Production of maleylpyruvate and fumarylpyruvate
Gentisate 1,2-dioxygenase (GDO, EC1.14.13) from Klebsiella pneumoniae M5a1 successfully converted gentisate to maleylpyruvate. This biotransformation was performed by using genetically engineered E. coli strain carrying a gene encoding gentisate 1,2-dioxygenase, and the maleylpyruvate synthesized by...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2005-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.03.0301 |
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| Summary: | Gentisate 1,2-dioxygenase (GDO, EC1.14.13) from Klebsiella pneumoniae M5a1 successfully converted gentisate to maleylpyruvate. This biotransformation was performed by using genetically engineered E. coli strain carrying a gene encoding gentisate 1,2-dioxygenase, and the maleylpyruvate synthesized by this enzyme attained a purity as high as 97.7%. Purity of fumarylpyruvate obtained by acidifying the resulting maleylpyruvate with meta-phosphoric acid was 96.4%. This study thus established a method which is suitable for the production of maleylpyruvate and fumarylpyruvate. |
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| ISSN: | 1008-9209 2097-5155 |