Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients
This study was conducted to purifity Glucose 6-phosphate dehydrogenase from diabetic patients by gel filtration technique on Sephadex G100 and determine the molecular weight of enzyme, concentration and kinetic constant [Km, Vmax], study the effect of optimum temperature, substrate, pH. The study...
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Tikrit University
2018-05-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/501 |
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| author | Abdulkader W. Rasheed Firas T. Maher Firas T. Maher Akeel H. Al-Aisse |
| author_facet | Abdulkader W. Rasheed Firas T. Maher Firas T. Maher Akeel H. Al-Aisse |
| author_sort | Abdulkader W. Rasheed |
| collection | DOAJ |
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This study was conducted to purifity Glucose 6-phosphate dehydrogenase from diabetic patients by gel filtration technique on Sephadex G100 and determine the molecular weight of enzyme, concentration and kinetic constant [Km, Vmax], study the effect of optimum temperature, substrate, pH. The study includes [60] patients with diabetes mellitus and [60] healthy individual as control. The activity of G6PD was measured after precipitated by [75%] Ammonium Sulfate concentration, using [Tris-HCl] buffer at pH 8.2. The specific activity was calculated [21.5UI\mg], total activity [706.8UI], number of purification [3.45] enzyme yield [23.188%] and enzyme activity [17.67UI\ml]. and the molecular weight were [57.82] k.D. The effect of substrate concentration was found to be increased with increasing substrate concentration. The optimum pH was found to be [8.4] and the optimum temperature was [38C]. Michaelis- Menten [Km] value for the enzyme was [3.33mM] and Vmax value was [0.263IU\ml ].
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| format | Article |
| id | doaj-art-d143ca1b3fdb412eb7947243428b96cc |
| institution | DOAJ |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-d143ca1b3fdb412eb7947243428b96cc2025-08-20T03:11:42ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262018-05-0123310.25130/tjps.v23i3.501Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patientsAbdulkader W. RasheedFiras T. MaherFiras T. MaherAkeel H. Al-Aisse This study was conducted to purifity Glucose 6-phosphate dehydrogenase from diabetic patients by gel filtration technique on Sephadex G100 and determine the molecular weight of enzyme, concentration and kinetic constant [Km, Vmax], study the effect of optimum temperature, substrate, pH. The study includes [60] patients with diabetes mellitus and [60] healthy individual as control. The activity of G6PD was measured after precipitated by [75%] Ammonium Sulfate concentration, using [Tris-HCl] buffer at pH 8.2. The specific activity was calculated [21.5UI\mg], total activity [706.8UI], number of purification [3.45] enzyme yield [23.188%] and enzyme activity [17.67UI\ml]. and the molecular weight were [57.82] k.D. The effect of substrate concentration was found to be increased with increasing substrate concentration. The optimum pH was found to be [8.4] and the optimum temperature was [38C]. Michaelis- Menten [Km] value for the enzyme was [3.33mM] and Vmax value was [0.263IU\ml ]. https://tjpsj.org/index.php/tjps/article/view/501G6PDDiabetes mellitusGel filtration chromatographymolecular weight |
| spellingShingle | Abdulkader W. Rasheed Firas T. Maher Firas T. Maher Akeel H. Al-Aisse Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients Tikrit Journal of Pure Science G6PD Diabetes mellitus Gel filtration chromatography molecular weight |
| title | Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients |
| title_full | Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients |
| title_fullStr | Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients |
| title_full_unstemmed | Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients |
| title_short | Purification and determination the molecular weight and study the kinetic properties of Glucose-6-phosphate dehydrogenase from Diabetic patients |
| title_sort | purification and determination the molecular weight and study the kinetic properties of glucose 6 phosphate dehydrogenase from diabetic patients |
| topic | G6PD Diabetes mellitus Gel filtration chromatography molecular weight |
| url | https://tjpsj.org/index.php/tjps/article/view/501 |
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