Does salivary microbiome reflect the functional activity profile of oral microbiota?
Human oral cavity is a diverse habitat, consisting of many locations with its microbiotas. It was proven that bacteria detected in saliva could be the indicators of disease and be useful for diagnosis, monitoring, and overall assessment of the patient health. As pilot research of microbial communiti...
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| Format: | Article |
| Language: | English |
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Limited liability company «Science and Innovations» (Saratov)
2022-03-01
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| Series: | Russian Open Medical Journal |
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| Online Access: | https://romj.org/node/433 |
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| author | Elizaveta S. Klimenko Ilya A. Igumnov Daria P. Markova Natalia L. Belkova Larisa V. Suturina |
| author_facet | Elizaveta S. Klimenko Ilya A. Igumnov Daria P. Markova Natalia L. Belkova Larisa V. Suturina |
| author_sort | Elizaveta S. Klimenko |
| collection | DOAJ |
| description | Human oral cavity is a diverse habitat, consisting of many locations with its microbiotas. It was proven that bacteria detected in saliva could be the indicators of disease and be useful for diagnosis, monitoring, and overall assessment of the patient health. As pilot research of microbial communities associated with polycystic ovary syndrome (PCOS), biomaterials were collected from two patients (saliva, the contents of gingival pockets) for sequencing the amplicon libraries V4-V6 of variable regions of the 16S rRNA gene. Our study aimed at comparing the taxonomic and functional profiles of different locations in the oral cavity.
Material and Methods — DNA samples were sequenced using Illumina MiSeq; the qiime2-2020.2 and PICRUSt2 v2.4.1 software packages were used to process the sequencing results of the amplicon libraries.
Results — We demonstrated that the salivary microbiome had a greater taxonomic diversity, compared with the microbiome of the periodontal pockets. Regarding the ratios of different taxonomic group abundances, the species ratio in the saliva community significantly differed from the ratio in periodontal pockets. The microbiota of the oral cavity was classified as a producing community, since many different biosynthetic pathways were predicted. Similar functional features were identified for microbial communities in other locations.
Conclusions — Different locations in the biotope of the oral cavity have varying species richness of their communities and specific taxonomic composition. However, the microbiotas of different microniches perform similar metabolic functions. This finding allows considering the analysis of saliva microbiota sufficiently representative tool for characterization of the entire oral microbiome. |
| format | Article |
| id | doaj-art-d145a32b025d44afb5af296fea082ddf |
| institution | DOAJ |
| issn | 2304-3415 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Limited liability company «Science and Innovations» (Saratov) |
| record_format | Article |
| series | Russian Open Medical Journal |
| spelling | doaj-art-d145a32b025d44afb5af296fea082ddf2025-08-20T03:19:17ZengLimited liability company «Science and Innovations» (Saratov)Russian Open Medical Journal2304-34152022-03-01111e012010.15275/rusomj.2022.0120Does salivary microbiome reflect the functional activity profile of oral microbiota?Elizaveta S. KlimenkoIlya A. IgumnovDaria P. MarkovaNatalia L. BelkovaLarisa V. SuturinaHuman oral cavity is a diverse habitat, consisting of many locations with its microbiotas. It was proven that bacteria detected in saliva could be the indicators of disease and be useful for diagnosis, monitoring, and overall assessment of the patient health. As pilot research of microbial communities associated with polycystic ovary syndrome (PCOS), biomaterials were collected from two patients (saliva, the contents of gingival pockets) for sequencing the amplicon libraries V4-V6 of variable regions of the 16S rRNA gene. Our study aimed at comparing the taxonomic and functional profiles of different locations in the oral cavity. Material and Methods — DNA samples were sequenced using Illumina MiSeq; the qiime2-2020.2 and PICRUSt2 v2.4.1 software packages were used to process the sequencing results of the amplicon libraries. Results — We demonstrated that the salivary microbiome had a greater taxonomic diversity, compared with the microbiome of the periodontal pockets. Regarding the ratios of different taxonomic group abundances, the species ratio in the saliva community significantly differed from the ratio in periodontal pockets. The microbiota of the oral cavity was classified as a producing community, since many different biosynthetic pathways were predicted. Similar functional features were identified for microbial communities in other locations. Conclusions — Different locations in the biotope of the oral cavity have varying species richness of their communities and specific taxonomic composition. However, the microbiotas of different microniches perform similar metabolic functions. This finding allows considering the analysis of saliva microbiota sufficiently representative tool for characterization of the entire oral microbiome.https://romj.org/node/433oral microbiomepcos16s rrna geneprotein function prediction |
| spellingShingle | Elizaveta S. Klimenko Ilya A. Igumnov Daria P. Markova Natalia L. Belkova Larisa V. Suturina Does salivary microbiome reflect the functional activity profile of oral microbiota? Russian Open Medical Journal oral microbiome pcos 16s rrna gene protein function prediction |
| title | Does salivary microbiome reflect the functional activity profile of oral microbiota? |
| title_full | Does salivary microbiome reflect the functional activity profile of oral microbiota? |
| title_fullStr | Does salivary microbiome reflect the functional activity profile of oral microbiota? |
| title_full_unstemmed | Does salivary microbiome reflect the functional activity profile of oral microbiota? |
| title_short | Does salivary microbiome reflect the functional activity profile of oral microbiota? |
| title_sort | does salivary microbiome reflect the functional activity profile of oral microbiota |
| topic | oral microbiome pcos 16s rrna gene protein function prediction |
| url | https://romj.org/node/433 |
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