Regulatory Effects of Source–Sink Manipulations on Photosynthesis in Wheat with Different Source–Sink Relationships

Understanding the contributions of source–sink relationships to photosynthesis will help achieve high wheat grain yields. A single-factor field experiment was conducted to quantify the regulatory effects of different sink–source ratios on wheat photosynthetic characteristics, including two wheat cul...

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Main Authors: Siqi Zhang, Huimin Chai, Jiawei Sun, Yafang Zhang, Yanhua Lu, Dong Jiang, Tingbo Dai, Zhongwei Tian
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/10/1456
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Summary:Understanding the contributions of source–sink relationships to photosynthesis will help achieve high wheat grain yields. A single-factor field experiment was conducted to quantify the regulatory effects of different sink–source ratios on wheat photosynthetic characteristics, including two wheat cultivars with different source–sink relationships as materials for detailed source–sink manipulations through flag leaf removal (LR) and removal of spikelets on one side of each spike (SR). Compared with a control (CK), LR increased the sink–source ratio (23.84%) and significantly reduced the yield (16.17%), 1000-kernel weight (11.73%), and kernels per spike (7.33%). LR increased the leaves’ net photosynthetic rate (Pn) (4.27–15.82%), the electron transfer rate (3.97–14.93%), and the Rubisco activity (2.16–12.25%) in the short term, and LR increased sucrose synthesis-related enzyme activities (3.96–19.95%) and gene expressions <i>(SPS1</i>, <i>SUS1</i>, <i>CIN1</i>, and <i>SUT1</i>). Compared with CK, SR reduced the sink–source ratio (44.12%) and significantly increased the 1000-kernel weight (10.02%) but reduced the yield (43.93%) and kernels per spike (49.31%). SR reduced the leaves’ Pn (8.54–21.41%), the electron transfer rate (3.51–16.71%), and the Rubisco activity (5.96–21.51%), and the photosynthetic process was limited. SR decreased sucrose synthesis-related enzyme activities (5.12–29.09%) and gene expressions <i>(SPS1</i>, <i>SUS1</i>, <i>CIN1</i>, and <i>SUT1</i>). Therefore, a higher sink–source ratio is an important indicator of high photosynthetic efficiency, which can be used as a screening and judgment index in variety selection and cultivation regulation.
ISSN:2223-7747