Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators

Alfalfa (<i>Medicago sativa</i>) is an important fodder crop with 16–25% protein and 20–30% fibre content. It requires pollinators for seed development through a process known as “tripping”. Honey bees are preferred for their easy management and mass rearing to enhance pollination. In a...

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Main Authors: Kamran Ejaz, Mudssar Ali, Fawad Zafar Ahmad Khan, Raimondas Mozūratis
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/14/6/599
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author Kamran Ejaz
Mudssar Ali
Fawad Zafar Ahmad Khan
Raimondas Mozūratis
author_facet Kamran Ejaz
Mudssar Ali
Fawad Zafar Ahmad Khan
Raimondas Mozūratis
author_sort Kamran Ejaz
collection DOAJ
description Alfalfa (<i>Medicago sativa</i>) is an important fodder crop with 16–25% protein and 20–30% fibre content. It requires pollinators for seed development through a process known as “tripping”. Honey bees are preferred for their easy management and mass rearing to enhance pollination. In a field experiment, we tested three treatments: two honey bee colonies (moderately supplemented), three honey bee colonies (highly supplemented), and a control with no honey bee colonies. The colonies European honey bee, <i>A. mellifera</i>, were used, starting at the flowering stage. We assessed the abundance, foraging, and tripping behaviour of pollinators, as well as the number of seeds per pod, pod weight, and seed weight. Honey bees were the most abundant in supplemented plots, followed by solitary bees, with increased honey bee visitation in the second year. <i>Megachile cephalotes</i>, <i>M. hera</i>, and <i>A. mellifera</i> were the most efficient pollinators regarding seeds per pod and seed weight. The plot with two honey bee colonies had a higher seed yield than the one with three colonies. Conservation of these pollinators and the introduction of managed <i>A. mellifera</i> colonies can lead to sustainable alfalfa seed production. Our findings suggest that honey bee supplementation, along with conservation efforts for solitary bees, can enhance pollination and seed yield in alfalfa.
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spelling doaj-art-d151ef5cbddc4847814c9eea964343662025-08-20T03:27:09ZengMDPI AGBiology2079-77372025-05-0114659910.3390/biology14060599Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other PollinatorsKamran Ejaz0Mudssar Ali1Fawad Zafar Ahmad Khan2Raimondas Mozūratis3Institute of Plant Protection, Muhammad Nawaz Shareef University of Agriculture Multan, Multan 60000, PakistanInstitute of Plant Protection, Muhammad Nawaz Shareef University of Agriculture Multan, Multan 60000, PakistanInstitute of Plant Protection, Muhammad Nawaz Shareef University of Agriculture Multan, Multan 60000, PakistanDepartment of Zoology, Stockholm University, SE-10691 Stockholm, SwedenAlfalfa (<i>Medicago sativa</i>) is an important fodder crop with 16–25% protein and 20–30% fibre content. It requires pollinators for seed development through a process known as “tripping”. Honey bees are preferred for their easy management and mass rearing to enhance pollination. In a field experiment, we tested three treatments: two honey bee colonies (moderately supplemented), three honey bee colonies (highly supplemented), and a control with no honey bee colonies. The colonies European honey bee, <i>A. mellifera</i>, were used, starting at the flowering stage. We assessed the abundance, foraging, and tripping behaviour of pollinators, as well as the number of seeds per pod, pod weight, and seed weight. Honey bees were the most abundant in supplemented plots, followed by solitary bees, with increased honey bee visitation in the second year. <i>Megachile cephalotes</i>, <i>M. hera</i>, and <i>A. mellifera</i> were the most efficient pollinators regarding seeds per pod and seed weight. The plot with two honey bee colonies had a higher seed yield than the one with three colonies. Conservation of these pollinators and the introduction of managed <i>A. mellifera</i> colonies can lead to sustainable alfalfa seed production. Our findings suggest that honey bee supplementation, along with conservation efforts for solitary bees, can enhance pollination and seed yield in alfalfa.https://www.mdpi.com/2079-7737/14/6/599beesfodderforaging behaviourtripping behaviour
spellingShingle Kamran Ejaz
Mudssar Ali
Fawad Zafar Ahmad Khan
Raimondas Mozūratis
Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
Biology
bees
fodder
foraging behaviour
tripping behaviour
title Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
title_full Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
title_fullStr Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
title_full_unstemmed Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
title_short Enhancing Alfalfa (<i>Medicago sativa</i>) Seed Yield: The Effect of Honey Bee (<i>Apis mellifera</i>) Supplementation and Efficiency of Other Pollinators
title_sort enhancing alfalfa i medicago sativa i seed yield the effect of honey bee i apis mellifera i supplementation and efficiency of other pollinators
topic bees
fodder
foraging behaviour
tripping behaviour
url https://www.mdpi.com/2079-7737/14/6/599
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