV-2 RNAemia, is associated with platelet dysfunction during acute SARS-CoV-2 infection. https://www.paijournal.com/index.php/paijournal/article/view/823COVID-19Platelets DysfunctionSARS-CoV-2 viral RNAChemokines and CytokinesInflammation |
| spellingShingle | Mariangela Scavone Roberta Rovito Claudia Ghali Antonella Fioretti Bianca Clerici Elena Bossi Camilla Tincati Andrea Santoro Elisa Borghi Gianmarco Podda Giulia Marchetti Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection Pathogens and Immunity COVID-19 Platelets Dysfunction SARS-CoV-2 viral RNA Chemokines and Cytokines Inflammation |
| title | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
| title_full | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
| title_fullStr | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
| title_full_unstemmed | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
| title_short | Investigating the Interplay of SARS-CoV-2 RNAemia and Peripheral Inflammation in Platelet Dysfunction During Acute SARS-CoV-2 Infection |
| title_sort | investigating the interplay of sars cov 2 rnaemia and peripheral inflammation in platelet dysfunction during acute sars cov 2 infection |
| topic | COVID-19 Platelets Dysfunction SARS-CoV-2 viral RNA Chemokines and Cytokines Inflammation |
| url | https://www.paijournal.com/index.php/paijournal/article/view/823 |
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