Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum

To investigate the compositional characteristics of microbial communities in the rhizosphere soil of Cymbidium tracyanum (C. tracyanum) across different production regions in Tibet, as well as the correlation between these microorganisms and soil physicochemical factors, we analyzed soil microbial c...

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Main Authors: Wenwen Xie, Ying Tang, Haiming Li, Mingyu Dang, Jianuo Ci, Min Zheng, Erhao Zhang, Zhongbin Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1519614/full
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author Wenwen Xie
Ying Tang
Haiming Li
Mingyu Dang
Jianuo Ci
Min Zheng
Erhao Zhang
Zhongbin Wang
author_facet Wenwen Xie
Ying Tang
Haiming Li
Mingyu Dang
Jianuo Ci
Min Zheng
Erhao Zhang
Zhongbin Wang
author_sort Wenwen Xie
collection DOAJ
description To investigate the compositional characteristics of microbial communities in the rhizosphere soil of Cymbidium tracyanum (C. tracyanum) across different production regions in Tibet, as well as the correlation between these microorganisms and soil physicochemical factors, we analyzed soil microbial community composition in Bayi District, Chayu County, and Mêdog County in Linzhi City, Tibet, using Illumina MiSeq high-throughput sequencing technology. The results indicate that 7,467 bacterial operational taxonomic units (OTUs) and 2,866 fungal OTUs were identified in the rhizosphere soil of C. tracyanum. Significant differences were observed in the structural composition of bacterial and fungal communities across the different regions. The dominant bacterial phyla in the rhizosphere soil included Proteobacteria, Acidobacteria, Planctomycetota, and Firmicutes, while Ascomycota and Basidiomycota were the predominant fungal phyla. Additionally, variations in the physicochemical properties of the rhizosphere soil were observed among the different regions. Core microbiota analysis identified 214 core bacterial genera and 79 core fungal genera in the rhizosphere soil of C. tracyanum in Tibet. Correlation analysis revealed that changes in the core microbial community were associated with soil physicochemical factors to varying degrees, with total phosphorus and available phosphorus emerging as key factors influencing microbial diversity in the rhizosphere soil. In summary, the composition and diversity of bacterial and fungal communities in the rhizosphere soil of C. tracyanum varied across different production regions, and shifts in microbial community structure were closely linked to soil physicochemical factors.
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spelling doaj-art-d12f2029fb324cfa857f80d8a9546cea2025-08-20T02:11:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15196141519614Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanumWenwen Xie0Ying Tang1Haiming Li2Mingyu Dang3Jianuo Ci4Min Zheng5Erhao Zhang6Zhongbin Wang7School of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaCollege of Food Science, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaSchool of Resources and Environment, Tibet Agriculture and Animal Husbandry College, Linzhi, ChinaTo investigate the compositional characteristics of microbial communities in the rhizosphere soil of Cymbidium tracyanum (C. tracyanum) across different production regions in Tibet, as well as the correlation between these microorganisms and soil physicochemical factors, we analyzed soil microbial community composition in Bayi District, Chayu County, and Mêdog County in Linzhi City, Tibet, using Illumina MiSeq high-throughput sequencing technology. The results indicate that 7,467 bacterial operational taxonomic units (OTUs) and 2,866 fungal OTUs were identified in the rhizosphere soil of C. tracyanum. Significant differences were observed in the structural composition of bacterial and fungal communities across the different regions. The dominant bacterial phyla in the rhizosphere soil included Proteobacteria, Acidobacteria, Planctomycetota, and Firmicutes, while Ascomycota and Basidiomycota were the predominant fungal phyla. Additionally, variations in the physicochemical properties of the rhizosphere soil were observed among the different regions. Core microbiota analysis identified 214 core bacterial genera and 79 core fungal genera in the rhizosphere soil of C. tracyanum in Tibet. Correlation analysis revealed that changes in the core microbial community were associated with soil physicochemical factors to varying degrees, with total phosphorus and available phosphorus emerging as key factors influencing microbial diversity in the rhizosphere soil. In summary, the composition and diversity of bacterial and fungal communities in the rhizosphere soil of C. tracyanum varied across different production regions, and shifts in microbial community structure were closely linked to soil physicochemical factors.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1519614/fullCymbidium tracyanum (C. Tracyanum)rhizosphere soil microorganismshigh-throughput sequencingcore microbessoil physicochemical factors
spellingShingle Wenwen Xie
Ying Tang
Haiming Li
Mingyu Dang
Jianuo Ci
Min Zheng
Erhao Zhang
Zhongbin Wang
Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
Frontiers in Microbiology
Cymbidium tracyanum (C. Tracyanum)
rhizosphere soil microorganisms
high-throughput sequencing
core microbes
soil physicochemical factors
title Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
title_full Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
title_fullStr Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
title_full_unstemmed Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
title_short Physicochemical properties and microbial community structure of the rhizosphere soil of Cymbidium tracyanum
title_sort physicochemical properties and microbial community structure of the rhizosphere soil of cymbidium tracyanum
topic Cymbidium tracyanum (C. Tracyanum)
rhizosphere soil microorganisms
high-throughput sequencing
core microbes
soil physicochemical factors
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1519614/full
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