Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis)
The effects of various combinations of heat shock temperature and duration, medium renovation and cold shock pretreatment on the embryogenesis of isolated microspores were studied in two F<sub>1</sub> hybrids of winter cauliflower (Brassica oleracea var. botrytis). The results indicated...
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Zhejiang University Press
2004-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0034 |
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| author | GU Hong-hui TANG Gui-xiang ZHANG Guo-qing ZHOU Wei-jun |
| author_facet | GU Hong-hui TANG Gui-xiang ZHANG Guo-qing ZHOU Wei-jun |
| author_sort | GU Hong-hui |
| collection | DOAJ |
| description | The effects of various combinations of heat shock temperature and duration, medium renovation and cold shock pretreatment on the embryogenesis of isolated microspores were studied in two F<sub>1</sub> hybrids of winter cauliflower (Brassica oleracea var. botrytis). The results indicated that the optimal combination of heat shock temperature and duration was 32℃ and 24 h. A decrease in sucrose concentration from 17% to 10% in liquid NLN medium following the first 24 h incubation at 32℃ significantly enhanced microspore embryogenesis. Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos. After 24 h induction at 32℃ and continuous culture at 24℃ in darkness for 30 d, isolated microspores produced normal embryos, which were then transferred to solid MS medium for embryo germination and root development. After two times of subculture, large amounts of normal regenerated plants were produced from generated shoots, and these regenerated plants were mainly spontaneous doubled haploids. |
| format | Article |
| id | doaj-art-d16143dcd7ae41c198f4309b4b0c8647 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-d16143dcd7ae41c198f4309b4b0c86472025-08-20T02:52:51ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-01-0130343810.3785/1008-9209.2004.01.003410089209Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis)GU Hong-huiTANG Gui-xiangZHANG Guo-qingZHOU Wei-junThe effects of various combinations of heat shock temperature and duration, medium renovation and cold shock pretreatment on the embryogenesis of isolated microspores were studied in two F<sub>1</sub> hybrids of winter cauliflower (Brassica oleracea var. botrytis). The results indicated that the optimal combination of heat shock temperature and duration was 32℃ and 24 h. A decrease in sucrose concentration from 17% to 10% in liquid NLN medium following the first 24 h incubation at 32℃ significantly enhanced microspore embryogenesis. Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos. After 24 h induction at 32℃ and continuous culture at 24℃ in darkness for 30 d, isolated microspores produced normal embryos, which were then transferred to solid MS medium for embryo germination and root development. After two times of subculture, large amounts of normal regenerated plants were produced from generated shoots, and these regenerated plants were mainly spontaneous doubled haploids.https://www.academax.com/doi/10.3785/1008-9209.2004.01.0034<italic>Brassica oleracea</italic> var. <italic>botrytis</italic>microspore cultureembryogenesisplant regenerationploidy testing |
| spellingShingle | GU Hong-hui TANG Gui-xiang ZHANG Guo-qing ZHOU Wei-jun Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) 浙江大学学报. 农业与生命科学版 <italic>Brassica oleracea</italic> var. <italic>botrytis</italic> microspore culture embryogenesis plant regeneration ploidy testing |
| title | Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) |
| title_full | Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) |
| title_fullStr | Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) |
| title_full_unstemmed | Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) |
| title_short | Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis) |
| title_sort | embryogenesis and plant regeneration from isolated microspores of winter cauliflower brassica oleracea var botrytis |
| topic | <italic>Brassica oleracea</italic> var. <italic>botrytis</italic> microspore culture embryogenesis plant regeneration ploidy testing |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.01.0034 |
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