CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis
Abstract A hallmark of glioma cells, particularly glioblastoma multiforme (GBM) cells, is their resistance to apoptosis. Accumulating evidences has demonstrated that CARD16, a caspase recruitment domain (CARD) only protein, enhances both anti-apoptotic and tumorigenic properties. Nevertheless, there...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2024-11-01
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| Series: | Cell Death and Disease |
| Online Access: | https://doi.org/10.1038/s41419-024-07196-2 |
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| _version_ | 1850061912138579968 |
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| author | Ruoheng Xuan Tianyu Hu Lingshan Cai Beichuan Zhao Erqiao Han Zhibo Xia |
| author_facet | Ruoheng Xuan Tianyu Hu Lingshan Cai Beichuan Zhao Erqiao Han Zhibo Xia |
| author_sort | Ruoheng Xuan |
| collection | DOAJ |
| description | Abstract A hallmark of glioma cells, particularly glioblastoma multiforme (GBM) cells, is their resistance to apoptosis. Accumulating evidences has demonstrated that CARD16, a caspase recruitment domain (CARD) only protein, enhances both anti-apoptotic and tumorigenic properties. Nevertheless, there is a limited understanding of the expression and functional role of CARD16 in glioma. This study seeks to investigate, through in silico analysis and clinical specimens, the role of CARD16 as a potential tumor promoter in glioma. Functional assays and molecular studies revealed that CARD16 promotes tumorigenesis and suppresses apoptosis in glioma cells. Moreover, knockdown of CARD16 enhances the expression of the FOXO1/TRAIL axis in GBM cells. Additionally, FOXO1 downregulation in CARD16 knockdown GBM cells restores proliferation and reduces apoptosis. Further investigation demonstrated that elevated P21 expression inhibits CDK2-mediated FOXO1 phosphorylation and ubiquitination in CARD16-knockdown GBM cells. Collectively, these findings suggest that CARD16 is a tumor-promoting molecular in glioma via downregulating FOXO1/TRAIL axis, and suppressing TRAIL-induced apoptosis. The CARD16 gene presents significant potential for prognostic prediction and advances in innovative apoptotic therapeutics. |
| format | Article |
| id | doaj-art-d15be9c8079c41c4b3cd79ff0733af80 |
| institution | DOAJ |
| issn | 2041-4889 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death and Disease |
| spelling | doaj-art-d15be9c8079c41c4b3cd79ff0733af802025-08-20T02:50:04ZengNature Publishing GroupCell Death and Disease2041-48892024-11-01151111410.1038/s41419-024-07196-2CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axisRuoheng Xuan0Tianyu Hu1Lingshan Cai2Beichuan Zhao3Erqiao Han4Zhibo Xia5Department of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen UniversityAbstract A hallmark of glioma cells, particularly glioblastoma multiforme (GBM) cells, is their resistance to apoptosis. Accumulating evidences has demonstrated that CARD16, a caspase recruitment domain (CARD) only protein, enhances both anti-apoptotic and tumorigenic properties. Nevertheless, there is a limited understanding of the expression and functional role of CARD16 in glioma. This study seeks to investigate, through in silico analysis and clinical specimens, the role of CARD16 as a potential tumor promoter in glioma. Functional assays and molecular studies revealed that CARD16 promotes tumorigenesis and suppresses apoptosis in glioma cells. Moreover, knockdown of CARD16 enhances the expression of the FOXO1/TRAIL axis in GBM cells. Additionally, FOXO1 downregulation in CARD16 knockdown GBM cells restores proliferation and reduces apoptosis. Further investigation demonstrated that elevated P21 expression inhibits CDK2-mediated FOXO1 phosphorylation and ubiquitination in CARD16-knockdown GBM cells. Collectively, these findings suggest that CARD16 is a tumor-promoting molecular in glioma via downregulating FOXO1/TRAIL axis, and suppressing TRAIL-induced apoptosis. The CARD16 gene presents significant potential for prognostic prediction and advances in innovative apoptotic therapeutics.https://doi.org/10.1038/s41419-024-07196-2 |
| spellingShingle | Ruoheng Xuan Tianyu Hu Lingshan Cai Beichuan Zhao Erqiao Han Zhibo Xia CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis Cell Death and Disease |
| title | CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis |
| title_full | CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis |
| title_fullStr | CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis |
| title_full_unstemmed | CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis |
| title_short | CARD16 restores tumorigenesis and restraints apoptosis in glioma cells Via FOXO1/TRAIL axis |
| title_sort | card16 restores tumorigenesis and restraints apoptosis in glioma cells via foxo1 trail axis |
| url | https://doi.org/10.1038/s41419-024-07196-2 |
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