Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence
Summary: Dorsal root ganglion (DRG) neurons innervate the periphery of the body to transmit somatosensory information, including touch, pain, and temperature. Here, we present a protocol for isolating rat DRG neurons using an immunopanning technique. We describe steps for dissecting DRGs from embryo...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002424 |
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| Summary: | Summary: Dorsal root ganglion (DRG) neurons innervate the periphery of the body to transmit somatosensory information, including touch, pain, and temperature. Here, we present a protocol for isolating rat DRG neurons using an immunopanning technique. We describe steps for dissecting DRGs from embryonic day (E)15 rat embryos, triturating ganglionic cells, and purifying DRG neurons. We also detail procedures for mRNA or protein quantification using RNAscope and immunofluorescence. This protocol has potential applications for studying DRG neurons during development and disease.For complete details on the use and execution of this protocol, please refer to Kantarci et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |